Fig. 4. Entericglia sensitizeTRPV1+nociceptorsthrough Cx43-dependentmechanismsinvolvingIL-1β signaling.(A) Representative confocalimagefromTrpv1Cre;GCaMP5g-tdTmicecolonwhole-mountpreparationsin whichentericglia were labeledwith GFAP (yellow) and TRPV1+nociceptors(magenta)labeledwith tdTomato. (B) Schematic of the experimentalparadigm.(C) Representative imagingof baselineand capsaicin(CAP)–provoked Ca2+responsesin nerve fibersfromTrpv1Cre;GCaMP5g-tdTmice in isolated distal colonpreparations.Scalebars, 20 μm. (DtoF) Representative traces of average nerve fiberresponsesper ganglia,reportedas ΔF/Fover time (D and E), and quantification (F) of peak fluorescencevaluesof control (gray) and IL-1β–treated tissuepreparations (orange)in whichentericglia were stimulated with ADP and nociceptorswere stimulated with capsaicin(CAP). Data (F) are means± SEMfromn= average nerve responsesfrom five to seven gangliafrom three or four mice per treatmentgroup. Individualvalueswere representedas triangle

MedSky🧪 IDSky #BraingutAxis #Neurobiology #Mucosalimunology @scisignal Overview & understanding of the underlying mechanisms of Enteric glia sensitize nociceptors in visceral pain during inflammation

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