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Posts by Deepak Haarith, Ph.D.

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Soybean Rhg1 resistance to Heterodera glycines hinders the parasitism of Pratylenchus penetrans | Plant Disease Rhg1 has been the most effective QTL deployed in soybeans (Glycine max) to control soybean cyst nematode (Heterodera glycines; SCN). However, the resistance mechanisms and specificity of Rhg1 towards SCN as opposed to other plant parasitic nematodes are not well known. In this study we report that Rhg1 can hinder the parasitism of the root lesion nematode Pratylenchus penetrans (Pp), which is commonly found along with SCN in northern U.S. soybean fields. Two elite public soybean varieties, carrying either rhg1-a + Rhg4 or rhg1-b, negatively impacted Pp populations measured at 30 dpi. This contribution of Rhg1 was more rigorously demonstrated using near isogenic lines for rhg1-b. Additionally, in three of the four soybean genetic backgrounds tested we observed significantly more Pp at 30 dpi when Pp were co-inoculated with SCN, consistent with a previous study. No significant changes in SCN cyst numbers upon co-inoculation with Pp were observed for any of the SCN-resistant rhg1 lines. No nematode-induced changes in Rhg1 transcript abundances were observed when sampling infected root sections in any of the plant genotypes, and no changes in a jasmonate response indicator were observed. However, the 3 dpi salicylate-mediated response to SCN or Pp became more robust during SCN + Pp co-infection. Multiple hypotheses for further dissection and manipulation of soybean resistance to Pratylenchus root lesion nematodes are suggested based on the present findings.

Happy to share that my recent work on <i>Pratylenchus penetrans </i> and Soybeans is now published online as First Look. Open access article coming soon on Plant Disease @plantdisease.bsky.social @plantdiseasej.bsky.social

apsjournals.apsnet.org/doi/epdf/10....

1 month ago 1 1 0 0
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Postdoc (2 years) in plant genomics with a focus on transposable elements

🧪🌾VACANCY – Postdoc in Plant Genomics🧬

Do you work with plant molecular biology, genomics, bioinformatics or systems genetics?

Join Nathaniel Street’s group and study transposable elements in conifers!

📅 Apply by 11 February

Full details & application:
www.umu.se/en/work-with...

3 months ago 6 6 0 1
The “That's On Me, I Set the Bar Too Low” meme, but edited to reference Brassica oleracea. Text as follows:
 
Jake: I'm an expert on vegetables
Jess: Oh yeah? Name ten vegetables
Jake: Brassica oleracea
Jess: That's on me, I set the bar too low.

The “That's On Me, I Set the Bar Too Low” meme, but edited to reference Brassica oleracea. Text as follows: Jake: I'm an expert on vegetables Jess: Oh yeah? Name ten vegetables Jake: Brassica oleracea Jess: That's on me, I set the bar too low.

This isn’t actually a thread of different posts. It’s all just different cultivars of a single post

6 months ago 251 34 4 7
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NSF seeks public input on its Fiscal Year (FY) 2026–2030 NSF Strategic Plan

The NSF strategic plan FY 2026-30 is available for public comment until Jan 27. Please make your voice heard! www.nsf.gov/od/updates/n...

3 months ago 13 14 0 0
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When someone borrows your pipette without asking

3 months ago 26 5 1 0
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3 months ago 3 0 0 0
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Haarith lab end-of-semester Ice cream party!

Happy Kaarthigai, Merry Christmas, Happy Chanukah, Happy New Year, Happy Holidays!

4 months ago 5 0 0 0
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Level up! Just completed the Teacher's college at UF/IFAS CALS. Had a lot of fun and insightful discussions just ahead of teaching a large class in Spring 2026.

5 months ago 2 0 0 0
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Early birthday gift that arrived on Diwali. Very thoughtful and I love it!

#Green = The best colour in the world!
#Nematode = The best animal in the world!

