Designer indicators for two-photon recording of subthreshold voltage dynamics - Nature Methods The genetically encoded voltage indicators JEDI3sub and JEDI3hyp enhance monitoring of subthreshold neuronal activity in the awake mouse brain using a variety of two-photon microscopy techniques.

New voltage indicators JEDI3sub and JEDI3hyp allow monitoring subthreshold neuronal activity in awake mice. @stpierrelab.bsky.social

www.nature.com/articles/s41...

If you are a PhD student or post-doc who wants to learn everything about #electrophysiology or #imaging, consider registering to the Paris Spring school in optical imaging and electrophysiology, that will take place in the center of Paris 11-23 May 2026. Application deadline Feb 2nd
parisneuro.ovh

Glutamate indicators with increased sensitivity and tailored deactivation rates - Nature Methods iGluSnFR4f and iGluSnFR4s are the latest generation of genetically encoded glutamate sensors. They are advantageous for detecting rapid dynamics and large population activity, respectively, as demonst...

iGluSnFR4 is now available @natmethods.nature.com

www.nature.com/articles/s41...

Glutamate indicators with increased sensitivity and tailored deactivation rates 🧪

We are excited to announce that @karthala-system.bsky.social has been acquired by Imagine Optic.

More details available in the press release below :

🧵 New paper out🚨🧠! Information about motion is key to vision. Head motion signals from the vestibular system robustly modulate visual cortex activity. Despite this profound modulation, we still don’t know how these signals reach visual cortex - or even what aspects of head motion are transmitted.

Mind the deadlines for our 11th international conference "BrainBody - Exploring bidirectional crosstalk between the central nervous system and the body"!
⏳ Early bird registration and abstract submission deadline: July 1st!
📅 1-3 October 2025
📍 Bordeaux, France

👉 brainconf.u-bordeaux.fr/en/

Introducing warpfield, an open source Python library for GPU-accelerated non-rigid 3D registration. Warps and aligns gigavoxel volumes within seconds (not hours). For 3D microscopy, region-to-region and cell-to-cell matching.
A collaboration with @mh123.bsky.social 🚀
github.com/danionella/w...

(Plz repost)

I’ve been receiving some good news lately and will be hiring at all levels to expand the lab. Please get in contact if you are interested in reinforcement learning, neural plasticity, neural circuit dynamics, and/or hearing rehabilitation.

pierre.apostolides @ umich .edu

Tyvm

✨ Announcing a new glutamate indicator - iGluSnFR4! ✨

Launched as a pair, iGLuSnFR4s and iGluSnFR4f have high-sensitivity and fast activation/deactivation for recording synapses.

More on the indicators and what they are already revealing: www.biorxiv.org/content/10.1...

🧠📈

Standardized measurements for monitoring and comparing multiphoton microscope systems Nature Protocols - A set of procedures is described here for standardizing the evaluation of multiphoton microscope performance, covering laser power, pulse width optimizations, field of view,...

That was a fun project! Thanks to Rob Lees, Adam Packer, and all the co-authors for inviting me to participate.
rdcu.be/edQXi

Three images with figure excerpts from the paper, highlighting 1) single-spike detection analysis, 2) autocalibration analysis, and 3) non-linearity of calcium-to-fluorescence transfer functions and their effect on the reporting of complex spiking events.

Are you using GCaMP8 or are planning to switch from GCaMP6? Then check out this new preprint from our lab! www.biorxiv.org/content/10.1...

- Calcium imaging
- GCaMP8 vs. GCaMP6
- Spike inference
- Imaging + ephys ground truth

With F. Helmchen, K. Svoboda, M. Rozsa & X. Fang.

#SaveTheDate📢 Rdv for the 7th International Frontiers in Neurophotonics 2025 #FINS2025!

On the programme: experts in #microscopy and #neuroscience🔬🧠

🗓️From 6 to 9 October
📍Bordeaux (France)🍷
📝 Registration opens soon

frontiersneurophotonics.org

Save the date !
Frontiers in Neurophotonics Symposium 2025 in Bordeaux
October 6-9
frontiersneurophotonics.org/2025-speakers/
🧪

#MiFoBio2025

The autumn microscopy school by @gdrimabio.bsky.social will take place from 10th to 16th October 2025.

The call for workshops to share pratical knowledges is open till 31/01 ateliers-mifobio.fr

We'll bring stuff as usual, stay tuned !

More information 👉 linktr.ee/gdrimabio

Seems like there is a new ultracompact #femtosecond #laser source for #multiphoton #microscopy in town! 50 MHz rep rate means a 2-3 m cavity if there is a single pulse circulating in the cavity - folding that into that compact of a form factor is quite impressive!
www.laseratwork.com/products-ove...

An Open-Source Joystick Platform for Investigating Forelimb Motor Control, Auditory-Motor Integration, and Value-Based Decision-Making in Head-Fixed Mice www.biorxiv.org/content/10.1101/2025.01....

The AODscopeHUB! It makes the two-photon imaging system as versatile as you need: double the imaging setup, while using the same scanner and detection system! An ideal solution for an imaging facility and multi-user environment

Parallel mechanisms signal a hierarchy of sequence structure violations in the auditory cortex Responses to local and global violations in sound sequences are prediction errors and not simply the consequence of stimulus-specific adaptation.

Catching up from the dead time w/o X, here is the excellent recently published study by Sara Jamali with @standehaene.bsky.social using the local-global prediction paradigm in mice. Oddball signaling is not just adaptation. Sequence chunking responses in VIPs. And some more doi.org/10.7554/eLif...

Interested in understanding how things work? In particular the tools you use to study the brain? Join us at TENSS 2025 where we brainstorm ideas, build and debug microscopes, electrophysiology and behavior rigs amidst the picturesque Transylvanian hills! tenss.ro
Apply by: February 16th!

Applications to the 2025 Paris Spring School in Optical Imaging and Electrophysiological Recording in Neuroscience (AKA the Paris Neuro Course) are now open. Deadline on the 1st of February.

The course will run 14-27 May 2025.

parisneuro.ovh