A multioanel figure showing schematics for creating mice with an Lrp5 allele that lacks the class A repeats and LRP5 protein expression in mouse embryonic fibroblasts. Panel A: CRISPR/Cas9 targeting schematic of intron 17 and 19 of mouse Lrp5. Panel B: Model of wild-type and modified LRP5 proteins on the cell surface. Panel C: LRP5 protein expression in mouse embryonic fibroblasts.
Cassandra Diegel, @bartwilliams.bsky.social & co generated mice carrying an Lrp5 allele lacking the class A repeats and observed reduced bone mass and retinal vascularisation, suggesting an essential role for these repeats in normal #LRP5 function.
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