Thank you, my friend Jordan Bretl! #UFBugs

6 months ago 3 0 0 0
Fig. 4.
Protein abundance immunoblots for 7 days postinoculation (dpi) samples of soybean cyst nematode (SCN)-infested root zones or similar areas from noninfested samples from same experiment as in Figure 3. The affinity-purified polyclonal antibodies raised against α-SNAPRhg1HC C-terminus peptide cross-react with the α-SNAPRhg1LC protein but not the Wm82 α-SNAPRhg1WT, whereas antibodies against α-SNAPRhg1LC C-terminus peptide are specific for the α-SNAPRhg1LC protein. Experiments used pooled samples of three roots each, separately for Set 1 and Set 2 seedlings. Similarity of total protein loading confirmed by Ponceau staining (Supplementary Fig. S4). Noncropped images for all blots shown are presented in Supplementary Figure S5.

Fig. 4. Protein abundance immunoblots for 7 days postinoculation (dpi) samples of soybean cyst nematode (SCN)-infested root zones or similar areas from noninfested samples from same experiment as in Figure 3. The affinity-purified polyclonal antibodies raised against α-SNAPRhg1HC C-terminus peptide cross-react with the α-SNAPRhg1LC protein but not the Wm82 α-SNAPRhg1WT, whereas antibodies against α-SNAPRhg1LC C-terminus peptide are specific for the α-SNAPRhg1LC protein. Experiments used pooled samples of three roots each, separately for Set 1 and Set 2 seedlings. Similarity of total protein loading confirmed by Ponceau staining (Supplementary Fig. S4). Noncropped images for all blots shown are presented in Supplementary Figure S5.

“Overexpression of α-SNAPRhg1 Can Improve rhg1-a-Mediated Soybean Resistance to Soybean Cyst Nematode,” by Deepak Haarith et al. Available open access in Phytopathology, Volume 115, Number 9: https://doi.org/10.1094/PHYTO-02-25-0077-R

6 months ago 2 2 0 0
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Differential Response of Meloidogyne enterolobii, M. floridensis, M. haplanaria, and M. incognita to Sublethal Doses of Nonfumigant Nematicides | Phytopathology® Root-knot nematodes (RKNs; Meloidogyne spp.) are among the most widespread and damaging plant-parasitic nematodes known. Although M. incognita is the most prevalent RKN species, other species, including M. enterolobii, M. floridensis, and M. haplanaria,...

Nabin Poudel et al. conducted the first comprehensive evaluation of fluazaindolizine, fluensulfone, fluopyram, and oxamyl on motility, reproduction, and egg hatching in various root-knot nematode species.🪱 Learn more: https://doi.org/10.1094/PHYTO-01-25-0028-R

6 months ago 1 1 0 0
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Soybean Rhg1 resistance to Heterodera glycines impedes the parasitism of Pratylenchus penetrans Rhg1 has been the most effective QTL deployed in soybeans to control soybean cyst nematode (Heterodera glycines; SCN). However, the resistance mechanisms and specificity of Rhg1 towards SCN as opposed...

New #soybean #nematode #pratylenchus #rhg1 preprint

www.biorxiv.org/content/10.1...

6 months ago 1 0 0 0
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Introducing the first members of Haarith Lab.

#UndergraduateResearch

7 months ago 4 0 0 0
Postdoctoral Fellow in Tomatillo Evolution

Big news! We are recruiting a postdoc to work on tomatillo evolution: jobs.colorado.edu/jobs/JobDeta.... The clade containing tomatillos and its allies (ca 300 spp) has evolved lantern fruits at least 25 times - we want to know why! Join us @rociodeanna.bsky.social & @caschenck-bio.bsky.social

8 months ago 123 108 2 3

Apparently it's Huernia aka the lifesaver cactus plant. I have been wanting it for a while. It also has rotten meat scent for the flies. Yes. I burn incense everyday in my office. So hopefully it looks pretty and doesn't smell so bad. 🤣

8 months ago 1 0 1 0
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Welcome gift - a plant? OMG YES!

Thank you Dr. Andrea Lucky for the first plant in my new office.

8 months ago 2 0 1 0
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I am looking for a PhD student in #molecularbiology #biochemistry #bioinformatics to work with #plants and #nematodes

Please share widely. Also, prospective applicants please check out this link:
www.alligator.org/article/2024...

