Started this project years ago, but now everything is deposited on @addgene.bsky.social and a short @protocolsio.bsky.social is online. Try it out to make your own (cheap) homemade nuclease/Benzonase!

dx.doi.org/10.17504/protocols.io.261ged7xov47/v2

Thank you @jcoker10.bsky.social and Michael Lim!

Filtered tips are a MUST while you handle it haha…it’s the most active recombinant protein I’ve ever handled.

Share your reagents, it makes science more fun!!

MCL1 may not mediate chemoresistance - preLights MCL1, an anti-apoptotic BCL-2 family protein, does not mediate chemotherapy resistance in cancer cells, which calls into question the role of MCL in therapy response.

What's the role of MCL1 in chemoresistance?

A new preLight from @kanishka03.bsky.social highlighting a #preprint from Kylin Emhoff, @jcoker10.bsky.social & Jan Joseph Melenhorst challenging the concept that MCL1 promotes chemoresistance from the nucleus.
prelights.biologists.com/highlights/m...

Opinion | AMA president: NIH is wrong to pivot from mRNA vaccines Trump once oversaw historic mRNA vaccine development. Why is he abandoning it?

My Letter to the Editor in the Washington Post is online

[Gift Link]

wapo.st/415HpYM

1/4

The Continuing Crisis, Part X: Reductions in Force, Gains in Centralized Power

The news across HHS today is awful, and the stated reasons behind it all are lies:

White House says no one will be fired over the Signal scandal because no one involved was trying to cure cancer.

graph of NIH basisfor new drugs

A pie graph worth keeping in mind as the NIH budget plummets jamanetwork.com/journals/jam... for 356 new FDA drugs approved

Things remain exceedingly bleak but the first sunny, 70 degree day of 2025 is here and it is beautiful

Trump 2.0: an assault on science anywhere is an assault on science everywhere US President Donald Trump is taking a wrecking ball to science and to international institutions. The global research community must take a stand against these attacks.

US President Donald Trump is taking a wrecking ball to science and to international institutions. The global research community must take a stand against these attacks.

https://go.nature.com/4kd1vIu

DOGE released data about federal contract savings. It doesn't add up A new government tracker claims DOGE has saved billions from ending federal contracts. But an NPR analysis of the data finds the claimed savings don't add up.

I've read most of the analyses of the DOGE 2025 "receipts" site. This, from NPR, is the best I've read so far. Some of what they claim to have cut haven't been cut, other "cuts" will incur costs down the road.

NPR says DOGE has made $2B of cuts, not $55B.

www.npr.org/2025/02/19/n...

Graphic from United for Medical Research showing that NIH funding produces $2.46 in economic activity for every $1.00 awarded

Graphic from United for Research showing that NIH funding produced over $92B in economic activity in FY 2023

Graphic from United for Research showing that $914M in NIH grants to Florida institutions supported 14,688 jobs and produced $2.78B in economic activity

Cuts to the NIH are a strategy to strangle research universities not “reduce waste”

The Browns righteously deserve their ongoing punishment for abandoning Baker Mayfield

I fell in love with basketball when I was eight because of LeBron. Tomorrow I turn 30 and he’s still doing this. Preposterous and wonderful.

I joined 75+ @nobelprize.bsky.social laureates urging US Senators to oppose RFK Jr.'s confirmation as DHHS Secretary. If you’re in a state with GOP senators, PLEASE reach out to them! I’d deeply appreciate it if you amplified this post! 🙏
www.nytimes.com/2024/12/09/h...

An all-time footballing legend and the greatest goalkeeper in the history of the USWNT #ThankYouAlyssa

It might be cold and raining but it’s a beautiful day because City has been properly embarrassed at home 🔥

Wicked goes so hard 🤯

Science is neither red nor blue Long before the 5 November US presidential election, I had become ever more concerned that science has fallen victim to the same political divisiveness tearing at the seams of American society. This i...

Science has values, and they are inherently in opposition to political ideologies centered on the rejection of evidence, the promotion of scientific falsehoods, and that seek to undermine the institutions that support scientific inquiry. You don't get to pick!
www.science.org/doi/10.1126/...