Here's why I do not recommend running new samples using the same unmixing matrix across multiple days. Read more on www.colibri-cytometry.com/post/stabili...

Stabilizing your unmixing Today's post pulls from a lot of previous posts on this blog to show how to effectively eliminate batch effects in your flow from an experimental side. Stabilizing the unmixing is a big part of this.

Tips today on how to achieve stable unmixing over time by using spectral reference libraries.
www.colibri-cytometry.com/post/stabili...

The peculiarities of Phenograph Phenograph is one of the more popular clustering algorithms for flow cytometry.

Do you use phenograph to analyze your cytometry data? Read today's post to learn how to avoid potential pitfalls, particularly if you're using either of the implementations in R.
www.colibri-cytometry.com/post/the-pec...

Unmixing: the tortoise beats the hare Today's tip: when running your single-color controls, take it slow.

Today's tip: when running your single-color controls, take it slow. The tortoise, as it turns out, beats the hare for spectral flow cytometry.
www.colibri-cytometry.com/post/unmixin...

Today's post is very simple--we're just looking at how to create reusable settings on the Cytek Aurora.
www.colibri-cytometry.com/post/creatin...