Happy #MicrosCreepy season!
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#microscreepy
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I have been slacking on the #microscreepy season! Thanks for reposting!
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For night 30 of #31NightsofHalloween #MicrosCreepy, here are some amoebae squished under a jello-like pad. The flashes you see are contractile vacuole pumping events-- this organelle colllects and expels water (like bailing water out of a boat!)
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For night 28 of #31NightsOfHalloween #MicrosCreepy,
IT'S ALIVE! These are reanimated cell "ghosts," which are cells that have had their membranes/cytoplasm washed away, leaving the cytoskeleton behind. Adding ATP activates myosin motors, which causes contraction!
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31/ #31NightsOfHalloween #MicrosCreepy
no videos on bluesky, but you can watch the microscreepy finale here: vimeo.com/879997513?sh...
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25/ #31nightsofhalloween #microscreepy
Electron microscreepy of an amoeba (creepy in two orientations!)
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23/ #31nightsofhalloween #microscreepy
Here is some actin cytoskeleton staining of cells crawling into microchannels.
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22/#31nightsofhalloween #MicrosCreepy
some crystals from an old, dried-up sample before and after adding water
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21/#31nightsofhalloween #MicrosCreepy
Naegleria are crawling amoebae most of the time, but when stressed, they can grow two flagella and swim away. This cell was stained for microtubules, which form flagella and an extensive network in the cell body.
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19/ #31NightsOfHalloween #microscreepy
Some actin cytoskeletons and nuclei appearing out of nowhere...
during my OneStep fix and stain protocol
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17/ #31NightsofHalloween #MicrosCreepy
Here's a cell that looked like it wanted to divide, dispersed it's contractile vacuole network (bright tubules), then changed it's mind and condensed it's contractile vacuole network back into one organelle. Crazy stuff! 👁️
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16/ #31nightsofhalloween #MicrosCreepy
Here is a still frame of the cells that created yesterday's maximum intensity projection! The rounded cell on the left tries to divide but fails, and the one on the right gets rounder, then blebby, then divides. I wish I could share video on here!
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15/ #31NightsOfHalloween #MicrosCreepy
Making max. intensity projections of crawling and dividing cells has sufficiently creeped me out.
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13/ #31NightsOfHalloween #MicrosCreepy
Here is a max intensity projection of Naegleria amoebae from a movie taken with Interference Reflection microscopy. The bright yellow blobs correspond to contractile vacuole pumping events!
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12/ #31NightsOfHalloween #MicrosCreepy
I got to play with a pretty cool microscope today (Zeiss 980 w/ Airyscan)
purple: actin
orange: mitotic spindle
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11/ #31nightsofhalloween #microscreepy
Here is Naegleria's (cyto)skeleton in three forms.
A dividing amoeba (with mitotic microtubules, green)
An interphase amoeba (no microtubules, yes that's weird)
A flagellate (with microtubules, green)
Actin is in purple-orange in all
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9/ #31nightsofhalloween #microscreepy
This very spooky cell (imaged by my lab mate Sarah) is a fungal frog pathogen. In this part of its life cycle, it can swim around using a flagellum, or crawl on surfaces like an amoeba.
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