Protein-templated synthesis of dinucleotide repeat DNA by an antiphage reverse transcriptase Defense-associated reverse transcriptases (DRTs) are widespread bacterial anti-phage systems that use unconventional mechanisms of polynucleotide synthesis. We show that DRT3, which comprises two dist...

👉 Protein-templated synthesis of dinucleotide repeat DNA by an antiphage reverse transcriptase:

| Science www.science.org/doi/10.1126/...

👍👍👍

A 5-Enolpyruvylshikimate 3-Phosphate Synthase Functions as a Transcriptional Repressor in Populus An isoform of the EPSP synthase enzyme possesses a DNA-binding helix-turn-helix motif and has dual roles in shikimate biosynthesis and transcriptional repr

A moonlighting (trigger) EPSP synthase in Populus trichocarpa:

5-Enolpyruvylshikimate 3-Phosphate Synthase Functions as a Transcriptional Repressor in Populus:

academic.oup.com/plcell/artic...

#triggi #glyphosate

Glutamate metabolism in Bacillus subtilis: gene expression and enzyme activities evolved to avoid futile cycles and to allow rapid responses to perturbations of the system - PubMed Glutamate is a central metabolite in all organisms since it provides the link between carbon and nitrogen metabolism. In Bacillus subtilis, glutamate is synthesized exclusively by the glutamate syntha...

B. subtilis can only utilize glutamate when either rocG or gudB1 encoding glutamate-degrading GDS are overproduced!

pubmed.ncbi.nlm.nih.gov/18326565/

pubmed.ncbi.nlm.nih.gov/31649652/

Heterologous expression of Vibrio natriegens transporters enhances the growth of Escherichia coli and Bacillus subtilis on glutamate Bacterial growth modulation is crucial in microbial synthetic biology. In this study, we found that glutamate is an extremely poor carbon source for E…

This is weird! How can B. subtilis 168 grow with glutamate as a single source of carbon and nitrogen? The cryptic glutamate dehydrogenase GudB is never mentioned.

V. natriegens transporter enhance E. coli and B.subtilis growth on glutamate:

www.sciencedirect.com/science/arti...

#subtiwiki

Initiation of swarming in Bacillus subtilis as a resolution for the sorites paradox in bacteria | PNAS As a single cell grows and multiplies, at what stage does it constitute a swarm? Here, we provide an answer for Bacillus subtilis. We place single ...

👉 Initiation of swarming in Bacillus subtilis as a resolution for the sorites paradox in bacteria:

www.pnas.org/doi/10.1073/...

@pnas.org #subtiWiki

#ParadoxieDesHaufens

de.wikipedia.org/wiki/Paradox...

😱 Wow: Competence-mediated DNA uptake diversifies Vibrio cholerae sedentary chromosomal integrons:

👇

www.science.org/doi/10.1126/...

@science.org #vibrio

Nicht vergessen! Save the date!

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Translation control by altered start codon usage as a means of modulating the general stress response and virulence in Listeria monocytogenes Author summary The general stress response (GSR) in foodborne pathogen Listeria monocytogenes is important for environmental stress response and for host entry, but GSR is also highly variable across ...

Translation control by altered start codon usage as a means of modulating the general stress response and virulence in Listeria monocytogenes:

👇

dx.plos.org/10.1371/jour...

More than deserved! Congratulations @c-elfmann.bsky.social & @joergstuelke.bsky.social!

Bacterial Schlafen proteins mediate phage defence - Nature Microbiology This study highlights that Schlafens are ancient, mechanistically conserved immune effectors that mediate antiviral immunity in organisms ranging from humans to bacteria.

🫱 Bacterial Schlafen proteins mediate phage defence:

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www.nature.com/articles/s41...

#schlafen

🫴 The membrane-bound nuclease SNIPE directly cleaves phage DNA during genome injection:

www.nature.com/articles/s41...

@spp2330.bsky.social

Phages communicate across species to shape microbial ecosystems Gallego-del-Sol et al. show that arbitrium-coding phages can sense non-cognate peptide signals from other phages to regulate lysis-lysogeny decisions. This crosstalk affects lysis-lysogeny outcomes of...

🫴 Phages communicate across species to shape microbial ecosystems:

Cell www.cell.com/cell/fulltex...

Enabling the prediction of phage receptor specificity from genome data:

👇

www.biorxiv.org/content/10.6...

AexB is an aromatic amino acid exporter that functions as a metabolic safety valve | mBio Identification and characterization of amino acid exporters is a broadly relevant topic. Amino acid synthesis is energetically costly, and thus functional relevance for their export is unintuitive. Id...

