Cross-species comparison reveals therapeutic vulnerabilities halting glioblastoma progression - Nature Communications Understanding the growth dynamics of GBMs can help expand therapeutic options. Here, authors use a cross-species computational approach to compare GBM cells to healthy neural stem cells, identifying p...

📖 Our new pseudotime alignment tool ptalign identifies Wnt antagonist SFRP1 as central for glioblastoma cell activation, opening new avenues for patient stratification and therapeutic avenues.

🔗 lnkd.in/ees8bhNY
by @Leo Carl Foerster, Oguzhan Kaya et al.

#glioblastoma #pseudotime #neurobiology

Pseudotime trajectory created with Monocle3 of WT and tumor follicle cells labeled by pseudotime.

Here is a tiny glimpse into our recent work: a #pseudotime analysis of wildtype and tumorigenic #Drosophila follicle epithelial cells using #Monocle3. Seeing a bioinformatic prediction that supports our in vivo findings is always my favorite part of the day!

And third, some analyses are just strange. Why would you calculate #pseudotime for a population of cells that are from clearly different lineages, like neurons and microglia? Why would you calculate the change of Rack1 expression over pseudotime, but not split it by the conditions you looked at?

Uniform manifold approximation and projection Nature Reviews Methods Primers - Uniform manifold approximation and projection is a dimensionality reduction technique used to visualize and understand high-dimensional data. In this Primer, Healy...

An excellent article on UMAP ! 🖼️ rdcu.be/d0YZT

Please keep in mind that, UMAP does not preserve global topology of the data. Thus, using UMAP embeddings as direct inputs for trajectory inference methods can lead to inaccurate results.

#scRNAseq #pseudotime #UMAP #DimensionalityReduction

Happy Birthday to @Noncodarnia - the ageless wonder with #pseudotime on his side!
#ThinkYoungStayYoung