These images show live embryos of animals (jellyfish, crustacean, worm, sea urchin, sea squirt, beetle) and one of animals closest single-celled relatives. They were captured taking advantage of fluorescent proteins localised on the outer membrane of cells, allowing us to observe cell outlines. 1/9
Now published. Congrats to Soham and all co-authors! www.science.org/doi/10.1126/....
@embl.org
Pictures from Fanny Martinez Real and Stefan Mundlos🙏
This year, the annual symposium of my Chaire @college-de-france.fr will be on The Evolution of Developmental Mechanisms. An impressive lineup of speakers and weirds animals, including humans. Free entrance and coffee breaks. Come, have a seat, relax and enjoy the best possible basic science🤘RT🙏
Our latest work on shape-programmable tissues is out in @science.org. By positioning topological defects in cellular nematics, we encode frustrated 2D force fields that relax into predictable 3D shapes. Collaboration with Marino Arroyo’s lab, led by @pauguillamat.bsky.social at @ibecbarcelona.eu.
New paper out! Looking at RNA-related pathways in neuronal remodeling, we found UAP56, an mRNA export factor. Actin disassembly is a likely target, both in dendrites and at presynapses. Also: cofilin is required for presynapse pruning. Please check out - journals.biologists.com/jcs/article/...
Drosophila border cells are beautiful to watch… but far from easy to image. Kudos to the brave and skilled @vitoryang.bsky.social, who took on the challenge of establishing border cell migration imaging in the lab! His movies are astonishing, and now our first-ever study on #cell #migration is out!
Latest preprint from @phnglab.bsky.social @riken-bdr.bsky.social ! We show cellular hydraulics as a determinant of hemogenic endothelial cell (HEC) survival during endothelial-to-hematopoietic (EHT) transition.
www.biorxiv.org/cgi/content/...
#mechanobiology #zebrafish #endothelialcells
プレプリントをbioRxivに公開しました．
細胞レベルの利き手から多細胞レベルの利き手が決まる物理メカニズムの一端を実験と理論の組み合わせから明らかにしました．
Force transmission balance through adhesions determines multicellular handedness
www.biorxiv.org/content/10.6...
みんな...読んでﾈ...
Force transmission balance through adhesions determines multicellular handedness www.biorxiv.org/content/10.64898/2026.04...
☀️ Join us next week for another great Eastern VGZT session!
🗓️ Wednesday, April 8th
⏰ 9:00 BST / 10:00 CEST / 13:30 IST / 17:00 JST / 18:00 AEST
Our speakers are:
👉 Leqian Yu
👉 Tatsuo Shibata
See you there! 👋
🐟!JOB ALERT!Duke, Durham, NC!🐟 We are looking for a research tech (our current tech is starting grad school this Fall (Yay!)). The candidate will contribute to our ongoing research projects on tissue morphogenesis using zebrafish. Please RT🙏
www.munjallab.com/join-us
The above study by Yan Chen, @phnglab.bsky.social, @labphb.bsky.social and others was published in #NatureCommunications.
"Circumferential actomyosin bundles anchored by CCM1 drive endothelial cell contraction and vessel constriction."
doi.org/10.1038/s414...
📃Read about a study led by Yan Chen & @phnglab.bsky.social revealing how circumferential actomyosin drives contraction of endothelial cells to regulate blood vessel diameter, and how vascular malformations can arise when these cellular mechanics go awry.
👇
www.bdr.riken.jp/en/news/rese...
Excited to share our new article, ‘Mechanical regulation of cuboidal-to-squamous epithelial transition in the Drosophila developing wing’, now online in Current Biology.
doi.org/10.1016/j.cu...
There’s also a great accompanying Dispatch:
doi.org/10.1016/j.cu...
#MatrixMechanics #DevBio
How does tissue mechanics drive the evolutionary diversity of forms/morphology we see around us?
Our views and review of literature on the importance of studying forms, and more
authors.elsevier.com/sd/article/S...
Our study on shape diversity in cnidarians is now published. The final version includes extensive new data that substantially extend the original bioRxiv preprint. Congrats to everyone who contributed to this work! www.cell.com/cell/fulltex...
@embl.org
To wrap, while long-time coming, very happy with its ground breaking, or at least tissue "rupturing" insights, thanks to Chun Wai's dedication, Takefumi Kondo's breakthrough scRNA-seq analysis. Much more to learn about the fascinating extraembryonic tissues of flies. Stay tuned. (9/9)
Though gastrulation evolved preventive measures to prevent rupture, increasing number of developmental contexts sees rupture as a critical mechanism. See @rashmi-priya.bsky.social's excellent review bsky.app/profile/rash... (8/9)
In sum, cell-intrinsic compliance is needed to prevent tissue rupture in contexts with strong pulling forces. Complements recent stories of ours with Steffen Lemke and @paveltomancak.bsky.social @bruvellu.bsky.social on how pushing forces are safeguarded. bsky.app/profile/yuch... (7/9)
Lo and behold when mitoTRAP turns Shroom on in amnioserosa that normally does not express it, tissue ruptures. Increased intrinsic junctional stresses rip tissue apart when under external load, echoing insightful in vitro work of @gcharras.bsky.social rdcu.be/e6q7k (6/9)
How do we establish causality? We sommon the awesome power of optogenetics – newly developed photoactivatable Gal4 system we call mitoTRAP Gal4. See how it revs up target gene expression in just 30 minutes.
How does Zen do it? By transcriptionally silencing Shroom, a powerful activator of junctional actomyosin contractility, thereby keeping intrinsic junctional stresses low and cell compliance high. (4/9)
Extreme intrinsic stretchability is ensured by the amnioserosa master regulator Zen. Powerful pulling forces wreak havoc, rupturing tissue in dramatic fashions. No Zen, no (tissue) integrity. (3/9)
Cells of extraembryonic amnioserosa are known to undergo extreme flattening and surface elongation. Turns out elongation depend on both intrinsic property and external pulling forces. Tour de force genetics by Chun Wai Kwan taking out 4 genes, no actually 5, to fully block elongation. (2/9)
Can contractile forces that internalize cells and rearrange tissues cause harm? They can, if not mitigated. We show that built-in high stretchability of the extraembryonic cells protects gastrulating Drosophila embryo from being ripped apart. (1/9) doi.org/10.64898/202...
Final version @nature.com of our paper describing unconventional multicellular development in a choanoflagellate inhabiting an extreme environment. A ton of new data since the first @biorxivpreprint.bsky.social preprint (which we've kept updating).
A brief 🧵 (carried over from the old place)
Do also check out amazing videos of cortical actin dynamics in #zebrafish #endothelialcells such as this one! @riken-bdr.bsky.social
Here's the link to the original article if you haven't checked it out already! www.nature.com/articles/s41...
Happy to share a write-up of our recent publication @natcomms.nature.com demonstrating
how circumferential actomyosin-driven endothelial cell contractions constrict vessel diameter, providing insights into mechanisms of normal vessel remodelling and CCM.
www.bdr.riken.jp/en/news/rese...
Happy to share this work from Carlos Camacho de la Maccora that reveals how rates of posterior progenitor addition and anterior vacuolation are balanced across the notochord. With Alberto Ceccarelli and @osvaldo-chara.bsky.social we present a model of long-range communication to provide robustness.
