Since the preprint version, we have updated the manuscript with additional data/discussion on the cancer selectivity of the signal and on the mutations that were important for enzyme evolution. Congrats to Wang-san and Kiji-kun! bsky.app/profile/ryos...

日本語プレスリリースはこちらです: 生体直交性プローブと改変酵素による高精度ながん蛍光可視化の実現 ~背景ノイズを低減し、高いコントラストでのがん部位特異的検出に成功~ www.u-tokyo.ac.jp/content/4002...

Low-Background Cancer Imaging with a Bioorthogonal Fluorescence Probe and Engineered Reporter Enzyme Bearing a Targeting Moiety Combinatorial use of an antibody–reporter enzyme conjugate and a fluorescence probe activated by the enzyme is a powerful strategy for fluorescence-guided cancer surgery. However, conventional probes ...

Our new paper is out in @jacs.acspublications.org
! We developed a novel pair of bioorthogonal fluorescence probe and engineered reporter enzyme, enabling low-background, bright cancer imaging in vivo. pubs.acs.org/doi/10.1021/...

日本語プレスリリースはこちらです: www.u-tokyo.ac.jp/content/4002...

Glomerular routing of tumor-derived extracellular vesicles substantiates urinary biopsy Tumor-derived extracellular vesicles cross the kidney filter into urine via glomerular transcytosis, enabling urinary diagnostics.

Our collaborative study with Yasui lab @sciencetokyo.bsky.social is out in Science Advances ! With gRNA/GeNL tagging, we showed that cancer-derived small EVs can reach and be concentrated in urine via transcytosis, providing rationale for urinary liquid biopsy. www.science.org/doi/10.1126/...

Related post in Japanese
web-cdn.bsky.app/profile/ryos...

Related post
web-cdn.bsky.app/profile/ryos...

Our paper on the antigen fluorogenic sensor is featured as a supplementary cover in this week’s @jacs.acspublications.org
! Beautiful artwork by our co-author, Ryo Tachibana! Read it here: pubs.acs.org/doi/full/10....
#MyACSCover

Thrilled our PANCS-binder tech is finally out in print!
www.nature.com/articles/s41...

This technology, 11-years in the making, has transformed how we do research in my lab, and was led by an amazing postdoc, Matt Styles, who is currently entering the academic job market - so look out for him! 1/n

Our latest paper is finally published in @natcomms.nature.com (doi.org/10.1038/s414...) !! We successfully constructed synthetic multinuclear metal complexes using only proteins.

抗原に結合するだけで光る蛍光プローブの新規設計法に関する論文がJACSで公開になりました! 日本語プレスリリースはこちらです。@jacs.acspublications.org www.u-tokyo.ac.jp/content/4002...

From the preprint version, we updated validation/demonstration of our probes, including the possilibty of lighting up antigen positive cells even in mixed cell population. Congrats to Nakadate-san, Seike-kun, and co-workers!
bsky.app/profile/ryos...
chemrxiv.org/engage/chemr...

Design of Antigen-Targeting Fluorogenic Probes Utilizing Intramolecular Addition Reaction of Protein-Dye Hybrids Fluorogenic probes for antigens are useful for various purposes, including medical diagnostics and imaging, but achieving a rapid, large fluorescence increase is difficult. Here, we report a new class...

Excited to share that our new paper is out in
@jacs.acspublications.org !
We introduce a new design principle for fluorogenic antigen probes, using an intramolecular reaction between a thiol-reactive fluorophore and a cysteine engineered into a DARPin.
pubs.acs.org/doi/10.1021/...

リンクがきちんと貼れていなかったので再掲
jes1950.jp/J_MEETING/75...

