Morphable ‘Stitched’ Sensors for Simultaneous Spatiotemporal Tracking of Correlated Bioanalytes in Living Cells Correlated changes in molecular levels and distributions are associated with all life processes and importantly regulate key decision-making events in biology. Spatiotemporal dynamics of biomolecules are essential for functions like cell signaling, transport, immunity, and recycling, and are either affected by or cause diseases such as cancers, inflammation, and neurodegeneration. In this backdrop, the ability to catch molecules of life in action using optical imaging is extremely powerful. Simultaneous tracking of biologically correlated analytes in living cells necessitates cell-permeable, multianalyte sensors. Key criteria for achieving simultaneous, correlated tracking of multiple analytes are that sensors for these analytes should enter cells at the same time and concomitantly reach the same location where we want to detect the analytes. Separate sensors, either small-molecule- or macromolecule-based, cannot fulfill these requirements directly. We introduce morphable ‘stitched’ sensors, where fluorescent sensors for single analytes can be strategically joined via native chemical ligation (NCL) on a made-to-order basis. Morphable ‘stitched’ sensors are built from a library of single-analyte sensing units conjugated to short peptide scaffolds. The use of peptide-based scaffolds in combination with NCL allows generation of modular, biocompatible, water-soluble, and importantly cell-permeable multianalyte sensors tailored to address specific biological questions. We report five proof-of-concept multianalyte sensors created from a common single-analyte sensor library using this ‘stitching’ strategy. ‘Stitched’ sensors enable simultaneous imaging and temporal tracking of bioanalytes via time-lapse imaging. In our pilot studies we image different combinations of analytes including protons, hydrogen peroxide, and enzyme activity, in live cells, affording insights into pH- and hydrogen peroxide-dependent enzyme activity. Cellular uptake studies show that the analyte-sensing modules in ‘stitched’ sensors enter cells simultaneously and as rapidly as 5 min, exhibit synchronized uptake dynamics, and are internalized in equal proportions, resulting in a uniform distribution across the cell population. Our novel morphable ‘stitching’ platform therefore offers a universal approach toward live-cell multianalyte imaging.

Molecules of life do not act alone. To understand how life works we need to visualize multiple bio-molecules together. We have designed Morphable 'stitched' sensors that can simultaneously image multiple bio-molecules in living cells. #TIFR | JACS Au pubs.acs.org/doi/10.1021/...

Ever wish we could see chemistry happen inside living cells?

A new study by #TIFR researchers Prof. Ankona Datta & Sujit Kumar Das reports a molecular framework—ABATaRs—molecular sensors that supercharge Raman signals to visualize elusive biomolecules like H₂O₂ & Cu²⁺.

@ankonachembiol.bsky.social

5 years back we came up with an idea of 'stitching' sensors using Native Chemical Ligation for achieving Multiplexed Imaging in living cells. 'Morphable Stitched Sensors' have just been accepted in JACS Au😊

Till it is online, I am sharing a Chemrxiv version
doi.org/10.26434/che...