A Schematic Showing a Brief Overview of the Proposed Pipeline

PyMOL Visualization showing the full icosahedral 'shell' of the MS2 bacteriophage (PDB: 2MS2).

PyMOL Visualization showing a single 'monomer' or 'building block' of the MS2 capsid protein. (Yellow is Beta Sheets, Red is Alpha helices, Green is in between loops)

Part of HTGAA Week 4 is to write a proposal on an In-silico Bacteriophage Engineering.

My proposal: 'ArmoredPhage' 🛡️

My goal: Engineer a Thermostable MS2 Bacteriophage Using Protein Design Techniques.

Check it out:
djosergenomics.github.io/Armored-Phag...

#HTGAA #synbio #AlphaFold #proteindesign

PyMOL Visualization of Structures of Tyrosinase Enzyme (Red Alpha Helices, Yellow Beta Sheets, Green In between Loops) (PDB ID: 3NM8)

PyMOL Visualization of Active Sites of Tyrosinase (Copper (II) ions in Orange) - Transparency = 0.35 (PDB ID: 3NM8)

Week 4 of HTGAA (Protein Design) is done & I'm amazed!

I was always intimidated by 3D protein models in papers & never thought I'd understand them one day!

Today, I loaded a PDB file into PyMOL, saw the alpha helices & beta sheets of my Tyrosinase enzyme.

This is so cool. 🚀
#HTGAA #proteindesign

Djoser Genomics Step Pyramid Drawn by Opentrons virtual robot

Week 3 of HTGAA is all about lab automation! 🤖

I wrote a script to program a virtual Opentrons robot to draw my Djoser Genomics pyramid. It simulates robots pipetting 100+ dots of fluorescent E.coli because manually it is slow & error-prone.

#HTGAA #synbio #Opentrons #automation #bioinformatics

I am going to audit the How to Grow Almost Anything (HTGAA) course for my first introduction to my Synthetic Biology journey.

I just did the Bio Bootcamp, & i really loved the interactive sessions on Benchling and Opentrons.

Super Excited for more!
#SyntheticBiology #Bioengineering #MIT #HTGAA