How genetically encoded sensors have lit up neuroscience Tools that track specific molecules in neurons have enabled researchers to probe previously unexplored aspects of neurobiology — although important caveats remain.

Got the last word in this great perspective on the state of fluorescent indicators in @nature.com by @dianakwon.bsky.social www.nature.com/articles/d41...

Thanks a lot, Luciano! Wishing you a great start as well.

Thanks so much for your kind wishes, Mike!

Thank you so much!

Thanks so much!

Thanks so much! Exciting times ahead!

Thank you so much for your kind wishes, Johannes! Let's stay in touch

Thanks a lot, Falk! Looking forward to exciting Collab in the future

Thanks so much, Biswajit

I am never under the misapprehension that my accomplishments are mine alone. To my parents and my partner, we had this journey together, and here we are today. Life has been rather kind that I have all of you. Looking forward to the years ahead!

I am grateful and would like to thank all my academic mentors till date, especially, my PhD supervisor Jörg Enderlein @joergenderlein.bsky.social and postdoc mentor Markus Sauer @sauer-lab.bsky.social Thank you for the opportunity to work with you and putting trust in me.

Happy to share that I will be starting my independent research career as a Research Group Leader at the German Cancer Research Center (DKFZ). Starting from January 2026, my group will be based at DKFZ National Center for Tumor Diseases (NCT WERA), Würzburg, Germany.

👏 Congratulation to the 🏆 winners of Student Award held by #PicoQuant during 30th anniversary of our annual workshop on Single Molecule Spectroscopy and Ultra Sensitive Analysis in the Life Sciences. #WS30
We were proud to once again support the next generation of scientists. #PicoQuantStudentAward

🚨
New Preprint!
Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons.

▶️ www.biorxiv.org/content/10.1...

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.

Great news: I received ERC Advanced grant #ERCAdG @erc.europa.eu CaptuRel! It aims at intelligent nanomaterials undergoing bioinspired cycle of capture and photorelease of bioactive molecules for sensing and controlling (bio)chemical gradients. Thanks to my Team, @cnrs.fr and @unistra.fr!

Fig. 4 Super-resolution microscopy in live and fixed cells with Rhobin. (A) Rhobin transiently binds rhodamine molecules and thereby constantly replenishes signal lost through photobleaching of dyes. (B-D) No-wash STED microscopy of live U2OS cells transiently expressing LifeAct-Rhobin2 labeled with 100 nM JFX650. (C, D) Zoom-ins of regions highlighted in (B) imaged either with confocal or STED microscopy. Scale bar: 20 μm (B) and 10 μm (C, D). (E-H) Rhobin enables timelapse STED microscopy of fast ER dynamics with minimal signal loss. U2OS stably expressing N-terminal tag fusions of SEC61B were stained with 1 μM Halo-JFX650 (HaloTag7, reHaloF) or 2 μM SiRhP (Rhobin2) and imaged at a frame rate of 0.387 fps for at least 100 time points. (F, G) Selected frames from timelapse acquisitions of Rhobin2:SEC61B labeled with SiRhP (F) or HaloTag7:SEC61B labeled with Halo-JFX650 (G). Scale bar: 2 μm. (H) Signal .CC-BY-NC 4.0 International licenseavailable under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprintthis version posted June 25, 2025.;https://doi.org/10.1101/2025.06.24.661379doi:bioRxiv preprint 22 loss during timelapse imaging. Mean ± SD signal inside cells over time for indicated constructs. (I-K) PAINT-type super-resolution microscopy with Rhobin. Rhobin2-SEC61B was transiently expressed in U2OS cells and labeled with 5 nM SiRhP after chemical fixation. Imaging under no- wash conditions and near-TIRF illumination. (I) Repeated, but transient binding of SiRhP molecules to Rhobin2 at low nanomolar concentrations can be observed as intensity bursts in intensity time traces extracted from a diffraction-limited area. See movie S3 for raw data of binding-induced blinking. (J) Sum image across 10,000 frames of an image stack, simulating a diffraction-limited image. (K). Reconstructed image from localized molecules in the full stack.

De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...

🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...

Plasma membrane labelling efficiency, internalization and partitioning of functionalized fluorescent lipids as a function of lipid structure Labeling the plasma membrane for advanced imaging remains a significant challenge. For time-lapse live cell imaging, probe internalization and photobleaching are major limitations affecting most membr...

New paper out! Would you like to fluorescently label the plasma membrane in live, fixed or permeabilized cells? I tested different lipid structures and labelling approaches to decide what makes a good membrane labelling probe for such imaging experiments!
www.biorxiv.org/content/10.1...

Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩

We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨

One-click image reconstruction in single-molecule localization microscopy via deep learning Deep neural networks have led to significant advancements in microscopy image generation and analysis. In single-molecule localization based super-resolution microscopy, neural networks are capable of...

Collaborative work with the Shechtman Lab and @heilemannlab.bsky.social.

Check it out here: www.biorxiv.org/content/10.1...

www.researchsquare.com/article/rs-6...

Thanks so much!

New Microscopy Technique Challenges Therapeutic Antibody Classification A super-resolution microscopy technique offers an unparalleled glimpse into how monoclonal antibodies bind to their targets on cancer cells to induce cell death.

Our work on lattice light-sheet TDI-DNA-PAINT and anti-CD20 immunotherapy antibodies is now featured in The Scientist magazine www.the-scientist.com. Many thanks to Sneha Khedkar for writing this fantastic piece. www.the-scientist.com/new-microsco...

🔬Happy to share our new preprint, where brightness is used to identify single molecule emission in 2D/3D, with a single camera ! Part of the PhD work of @laurent-le.bsky.social and Surabhi, great collaboration with @emmanuelfort.bsky.social from @instlangevin.bsky.social @ismolab.bsky.social #SMLM

Anna-Karin Gustavsson on LinkedIn: Rice’s Gustavsson receives NSF CAREER Award to investigate dynamics of… Very honored and grateful to receive the NSF CAREER Award! Big thanks to the National Science Foundation (NSF) and to my fantastic research team and…

Congratulations to Rice Chemistry Prof. Anna-Karin Gustavsson for receiving the NSF CAREER.
@gustavssonlab.bsky.social #NSFCAREER #RiceChemistry

Leaflet‐specific Structure and Dynamics of Solid and Polymer Supported Lipid Bilayers Polymer-supported or tethered lipid bilayers serve as versatile platforms for mimicking plasma membrane structure and dynamics, yet the impact of polymer supports on lipid bilayers remains largely un...

Happy to share our newest paper in Ang. Chem. and big thanks to @nkaredla.bsky.social, @faldalf.bsky.social and @tao-chen.bsky.social 🙏 onlinelibrary.wiley.com/doi/10.1002/...

🧪Paris Jussieu right now #standupforscience

Science, research & public health face unprecedented attacks in the U.S.

We stand with our American colleagues & support the Stand Up for Science call.

Join the global march tomorrow, incl. in Paris! 🧪✊

📍 March 7, 13:30 – Place Jussieu
🔗 More info: standupforscience.fr

#StandUpForScience

I'm thrilled to share that our review article, "Molecular Level Super-Resolution Fluorescence Imaging," has now been published online (www.annualreviews.org/content/jour...)! We provide a comprehensive overview of fluorescence super-res methods that push the limits towards molecular-scale imaging.