Crystal structure and mutagenesis of a nucleic acid-binding BRCT domain in human PARP4 PARP4 is an ADP-ribosyltransferase typically associated with the cytoplasmic vault organelle. PARP4 has a distinct domain composition relative to other PARP enzymes; however, the N-terminal region of ...

Crystal structure and mutagenesis of a nucleic acid-binding BRCT domain in human PARP4 - Journal of Biological Chemistry www.jbc.org/article/S002...

PARP enzyme de novo synthesis of protein-free poly(ADP-ribose) Langelier et al. discover that PARP1, PARP2, and Tankyrase catalyze de novo synthesis of protein-free poly(ADP-ribose), or PAR, in addition to canonical protein-linked PAR. Cellular free PAR did not r...

Intriguing new paper by @mflangelier.bsky.social in the Pascal lab showing that PARP1 can generate free poly-ADP-ribose (PAR) (not attached to proteins) in vitro and in cells. In some cases PAR is attached to NAD+! #PARP #ADP-ribosylation #NAD+

www.cell.com/molecular-ce...