Top left: Chemical structures of the five most potent compounds. Bottom left: Determining the stage in Chlamydia development targeted by our best antichlamydials. Illustration of the chlamydial developmental cycle. Effect of top compounds on EB formation in HeLa cells infected with CTL2-GFP; growth inhibition after addition of top compounds (10 µM) at different points in the developmental cycle, tested in HeLa cells infected with CTL2-GFP, as measured at 48 hpi by bulk GFP fluorescence. Right: Fluorescent microscopy images corresponding to selected time points of HeLa cells infected with CTL2-GFP and treated with different concentrations of c1e, c4e or azithromycin.
#Chlamydia cause millions of infections annually, but their obligate intracellular lifestyle makes treatment difficult. This study develops a platform that identified >60 new #antichlamydial compounds, including one that inhibits bacterial #FattyAcid synthesis @plosbiology.org 🧪 plos.io/4jycCuT