A more accessible summary & the impact of the findings can be found here.
Scientists stunned by ‘fundamentally new way’ life produces DNA | Science | AAAS www.science.org/content/arti...
So cool.... A landmark finding
Protein-templated synthesis of dinucleotide repeat DNA by an antiphage reverse transcriptase | Science www.science.org/doi/10.1126/...
Oh wow!!
Really cool paper. It has been a real struggle to grow archea. Should they now be cultured with other bacteria? Also interesting to see this symbiosis.
www.cell.com/current-biol...
Nice 🧵, lovely movies. Now published advanced.onlinelibrary.wiley.com/doi/10.1002/... from the group of @lauraschaedel.bsky.social
Trainee Uma: ATG18 catalyzes a budding process that could be directly involved in regulating morphology. Thomas Pucadyil, IISER-Pune: EHD1 is an ATP dependent cutter. Dynamin is involved in retromer trafficking & differentially regulated by EHD family members. #OBMT26
Sunando Dutta, IISER-Bhopal introduces 2 roads to metastasis-single cell or collective invasion. Trainee Amrita: HGF/MET facilitates the invadopodia led charge of cell migration thru the ECM. KIF16B drives MET to invadopodia surface delivery #OBMT26
Probes to quantitatively measure membrane fluidity of intracellular organeller membranes. More fluid mitochondrial inner-membranes are more respiratory. Gaurav Singh, Sunil Laxman lab, inStem #OBMT26
Suchismita: -vely charged nanoparticles are intracellularly retained more than +vely charged NP. The intracellular compartment of +ve & -ve NPs varies as do their mobility & directionality. Content/type of NP inside vesicle may organize membrane surface compsition. Nitin Mohan lab,IIT-Kanpur #OBMT26
Coarse grain Martini molecular model of Caveolin-binding shows that Caveolin both induces and senses membrane curvature. Caveolin binding induced curvature shows that cholesterol & PS are found in the neck. Durba Sengupta, NCL #OBMT26
Clathrin heavy chains mutants associated with intellectual disability. Disease CHC mutants over-expressed in Dm ⬇️ ligand uptake on heat stress, no structural or functional defects at the pre-synapse NMJ but alters post-synaptic architecture and impaired learning. Deepa Subramanyam, NCCS #OBMT26
Allosteric activation switches of AP2. Akshay: Levels of Muniscin family members influence clathrin mediated Transferrin endocytosis. Sarvesh: Phosphorylation of AP2 via BMPK2 & AAK1 regulates clathrin-mediated endocytosis Umasankar Perunthottam lab, NCCS-DBT #OBMT26
Deep dive into how fusion is mediated by viral fusogen proteins w/ pre-fusion, extension & post-fusion confirmations. A fusion loop tryptophan that inserts in the membrane & is enough to cause membrane structure disruption. Tirumala Kumar Chowdary, NISER #OBMT26
EVs & lipid droplets both facilitate virus transmission. Immature JEV egress non-lytically via EVs from neuronal cells. Lipid droplets may be used to form multi vesicular bodies and EVs. Sourish Ghosh, IICB-CSIR #OBMT26
Intro to mechanisms by which Mycobacteria subvert phagosome maturation to evade immune response. Mohd Saleem, NISER. Student Debraj: Mycobacteria release EVs spontaneously fuse w/ host plasma membrane & changes membrane tension. #OBMT26
M. smegmatis shows hypoxia induced differential fluidity in the inner (becomes more rigid) and outer membranes. Hypoxia affects the antibiotic induced membrane permeability. Lydia Mathew, Shobhna Kapoor lab, IIT-Bombay. #OBMT26
Success of the pathogen is the reprogramming of the host cell function. Mtb induces lysosome biogenesis & redistributes them in infected cells via an Mtb lipid. Anterograde lysosome motility is enhanced by Mtb lipids. Varadha Sundaramurthy, NCBS-TIFR #OBMT26
Wingless trafficking occurs with its R where dissociation does not occur during the anterograde route. Wg is internalized together with its cargo R & dissociates in acidic endosomes. Varun Choudhury, IISER-Bhopal. Student Aendrila shows role of TRAPP complex in Wg trafficking. #OBMT26
K-leak channels are non-gated, are always open, imp resting membrane potential & the E/I ratio. Help in cognitive resilience in neurodegenerative disease. 2Pore K channels are on mitochondria & modulate mitochondrial membrane potential. Swagata Ghatak, NISER #OBMT26
Synaptic release is customized for speed and the amount of secretion & regulation may matter more for insulin/immune cell cargo. Synaptobrevin makes dimers and only a few molecules are needed for fusion, although 70 molecules are present/vesicle. Debasis Das @tifr.res.in #OBMT26
Mon1 regulates Drosophila growth through insulin signalling and in a RAB7 independent manner on neuronal morphogenesis. Mon1 acts cell non-autonomously to control post-synaptic glutamate receptors at the NMJ. Anuradha Ratnaparkhi, Agharkar Research Institute #OBMT26
Multistep entry of Influenza virus introduced by Indranil Banerjee, IISER-Mohali. Student Kajal Gupta shows pro-viral roles for TRIM proteins in viral entry via endocytosis. WASH & retromer have opposing roles in IAV endocytosis that is modulated by TRIM. #OBMT26
Syntaxin 11 deficiency impaires cell-intrinsic host defence by enhancing Coxiella & Salmonella replication in monocytes & leads to immune disorders. Nisha Singh, Sandhya Ganesan lab, IISER-Trivandrum #OBMT26
Insep/IER3IP1 loss leads to cell division defects & Rab11 vesicle accumulation in Drosophila neuroblasts/human cells. Insep interacts w/ Yip1d1, whose loss leads to cytokinesis failure & Rab11 vesicle accumulation. Sonal Jaiswal, CCMB-CSIR #OBMT26
Vimlesh Kumar IISER-Bhopal introduces the lab. Student Priyabrata: Prefoldin is imp in tracheal luminal physiology & plasticity thru the cytoskeleton. Student Srikanth: Highwire undergoes LLPS & forms condensates in endocytic mutants. #OBMT26
Cilia maintenance occurs despite dynamic ciliary composition & ciliary tubulin. Conditional PCM knockdowns after cilia formation leads to cilia defects that is reversible on restoring PCM components. Swadhin Jana @ncbsbangalore.bsky.social #OBMT26
Introduces roles of FtsZ & ring formation Ramanujam Srinivasan, NISER. Student Srijita Roy shows Odin FtsZ assembles into polymeric structures in a heterologous host & can modulate GUV membrane dynamics. #OBMT26
Centrosome is a hub for cell cycle regulation & DNA damage signaling. Mapping the functional roles of oncogenic mutations in STIL via BRCA mediated AuroraA centrosomal homeostasis. Priyanka Singh, IIT-Jodhpur #OBMT26
Viruses usurp centrosomes & subvert their function. Flavivirus helicase target to centrosomes, a minimal fragment can target JEV proteins to centrosomes. Centrosomes are proviral in JEV infection. Karthigeyan Dhanasekaran, RCB-UNESCO #OBMT26
Introduces how Stat Mech can be used to study biological systems. Sandeep Choubey, IMSc. Student Prakash Dey presents a theory-expt dialogue showing multi-state actin-binding protein model to account for actin-dynamics. #OBMT26