1) The Herzik lab is excited to announce our newest preprint in collaboration with the Grotjahn lab! We have developed a surfactant cocktail that seeks to overcome one of the more nefarious problems in #cryoEM, Air-water interface (AWI)-induced damage and preferred orientation (PO).

Fun collaboration with the Herzik lab! Very cool to see #TeamTomo + STA directly capture the effects of the AWI at the single-particle level. we know it’s happening, but it’s striking to see it so clearly!

Rubbish paper "Is Pee Stored in the Balls" with broken diagram, broken, table, and bullshit text.

So initial experiments with Open AI's vibe-coding science tool Prism are going about as well as expected.

Discovery of Non-Degradative Covalent Molecular Glues for Transcriptional Reprogramming Transcriptional reprogramming through induced proximity has emerged as a powerful strategy for modulating the expression of oncogenic and tumor-suppressive genes. Inspired by transcriptional reprogram...

Excited to highlight our latest bioRxiv led by grad student Zoe Duong and Qian Shao. We report a chemistry-centric, chemoproteomics-enabled strategy to discover non-degradative molecular glues for transcriptional rewiring.
🔗 www.biorxiv.org/content/10.6...

Leaflet-specific phospholipid imaging using genetically encoded proximity sensors - Nature Chemical Biology An approach combining bioorthogonal chemistry with genetically encoded fluorogen-activating proteins enables subcellular imaging of phospholipids and glycans, as well as the visualization of lipid tra...

Very excited to share new work out today in @natchembio.nature.com on a new approach - FACES - for selectively imaging of phospholipids and other biomolecules at spatial resolutions down to individual membrane leaflets (1/n) www.nature.com/articles/s41...

Glycopolymers stabilize protein folding and protein-protein interactions via enthalpic interactions Macromolecular crowding is ubiquitous to physiological environments, perturbing the thermodynamics and kinetics of proteins via excluded volume and nonspecific chemical interactions. While crowding ha...

Thrilled to share this manuscript from my lab where we looked at how glycopolymers that compose the glycocalyx influence protein folding and protein-protein interactions via macromolecular crowding.

TLDR: The Glycocalyx stabilizes protein folding and PPIs

www.biorxiv.org/content/10.1...

Targeted Protein Acetylation Through Chemically Induced Proximity ConspectusProtein acetylation is a pervasive and reversible post-translational modification (PTM) that impacts various protein features including stability, localization, and interactions and regulate...

Happy to share our contribution to this special issue dedicated to "Proximity-Induced Chemical Biology". We provide our take on targeted protein acetylation.

Nice work Wesley and Roy!

pubs.acs.org/doi/10.1021/...

New preprint from Daniel Du @fitzpatricklab.bsky.social shows #femtosecond light pulses can lens electron beams, promising higher contrast in #cryoET and better aberration correction in #cryoEM

tinyurl.com/257sw7j9

Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture

This could be a good time to mention we are looking for a new Lab Manager / Postdoc for the biochemistry lab with expertise protein expression, purification, and protein NMR. Details to follow.

Research Scientist - Mass spectrometry - Ferguson Lab Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

Mass spec/proteomics chempostdoc in the Ferguson lab, UCSD, La Jolla, CA

chemsky 🧪 ⚗️

jobrxiv.org/job/universi...

Structural basis for the conformational protection of nitrogenase from O2 - Nature Cryo-electron microscopy of Azotobacter vinelandii FeSII–nitrogenase reveals a core complex of molybdenum–iron proteins (MoFePs), iron proteins (FePs) and FeSII, in which FeSII mediates interactions with MoFeP and FeP to position their FeS clusters in catalytically inactive but O2-protected states.

Nature research paper: Structural basis for the conformational protection of nitrogenase from O2

https://go.nature.com/4aiD344

Awesome work from Sarah, @bcryo.bsky.social and friends in the Herzik Lab! It was cool to see this come together in such a short time.

I like the inclusion of a picture the actual pond in Fig. 1A of this paper! Very serene, it sets the stage well.

Crosslinking intermodular condensation in non-ribosomal peptide biosynthesis - Nature Structural studies of tyrocidine synthetase using site-selective crosslinking probes to link condensation domains with carrier protein substrates define key interactions and molecular mechanisms of no...

Awesome application of biorthogonal chemistry to trap non-ribosomal peptide synthetase complexes for cryo-EM, out yesterday from the Burkart Lab @ucsandiego.bsky.social

www.nature.com/articles/s41...

What do they keep in here…