Very happy to see our paper on postnatal plasticity in the amygdala of juvenile swine published, with Júlia Freixes and Ester Desfilis @edesfilis.bsky.social and myself as coauthors: link.springer.com/article/10.1...
You can find the full developmental stage series on Xenbase here 👇
www.xenbase.org/xenbase/anat...
PHOTO 3: Close-up of the limb bud.
🔎 Look closely! At this stage, the forelimb buds are just starting to peek out.
PHOTO 2: Lateral view.
🔬 After months, the tadpoles are developing at different rates. The least developed specimens have currently reached stage 50.
PHOTO 1: Dorsal/Cranial view.
🐸⭐ Follow the development of Xenopus laevis embryos with me!
Read below 👇
#Xenopus #EvoDevo #DevBio #Science #Neuroscience #LabLife
✨ Life in motion ✨
💙 The beating heart of a Xenopus laevis larva at stage 46.
#Xenopus #EvoDevo #DevBio #Science #Neuroscience
Before there’s a heartbeat, there’s this little glowing heart in the neural tube 💕 Chicken neural crest cells in magenta. 📸Image by Rocío Márquez, Strobl-Mazzulla Lab @pstrobl.bsky.social #FluorescentFriday #SciValentine
You can find the full developmental stage series on Xenbase here 👇
www.xenbase.org/xenbase/anat...
At this stage, the embryos are actively feeding, with a fully developed heart.
✨ Day 7 post-fertilization — embryos are around stage 46.
🐸⭐ Follow the development of Xenopus laevis embryos with me!
Read below 👇
#Xenopus #EvoDevo #DevBio #Science #Neuroscience
New to using Jupyter notebooks for the Allen Brain Cell Atlas?
Join us on Feb. 11 for a beginner-friendly webinar focusing on installation, set-up, and common questions.
Register for the meeting link and recording: alleninstitute-org.zoom.us/webinar/register/WN_q0sJ...
You can find the full developmental stage series on Xenbase here 👇
www.xenbase.org/xenbase/anat...
✨ Day 5 post-fertilization — embryos are around stages 39-40.
🐸⭐ Follow the development of Xenopus laevis embryos with me!
Read below 👇
#Xenopus #EvoDevo #DevBio #Science #Neuroscience
You can find the full developmental stage series on Xenbase here 👇
www.xenbase.org/xenbase/anat...
Some embryos show altered development.
✨ Day 4 post-fertilization — embryos are around stages 37/38.
Obvious heartbeats 💓, visible blood flow, melanophores along the tail 🐸.
🐸⭐ Follow the development of Xenopus laevis embryos with me!
Read below 👇
#Xenopus #EvoDevo #DevBio #Science #Neuroscience
You can find the full developmental stage series on Xenbase here 👇
www.xenbase.org/xenbase/anat...
✨ Day 3 post-fertilization — embryos are around stages 25–26 and start showing their first movements.
🐸⭐ Follow the development of Xenopus laevis embryos with me!
Read below 👇
#Xenopus #EvoDevo #DevBio #Science #Neuroscience
You can find the full developmental stage series on Xenbase here 👇
www.xenbase.org/xenbase/anat...
However, embryonic development is not fully synchronized—some embryos can still be observed at blastula stages.
✨ Day 2 post-fertilization: embryos are around stages 17–18.
🐸⭐ Follow the development of Xenopus laevis embryos with me!
Read below 👇
#Xenopus #EvoDevo #DevBio #Science #Neuroscience
Fig. 1. IGF2 and Cerberus mRNAs cooperate in ectopic head induction in Xenopus embryos. Embryos were microinjected into the ventral marginal zone of a single blastomere at the 4- to 8-cell stage. (A) Uninjected control sibling at early tailbud stage (n = 81). (B) A single ventral injection of IGF2 mRNA caused a small ectopic head protrusion with a pigmented cement gland on the belly (n = 86, 68 % with ectopic structures). (C) Cerberus mRNA induced a secondary head-like structure (n = 78, 94 % with ectopic heads). (D) Co-injection of IGF2 and Cerberus mRNAs induced a large ectopic head with an expanded cement gland (n = 90, 98 % with ectopic heads). (E–H) Panoramic views of control and injected embryos. Injected mRNA doses per embryo were: Cerberus, 100 pg; IGF2, 2 ng. Results from two experiments. Scale bars are 500 μm (A-D) and 2 mm (E-H).
Fig. 6. Dominant-negative IGF receptor 1 blocked ectopic head formation by Cerberus mRNA in single ventral injections. (A) Control embryo at stage 24 injected with LacZ (100 pg) mRNA but not stained for β-galactosidase (n = 50). (B) DN-IGFR (600 pg) and LacZ injected embryos (n = 20, all normal). (C) Cerberus (100 pg) injected embryos with ectopic heads (n = 56, 96 % ectopic heads). (D) DN-IGFR blocked Cerberus ectopic heads (n = 33, 88 % with no ectopic structures, 12 % with small cement glands). (E–F) LacZ staining for (A–D). Scale bar, 500 μm.
A very curious paper from the De Robertis lab shows how Cerberus - a growth factor that inhibits Wnt signalling, and IGF - a growth factor that activates MAPK signalling, can synergistically induce a new head (aka ectopic archencephalic differentiation) in Xenopus embryos.
doi.org/10.1016/j.cd...
🚨🌿 Not everything in nature is perfect.
I’ll be documenting a recent Xenopus laevis egg laying and following the embryos through their developmental stages.
✨ Beginning with day 1 after fertilization: the very first hours of Xenopus laevis development.
From the cleavage stage through blastula and early gastrula stages.
🐸⭐ Follow the development of Xenopus laevis embryos with me!
Read below 👇
#Xenopus #EvoDevo #DevBio #Science #Neuroscience
