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📡🤩Ultimate GCaMP Comparison⚡
GCaMP6 → jGCaMP7 → jGCaMP8 → RiboL1-jGCaMP8
We benchmark sensitivity, kinetics, soma targeting & RO-injection brightness to identify the best GECIs for each experimental need.
www.ebraincase.com/support/lite...
#GCaMP #GECI #Neuroscience #CalciumImaging #NeuralActivity
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Top left: Representative single plane confocal images showing fore-, mid-, and hindbrain regions of live zebrafish larva expressing jRGECO1a, FRCaMPi, and SomaFRCaMPi in neurons driven by the HuC promoter (n = 10 fish at 6 dpf for each). Bottom left: Schematics of looming stimulation set up in which black expanding circular shadows projected in front of the larval zebrafish embedded in agarose gel. Right: Representative single trial fluorescence traces of soma and neuropil region of red GECI-expressing neurons during looming stimulation shown as ticks.
We've had advances in green genetically encoded Ca2+ indicators (GECIs), but what about red-shifted GECIs? @piatkevich.bsky.social &co present SomaFRCaMPi, a new soma-localized red #GECI that exhibits improved accuracy & sensitivity for in vivo neuronal imaging @plosbiology.org 🧪 plos.io/3YnCqRW
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Top left: Representative single plane confocal images showing fore-, mid-, and hindbrain regions of live zebrafish larva expressing jRGECO1a, FRCaMPi, and SomaFRCaMPi in neurons driven by the HuC promoter (n = 10 fish at 6 dpf for each). Bottom left: Schematics of looming stimulation set up in which black expanding circular shadows projected in front of the larval zebrafish embedded in agarose gel. Right: Representative single trial fluorescence traces of soma and neuropil region of red GECI-expressing neurons during looming stimulation shown as ticks.
We've had advances in green genetically encoded Ca2+ indicators (GECIs), but what about red-shifted GECIs? @piatkevich.bsky.social &co present SomaFRCaMPi, a new soma-localized red #GECI that exhibits improved accuracy & sensitivity for in vivo neuronal imaging @plosbiology.org 🧪 plos.io/3YnCqRW
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Top left: Representative single plane confocal images showing fore-, mid-, and hindbrain regions of live zebrafish larva expressing jRGECO1a, FRCaMPi, and SomaFRCaMPi in neurons driven by the HuC promoter (n = 10 fish at 6 dpf for each). Bottom left: Schematics of looming stimulation set up in which black expanding circular shadows projected in front of the larval zebrafish embedded in agarose gel. Right: Representative single trial fluorescence traces of soma and neuropil region of red GECI-expressing neurons during looming stimulation shown as ticks.
We've had advances in green genetically encoded Ca2+ indicators (GECIs), but what about red-shifted GECIs? @piatkevich.bsky.social &co present SomaFRCaMPi, a new soma-localized red #GECI that exhibits improved accuracy & sensitivity for in vivo neuronal imaging @plosbiology.org 🧪 plos.io/3YnCqRW
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