🫴 Very interesting paper: AexB is an aromatic amino acid exporter that functions as a metabolic safety valve:

| mBio journals.asm.org/doi/10.1128/...

#subtiwiki

🤔😬🫣

A family of endonucleases blocks nanotube-mediated plasmid exchange - Nature Microbiology The YokF endonuclease provides a donor-controlled mechanism to limit plasmid transfer to recipient bacteria via nanotubes in Bacillus subtilis, with YokF functional homologues found across Gram-positi...

🫱 A family of endonucleases blocks nanotube-mediated plasmid exchange: Is the flagellar type III export apparatus FlhA part of the mysterious nanotubes? 😜

www.nature.com/articles/s41...

@subtiwiki #subtiwiki

Senat der #UniHohenheim bestätigt drei Prorektor:innen im Amt: Prof. Dr. Sebastian Hess (Studium und Lehre), Prof. Dr. Julia Fritz-Steuber (Forschung) und Prof. Dr. Andreas Pyka (Internationalisierung). t1p.de/9j09v

Temperate phage evolve to integrate host stress and quorum signals in lysis–lysogeny decisions Temperate phage face conflicting signals from host stress and quorum sensing when deciding between lysis and lysogeny. This study shows that bacteriophage integrate information on host condition via S...

👉 Temperate phage evolve to integrate host stress and quorum signals in lysis–lysogeny decisions:

dx.plos.org/10.1371/jour...

#subtiwiki

The Ti Plasmid‐Encoded VirJ Functions as a Lysyl‐Phosphatidylglycerol Hydrolase in Agrobacterium tumefaciens We identify the long-known virulence factor VirJ as a lysyl-phosphatidylglycerol (L-PG) hydrolase. Like the chromosomally encoded virulence factor AcvB, it prevents excessive accumulation of L-PG in ...

🦠🧫 The Ti Plasmid-Encoded VirJ Functions as a Lysyl-Phosphatidylglycerol Hydrolase in Agrobacterium tumefaciens:

👇

@molecularmicro.bsky.social

onlinelibrary.wiley.com/doi/10.1111/...

Genetic underpinnings of chills from art and music Author summary Many people experience chills when listening to music, reading poetry, or viewing art. Yet not everyone feels these reactions in the same way. These differences provide a window into ho...

🎶 Genetic underpinnings of chills from art and music:

👇

dx.plos.org/10.1371/jour...

Post-translational modifications of GlmR integrate metabolic and stress signals to maintain cell envelope homeostasis in Bacillus subtilis Author summary Bacteria must integrate feedback from multiple metabolic processes and environmental cues to efficiently allocate carbon to produce essential building blocks such as nucleotides, amino ...

🫵 Post-translational modifications of GlmR integrate metabolic and stress signals to maintain cell envelope homeostasis in Bacillus subtilis:

👇👇👇

dx.plos.org/10.1371/jour...

@cdiAMP #subtiwiki

An ancestral glutamate receptor mediates cell volume regulation during high K+ stress in cyanobacteria Cyanobacteria, able to survive photoautotrophically in harsh environments, possess various ancestral homologs of ion transport systems found in eukaryotic cells. The model cyanobacterium, Synechocysti...

WOW: An ancestral GLUTAMATE receptor mediates cell volume regulation during high K+ stress in cyanobacteria! 🤔

In: The Journal of Biological Chemistry:

www.jbc.org/article/S002...

#cyanobacteria

Structure-function analysis of the bacterial ClpE/ClpP AAA+ protease General and regulatory proteolysis in bacteria is executed by a set of ATP-dependent proteases composed of hexameric ring-forming AAA+ proteins and associated peptidase barrels (e.g. ClpP). These AAA+...

🫴 Structure-function analysis of the bacterial ClpE/ClpP AAA+ protease - In: Journal of Biological Chemistry

👇👇👇

www.jbc.org/article/S002...

YqeK governs the acquisition and removal of nucleoside tetraphosphate caps in Firmicutes Abstract. Nucleoside tetraphosphate (Np4) caps have heretofore been reported in only one bacterial species: the model Gram-negative organism Escherichia co

👉 YqeK governs the acquisition and removal of nucleoside tetraphosphate caps in Firmicutes:

academic.oup.com/nar/article/...

@narjournal.bsky.social #subtiwiki

👉 Impressions from the fantastic VAAM 2026 in Berlin: