Light sheet lab. We image anything from cells to plants to shrimp.
@fedegasparoli.bsky.social @excitedemissions.bsky.social are running the machines!

Light Sheet Saturday is here.
@jencwaters.bsky.social and @talley.codes lecture on how to improve your photon budget by illuminating from the side.

Pizza and image analysis. Because there’s no hackin‘ without snackin‘.
@damiandn.bsky.social @bethcimini.bsky.social

AI for image analysis.
@florianjug.bsky.social @bethcimini.bsky.social @damiandn.bsky.social
are showing off the power of the machines:)

Cake and image analysis. True QI bliss:)
@damiandn.bsky.social @bethcimini.bsky.social @emiglietta.bsky.social teaching 3D segmentation.

Looking for samples for our lightsheet microscope and expansion microscopy lab, @fedegasparoli.bsky.social and @lycasworks.bsky.social go on an expedition during low tide.

Confocal lab is where we show off the power of optical sectioning. Only one question remains: point scanner or spinning disc?

Hands on TIRF microscopy and machine learning segmentation has students up all night.

We are getting serious about analysis. Today Hunter Elliott and @florianjug.bsky.social introduce machine learning and AI. It’s not magic, it’s computers.

Late night coding and microscopy today!

A tall bearded man with a hat and glasses stands beneath a slide at the front of a room with some chairs. The slide shows images going into some cartoon pipes, with a CellProfiler logo at the top right. He's doing a very good job explaining the concepts!

Today at @qiatcshl.bsky.social , @emiglietta.bsky.social teaching the good word of CellProfiler!

Today’s lectures on live cell imaging and fluorescent proteins by @jencwaters.bsky.social and @talley.codes
Every photon counts!

Today’s lab was all about live cell microscopy.

🍻

Today @bethcimini.bsky.social and @damiandn.bsky.social lectured on image correction and colocalization. Key take-home: The computer can’t do magic; if you don’t collect photons at the microscope you can’t make them appear later.

Today Hunter Elliott is lecturing on image processing. Key take-home before lunch: There is no free lunch:)

@talley.codes teaching about digital cameras. Remember Airy disks?

Learning about the components of a microscope. How are images formed and what is that back focal plane everyone seems so excited about?

Cleaning objectives is a very important skill to learn! @fedegasparoli.bsky.social showing how it’s done:)

The famous ‚fluorescence depends on the environment‘ lecture! Need more light? Consider adding base to your sample:)

We are back! @jencwaters.bsky.social giving the first lecture of 2026 QI at CSHL. Two weeks of intense microscopy training ahead.

Apply to join #BoBiAC2026 this summer! You'll learn how to use Python to segment fluorescence images w/tools like @ilastik-team.bsky.social & CellposeSAM, & even learn how to measure cell features, neighborhood relationships, & colocalization. No coding experience needed! More info: bobiac.github.io

Apply to join us this July for a crash course on Python & bioimage analysis tailored specifically for beginners! Applications due May 18th 🔬💻 #BoBiAC2026 bobiac.github.io

This QI course is the perfect way for grad students & postdocs to strengthen fundamental knowledge and confidence in their quantitative light microscopy experimentation! Deadline to apply is TOMORROW! 🔬

Don't let the price of QI deter you!
QI offers financial aid through NCI, HHMS, and @bioimagingna.bsky.social.
Include a brief statement of need with your application.
Aid is distributed by CSHL (not the course instructors).
Many of our students - sometimes all! - receive significant support.

QI Class of 2025! Centroid = @sciencedoodles.bsky.social & @florianjug.bsky.social / first minima = @talley.codes & me.

Applications for 2026 due on Friday!

meetings.cshl.edu/courses.aspx...

There's exciting Microtutor news in store for 2026! Be the first to hear the latest on course releases and other learning opportunities by signing up for the Microtutor mailing list: lp.constantcontactpages.com/sl/5BYgJu6/m...

Quantitative Imaging: From Acquisition to Analysis Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.

🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)!

An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools.

🗓️ Apply online by Jan 30, 2026

Do you like building novel optical microscopes to answer complex biological questions? How about doing that as part of one of the world’s most unique and vibrant research and education communities, which is incidentally located in a beautiful seaside New England village? 🐙🌊🔬

These workshops are perfect for grad students & postdocs in the Boston area that are either planning or actively conducting microscopy experiments in their research! More info about the workshops, along with the application, here: cite.hms.harvard.edu/education/wo...