Finally, cells from patient with laminopathies exhibit very abnormal nuclear deformation in microgrooves.
This opens to the possibility of using this system as a tool to detect pathological abnormalities in nuclear mechanics...
Surprisingly, the cytoskeleton is not mandatory for nuclear penetration inside grooves.
Cell-substrate adhesive forces are the main drivers of this process.
These nuclear deformations are dynamic: nuclei can in and out of the grooves cyclically.
Entry and exit from the grooves is associated with transient changes in perinuclear stiffness.
We observed significant 3D deformations of nuclei in microgrooves, from partial to full confinement inside a groove (caging), in various cell types.
The proportion of partial/full caging can be controled by the groove dimensions.
Our latest study on nuclear deformations in microgrooves is finally out!
doi.org/10.1002/advs...
All you need to know below 👇
My new preprint is out! We highlight here different mechanisms of contact guidance in single cells and endothelial monolayers on microgrooves. Check it out!
doi.org/10.1101/2022.1…
#STED super-resolution images of microtubules 🔴 and intermediate filaments 🟢 in HUVECs. Thanks @Abberior for allowing me to try this amazing microscope!
Looking forward to present in this amazing seminar series 👩💻 Log on on october 11th!
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Contact guidance strikes again!
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Don't miss out on two highlights associated with my last publications in @NatureComms: a nice article in @Polytechnique newsletterand a 'behind the paper' postin the Nature bioengineering community. Enjoy!
go.nature.com/3x04NqI polytechnique.edu/actualites/le-…
Oops wrong tag, out in @NatureComms of course!
And we think that this mechanism could be generalized in any borderless system where individual elements are externally oriented. To conclude, here is another example where biology successfully meets physics! A very fulfilling experience for me, thanks to all the people involved!
Then we teamed up with a physicist who built a model considering the monolayer as an active fluid. By adding in the model the constraint on cell orientation provided by the grooves, we were able to predict the emergence of the cell streams!
By studying further this phenomenon, we showed that the size of the streams can be specifically controlled by the groove depth, and that the presence of cell-cell junctions is necessary for the emergence of this pattern.
When recording the movement of monolayers of endothelial cells on the microgrooves, we were surprised to see the emergence of a very specific pattern of movement: corridors of cells moving alternatively from left-right or right-left that we called “antiparallel cell streams”.
To study this particular setting, we used microfabricated substrates composed of parallel arrays of micrometric grooves, which mimic the anisotropy often found in ECMs. Vascular endothelial cells cultured on these substrates align and elongate in the groove direction.
We know that confining cell assemblies on adhesive areas of different shapes creates very cool patterns of collective movement, but in vivo cells are often individually constrained and guided by physical topographical structures in their environment. So what happens in that case?
It’s official! My first post-doc story featuring the amazing microgrooves is out in @NatureCom 🥳🤯We described an original pattern of collective movement in unconfined monolayers of endothelial cells on microgrooved substrates. Details in the thread 🧵
rdcu.be/cNTvB
I'm so thrilled to have received a poster prize at #EMBOBuildNetworks! Microgrooves rock 🤓 It was a great meeting, big thanks to the organizers.
Homage to Michel Bornens, who passed away on March 9, 2022 at the age of 84 | EMBO reports
embopress.org/doi/full/10.15…
Very proud to see our favorite phD @ClaRamonLozano present her vessel-on-chip project at #EMBOBuildNetworks!
Great review with very nice illustrations. Well done @KYohalie!
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Very nice! Congrats to the team @GabrieleSylvain
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Very useful review!
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Basement membrane topography matters! Thanks for the highlight @focalplane_jcs
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My last piece of work is now on bioRxiv 🤩 We describe an original pattern of collective movement in unconfined monolayers of endothelial cells on microgrooved substrates. Go and check it out!
biorxiv.org/content/10.110…
I had the opportunity to discuss this very nice work with @gerardoceada recently, congrats!
x.com/XavierTrepat/s…
Our latest review is out! Great collaborative work with @ClaireDessalles - on the integration by endothelial cells of the various physical and mechanical cues present in the vasculature.
x.com/BarakatLab/sta…
I'll be presenting a poster at #EMBOlivingsys this week, on patterns of collective motion in endothelial cells, come and check it!
x.com/BarakatLab/sta…
A great introduction to the physics of collective motions, very well written and explained!
x.com/RicardAlert/st…