Immunofluorescence confocal micrographs (IF) from control (ctrl.) and foxi1 morphants (foxi1 MO; 3 or 0.5 pmol) at st. 32 stained for Acetylated-α-tubulin (Ac.-α-tub., cilia, gray), F-actin (Actin, cell borders and morphology, gray). Targeted cells were identified by membrane RFP expression (memRFP, magenta) and are outlined in blue. Strongly targeted areas targeted by 3 pmol of foxi1 MO fail to generate intercalating cell types. Location of insets is indicated by dashed yellow box in upper panels.
The TF Foxi1 regulates #ionocytes and is associated with several human diseases. @walenteklab.bsky.social &co show that low concentrations of Foxi1 in progenitor cells maintain #epidermal identity via the #NotchPathway, affecting #mucociliary cell fates in Xenopus @plosbiology.org 🧪 plos.io/3YLOhcp
