Some fun with DNA 3D #STED imaging. Enjoy and compare #confocal and iso-3D STED images (110 z-slices with 50 nm voxel) of living cell nucleus. #chromatin looks very nice, but we think it could be better. What do you think?
#notransfection #GOLIVE #DNA
Can it be something better than SiR-actin? Yes, it can! This is 6-610CP-JAS (yellow). If you have trouble with staining cells due to efflux read this, fresh on @biorxivpreprint : www.biorxiv.org/content/10.1101/2020.02.... #GoLive #Notransfection #gomulticolor
Sometimes you can have a hole in your DNA 😀😀😀 Enjoy 3D STED image of the nucleus with a hole in the chromatin of living cell. #GOLIVE #chromatin #notransfection #fluorescence
Living cells super-resolution imaging does not have to be expensive! See movie of living fibroblasts stained with our newest fluorogenic probes for tubulin (green) and DNA (magenta) taken on STEDYCON from @AI_ontour . Interested, contact us. #gomulticolor #GoLive #notransfection
A set of green-emitting rhodamine dyes for staining of living cells and compatible with super-resolution microscopy. #golive #gomulticolor #notransfection
Nice work from our department and Abberior chemists: onlinelibrary.wiley.com/doi/abs/10.1002/cbic.201...
Hmm... large field imaging is always attractive! It gives excellent statistics and produces more reliable results. Enjoy bird's-eye view on the living cells stained with actin and tubulin probes. #golive #gomulticolor #notransfection
Sometimes labelled cell looks beautiful and excellent performance of @AI_ontour STED microscope helps to show it! Enjoy perfect image of microtubules (yellow) and mitochondria (magenta) in living cells. #golive #gomulticolor #notransfection
Christmas is close and cells get ready: actin, talin, tubulin and mitochondria are blinking all the colors in living cells. #GoLive #gomulticolor #notransfection #Christmas
Large stokes shift (LSS) dyes challenge: see two colors with single excitation light source by eye and record the image with smartphone. GFP-talin + SiX-actin in living cells. Interested, contact us! #gomulticolor #GoLive #notransfection #smartphone
https://pubs.acs.org/doi/abs/10.1021/jacs.7b06412
A new probe for tubulin: 510R-CTX - fluorogenic, photostable and visible by eye. You can see multiple layers of tubulin networks through eyepieces without efforts.
Enjoy nice 3D image! Interested, contact us! #notransfection #golive
https://t.co/ydBqZKY1Aj
Another challenge: four color living cell imaging without SiR probes. Blue - actin, green - tubulin, yellow - endosomes, magenta - mitochondria. Interested, contact us! #gomulticolor #notransfection #GoLive
https://t.co/IvJ9XfzLcZ
https://t.co/ydBqZKY1Aj
Another day, another three-color living cell video: mitochondria, lysosome and microtubule interplay. The key players are cell-permeant large stokes shift dyes: SiX, GeX and CX. Interested, contact us! #gomulticolor #livingcells #notransfection
https://pubs.acs.org/doi/abs/10.1021/jacs.7b06412