5/12(月)に第75回日本電気泳動シンポジウム@ Shimadzu Tokyo Innovation Plaza (川崎殿町)を企画しております。非常に魅力的なスピーカー陣＆会場ですので、ご興味のある方はぜひご参加ください (参加者把握のため、事前登録をお願いします)。参加費無料です！https://jes1950.jp/J_MEETING/75th_sympo/75th_sympo.html

New preprint! 当ラボ卒業生の王さんを中心として、生体直交性の高い蛍光プローブ・改変レポーター酵素ペアを開発し、低バックグラウンドのがんイメージングを実現しました。新しいレポーターシステムとして, ELISA, ADEPT他、多用途に使えると考えています！

Low-Background Cancer Imaging With a Bioorthogonal Fluorescence Probe and Engineered Reporter Enzyme Bearing a Targeting Moiety Combinatorial use of an antibody-reporter enzyme conjugate and a fluorescence probe activated by the enzyme is a powerful strategy for fluorescence-guided cancer surgery. However, conventional probes ...

New preprint! In a study led by Wang-san, we developed a novel biorthogonal fluorescence probe and an engineered reporter enzyme, enabling low-background cancer imaging. This powerful pair could also be valuable for applications such as ELISA and ADEPT.
www.biorxiv.org/content/10.1...

ハイパーリンクがうまくいっていなかったので、再掲。
www.m.u-tokyo.ac.jp/news/jobs/20...

キャリアステージ/バックグラウンドがやや違っても検討できる可能性ももちろんあるので、ご興味があればまずはご連絡下さい！

当グループ(@東大医)で博士研究員を募集しています。細胞外小胞/細胞間コミュニケーションの理解や操作を可能とする合成生物学研究に興味のある方はぜひご検討ください。もちろん学振PDの応募などの相談にものれます。ご興味があればお気軽にDMください！https://www.m.u-tokyo.ac.jp/news/jobs/20250121.pdf

2025

= 45²

= (20 + 25)²

= (1 + 2 + 3 +4 + 5 + 6 + 7 + 8 + 9)²

= 1³ + 2³ + 3³ +4³ + 5³ + 6³ + 7³ + 8³ + 9³

Are you interested in studying enhancer biology/3D genome organization in my lab at the University of Tokyo? This Biohub Network program would be a great opportunity for you! Feel free to reach out if you're interested!!
www.czbiohub.org/program-ssfc...

Designer Biomolecular Chemistry - English Member Staff Assoc. Prof. (PI) : Yasunori Okamoto Assist. Prof. : – Administrative assistant : Ayaka Noda Research assistant : Tomoko Etoh Student It's you. Apply now！

📣 Our website got a fresh makeover! 🔍 Check out our updated lab website!
sites.google.com/view/y-okamo...

新作preprint @chemrxiv.bsky.social を公開しました。チオール反応性蛍光団と抗原認識タンパク質をうまく組み合わせて、抗原に結合して初めて光る蛍光センサーを開発しました。洗浄なしでのがんイメージングなどに応用可能です。卒業生の中館さんが中心となって研究を進めてくれました! bsky.app/profile/ryos...

New class of fluorogenic probes for antigens based on DARPin-silicon pyronine conjugates Fluorogenic probes for antigens are useful for various purposes, including medical diagnostics and imaging, but achieving a rapid, large fluorescence increase is difficult. Here, we report a new class...

New preprint @chemrxiv.bsky.social ! In this work led by Nakadate-san, we have developed a new type of fluorogenic sensor for an antigen that enables wash-free cancer imaging. Check out our unique probe design using a thiol-reactive fluorophore and DARPin! chemrxiv.org/engage/chemr...

日本語プレスリリース: www.u-tokyo.ac.jp/content/4002...

Labeling cell particles with barcodes Cell-to-cell communication through nanosized particles, working as messengers and carriers, can now be analyzed in a whole new way, thanks to a new method involving CRISPR gene-editing technology. The...

English press release: www.eurekalert.org/news-release...

Behind the paper story:communities.springernature.com/posts/unveiling-the-rele...

Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators - Nature Communications The release processes of small extracellular vesicles (sEVs) remain poorly understood despite their importance. Here, Kunitake et al. present a screening platform called CIBER screening that enables e...

Introducing our recent “CIBER screening” paper describing the use of CRISPR-gRNA-barcoded extracellular vesicles (EVs) for high-throughput identification of EV release regulators @naturecomms.bsky.social
www.nature.com/articles/s41...