Does 30mg/ml refer to 30 mg of total protein per ml, or is it the wet pellet mass per ml? I guess doesn't matter once the optimization is done. Thanks.

Yes! Mass photometry is exactly the plan. Thanks for the suggestion.

We are thinking of trying LMNG-CHS. Would love to hear advice on the Expi293 cell mass to detergent ratio for solubilization. Or protein to detergent ratio. Thank you.

Hello Colleagues. We are purifying a complex of two #membrane proteins from Expi293 cells. We used 1% DDM for solubilization, and 0.02% during chromatography. The complex shows both subunits on the gel but runs really small on SEC. It should have 3 copies of one subunit and 2 of the other.

Can you please add me? Thank you.

Can you please add me? Thank you.

Can you please add me? Thank you.

Can you please add me? Thank you.

Here is a starter pack for CryoEM/ET enthousiasts
To be completed
go.bsky.app/JmQksC

Can you please add me? Thank you.

Can you please add me? Thank you.

A single C-terminal residue controls SARS-CoV-2 spike trafficking and incorporation into VLPs - Nature Communications Dey et al. use structure-function methods to show that partial mimicry of the coatomer-binding motif in the SARS-CoV-2 spike is crucial for its release post coatomer-dependent delivery, thus ensuring ...

Hello, I am an Assistant Professor at the Univ of Maryland. We investigate #coronavirus glycoprotein trafficking, using #cryoem #cryoet #xraycrystallography and functional tools. Here is one of our no-longer-recent publications. Great to see a vibrant community!

www.nature.com/articles/s41...