#PhDposition

9 months ago 7 4 0 0
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My adviser organized a surprise farewell and sent me away with 5 UW Madison gifts for the 5 years I spent with him. The weather cooperated for a Terrace hangout. So long. Farewell.

9 months ago 1 0 0 0
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Overexpression of α-SNAPRhg1 Can Improve rhg1-a Mediated Soybean Resistance to Soybean Cyst Nematode | Phytopathology® The rhg1-a and rhg1-b haplotypes of the soybean Rhg1 locus are economically effective tools for the control of soybean cyst nematode (SCN; Heterodera glycines), but ongoing SCN evolution requires impr...

Overexpression of α-SNAPRhg1 Can Improve rhg1-a Mediated Soybean Resistance to Soybean Cyst Nematode | Phytopathology® apsjournals.apsnet.org/doi/10.1094/... #nematology #soybean

10 months ago 1 1 0 0
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Fun morning going through stocks and cultures as I prepare for the big move. Here is a picture of some #nematode cuties.

10 months ago 2 1 0 0
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a small white chihuahua is laying on a yellow pillow and looking at the camera . ALT: a small white chihuahua is laying on a yellow pillow and looking at the camera .

Maybe its time to get a dog :D

10 months ago 0 0 0 0
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The worst part of Spring is here: the omnipresent skunk of the tree world - Bradford Pears. Ewww

11 months ago 0 0 0 0
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Indian Grass Is NOT Browner Than Grass Abroad – AND Vice-versa (Graduate school advice) Before I begin, I want to clarify that everything I’m about to share is drawn from my own experiences and from many honest conversations I’ve had with fellow Indian students — both in person and here ...

I wrote down my learning as an international (Indian) graduate student in the USA, as a piece of advice. This could be useful to students and other academics alike? Let me know what you think! I hope this is helpful. #gradschool #newPI #international #studyabroad

www.linkedin.com/pulse/indian...

1 year ago 1 0 0 0
The impact of nematode inoculation on classical plant performance indicators. A, The effect of nematode inoculation on dry shoot biomass, dry root biomass, and root surface area at 26 days postinoculation (DPI) for each potato cyst nematode (PCN) inoculation density. The asterisks indicate significant differences (Wilcoxon rank-sum test) of the inoculated plants compared with the uninoculated control (0 eggs per gram of soil) (ns = P > 0.05, * = P < 0.05, ** = P < 0.01, and *** = P < 0.001). B, Cleaned and dried root systems of five representative plants for each inoculation density.

The impact of nematode inoculation on classical plant performance indicators. A, The effect of nematode inoculation on dry shoot biomass, dry root biomass, and root surface area at 26 days postinoculation (DPI) for each potato cyst nematode (PCN) inoculation density. The asterisks indicate significant differences (Wilcoxon rank-sum test) of the inoculated plants compared with the uninoculated control (0 eggs per gram of soil) (ns = P > 0.05, * = P < 0.05, ** = P < 0.01, and *** = P < 0.001). B, Cleaned and dried root systems of five representative plants for each inoculation density.

Robbert van Himbeeck et al. investigated whether chlorophyll fluorescence could be suitable as a rapid, nondestructive method for early potato cyst nematode detection. 🥔 Learn more: doi.org/10.1094/PHYT... @jaapjanwillig.bsky.social

1 year ago 5 1 0 0
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nematode aquatint etching from c. 2010 that i still like

1 year ago 152 21 2 0
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Very exciting Career Development workshop provided by the SON Industry & Student committees!

Registration & event information can be found here: nematologists.org/event-6040380

Hope to see you there!

1 year ago 6 5 0 0

In the American grocery stores, they label it as "Ginger root" so glad that you asked this question. 🫡

1 year ago 1 0 0 0
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Wasting a writing session on the paper's title

1 year ago 118 9 2 0
German and US flags

German and US flags

To US colleagues who have lost or are in imminent danger of losing their jobs: please DM whether we can accommodate your research.

1 year ago 308 134 3 2