New #organoid research: Distinct SOX9 single-molecule dynamics characterize adult differentiation and fetal-like reprogrammed states in intestinal organoids https://ow.ly/b0H650Y1lRz

Cell Press | The Gairdner Foundation | SickKids Foundation | Universität Bayreuth

Thank you!

And thanks to Stem Cell Reports @stemcellreports.bsky.social and the ISSCR @isscr.org for highlighting our work!

Many thanks to all co-authors for their contributions!

Sathvik Ananthakrishnan @sathvik-ak.bsky.social | Gina Dailey | Anna Maurer | Claudia Cattoglio

Our work suggests that the mobility of lineage transcription factors could be used as an indicator of the cellular differentiation state.

While the fraction of chromatin-bound SOX9 molecules decreases during adult differentiation independent of SOX9 dosage, it increases upon long-term SOX9 overexpression-induced reprogramming to a fetal-like state.

Automated live-cell single-molecule tracking in enteroid monolayers reveals transcription factor dynamics probing lineage-determining function Walther et al. develop an automated live-cell single-molecule tracking and analysis pipeline allowing imaging of hundreds of cells, the capture of cellular features, and correlation of these features ...

Using our automated live-cell single-molecule tracking pipeline ( www.cell.com/cell-reports... ), we found that distinct single-molecule dynamics of SOX9 characterize cellular states in intestinal organoid models:

Thrilled to see our latest work featured on the cover of Stem Cell Reports: www.cell.com/stem-cell-re... !

Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ - Nature Communications A nanoscale 3D DNA tracing workflow visualizes Cohesin-dependent loops in single, structurally well-preserved cells. Computer simulations based on the tracing data give further insight into how Cohesi...

Our new non-denaturing nanoscale 3D DNA tracing technology provides single-cell (and sister-chromatid specific!) readout of chromatin structure with 10kb genomic resolution - Check it out!

www.nature.com/articles/s41...

Congratulations to Kai and Oyvind!!

Fantastic news! Congratulations, Florian! All the best for this new chapter!

Our new findings on how chromosomes get ready for cell division are now published in @cellpress.bsky.social!

Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩

www.sciencedirect.com/science/arti...