7. Results suggest chemical reprogramming achieves comparable rejuvenation to OSKM with more homogeneous responses and reduced cellular stress.

6. This is the first study applying the complete human-optimized chemical reprogramming protocol and directly comparing it to genetic approaches under matched experimental conditions.

5. In naturally aged primary fibroblasts, chemical reprogramming ameliorated multiple aging hallmarks without activating TERT or compromising fibroblast identity markers like COL1A1.

4. OSKM induced acute senescence during treatment, requiring a chase period to resolve. Chemical reprogramming reduced senescence during active treatment without requiring chase.

3. OSKM generated heterogeneous populations: some cells retained CD13-high fibroblast identity while others acquired the TRA-1-60 pluripotency marker. Chemical reprogramming produced uniform CD13-low populations without detectable TRA-1-60.

2. Both methods reduced senescence, mitochondrial ROS, and age-associated gene expression. Chemical reprogramming matched or exceeded OSKM efficacy on most markers.

1. We compared the latest human-optimized chemical reprogramming protocol against doxycycline-inducible OSKM in progerin-aged fibroblasts and primary fibroblasts from donors >80 years old.

Rejuvenation capacity of genetic versus human-optimized chemical reprogramming in cellular aging models Cellular reprogramming with transient OSKM expression can reverse aging phenotypes, but genetic factor delivery introduces heterogeneous expression, reprogramming-associated stress, and barriers for t...

www.biorxiv.org/content/10.1...

New preprint from our lab: "Rejuvenation capacity of genetic versus human-optimized chemical reprogramming in cellular aging models"
Performed by my talented graduate student @omercane.bsky.social

7. These results suggest chemical reprogramming achieves comparable rejuvenation to OSKM with more homogeneous responses and reduced cellular stress.

6. This is the first study applying the complete human-optimized chemical reprogramming protocol and directly comparing it to genetic approaches under matched experimental conditions.

5. In naturally old primary fibroblasts, chemical reprogramming ameliorated multiple aging hallmarks without activating TERT or compromising fibroblast identity markers like COL1A1.

4. OSKM induced acute senescence during treatment, requiring a chase period to resolve. Chemical reprogramming reduced senescence during active treatment without requiring chase.

3. OSKM generated heterogeneous populations: some cells retained CD13-high fibroblast identity while others acquired the TRA-1-60 pluripotency marker. Chemical reprogramming produced uniform CD13-low populations without detectable TRA-1-60.

2. Both methods reduced senescence, mitochondrial ROS, and age-associated gene expression. Chemical reprogramming matched or exceeded OSKM efficacy on most markers.

1. We compared the latest human-optimized chemical reprogramming protocol against doxycycline-inducible OSKM in progerin-aged fibroblasts and primary fibroblasts from donors >80 years old.

A chromatin-focused CRISPR screen identifies USP22 as a barrier to somatic cell reprogramming Communications Biology - Ubiquitin-specific peptidase 22 (USP22) is identified as a key chromatin-based barrier to human iPSC derivation through a chromatin-focused CRISPR-Cas9 screen.

Check our recent work on a CRISPR screen to identify regulators of human somatic cell reprogramming where we identify USP22 as an important factor. Now published @CommsBio. rdcu.be/ed1l6

Advert detailing a postdoctoral scientist position at EMBL Rome. Contact: jamie.hackett@embl.it

Postdoc positions in epigenetics!

We have several opportunities to investigate big picture questions on (epi)genome function & mechanisms

Based @embl.org near Rome (Italy), we offer full scientific support/facilities, training & funding. Also...☀️🏛️🍕🏖️

See👇or contact me for other options. Pls RS

A screenshot of a paper on bioarxiv illustrating the lack of blue sky share button!

Would you like to see @biorxivpreprint.bsky.social add a share to blue sky button?! I know I would! Share this post to let @richardsever.bsky.social @erictopol.bsky.social and others at bioarxiv know!

It’s all a big mess. And the word for spice in turkish is baharat

Looks good!