News & Views in @natchembio.nature.com : CenSpark offers a new perspective on microtubule organization—moving beyond tubulin to capture higher-order structure in live cells.

#AcademicPublishing #ResearchImpact #Imaging

www.nature.com/articles/s41...

Just published in JACS Au pubs.acs.org/doi/10.1021/...

We show that the remarkable stability of proline-derived cofactors enabling efficient DNA labeling using methyltransferases—even under harsh conditions.

Thanks to the contributors: Jonas and Rūta.

New Year, New Microscope! We just got upgraded to Abberior MIRAVA POLYSCOPE — confocal → MATRIX → STED. First test looks good, large field-of-view and nice STED images using our probes.

@abberior.rocks @stefanhelllabs.bsky.social @mpi-nat.bsky.social

#microscopy #livecell #fluorescence #bioimaging

New preprint! The remarkable stability of proline-derived cofactors opens efficient DNA labeling possibility using methyltransferases even at harsh conditions.

www.biorxiv.org/content/10.6...

Congratulations to Ruta and Jonas .

#Biochemistry #DNA #Fluorescence

Wish all of You Merry Christmas and a Happy New Year!!!

Enjoy AI-generated card for Chromatin labeling and imaging @andimaging.bsky.social

New preprint! We systematically evaluate red-shifted rhodamine-based DNA probes for in vivo imaging. Congratulations to Kamila and Shalini et al!
Job done at @mpi-nat.bsky.social and @imprsgs.bsky.social ! Read more if you want to know which isomer is the best: www.biorxiv.org/content/10.6... .

6SiR-C3-NextA is bright far-red, cell-permeable probe for live-cell selective HDAC6 imaging. Congratulations to Van-Thang Nguyen et al. @vanthang.bsky.social from @imprsgs.bsky.social ! Developed at @mpi-nat.bsky.social nat.bsky.social and published on BioRxiv www.biorxiv.org/content/10.6... .

Celebrating 2nd PhD student of the group! 🎓 Van-Thang Nguyen (@vanthang.bsky.social) from the Chromatin Labeling & Imaging group successfully defended his thesis through the @imprsgs.bsky.social program at @mpi-nat.bsky.social. A smooth and impressive defense — and his paper is coming soon… 👀📄

We have introduced a new biocompatible sulfonium-based building block for generation of non-cytotoxic covalent fluorescent probes.
The proof-of-concept paper is out in Nature Communications: rdcu.be/eUn8F
Great job done by @mauvray.bsky.social at @mpi-nat.bsky.social Enjoy reading!

Happy DNA day!!! 😀
#DNA #Chromatin #Research

The updated Team of @andimaging.bsky.social .

We will keep you posted with our newest developments.

Thrilled to share with you our work on covalent probes! By introducing a biocompatible sulfonium-based cleavable linker, we developed a series of fluorescent probes that can label covalently endogenous tubulin in various cell lines.

www.biorxiv.org/content/10.1...

#Chembio #FluorescenceFriday

Biocompatible sulfonium-based covalent probes for endogenous tubulin fluorescence nanoscopy in live and fixed cells Fluorescent probes enable the visualization of dynamic cellular processes with high precision, particularly when coupled with super-resolution imaging techniques that surpass the diffraction limit. Traditional methods include fluorescent protein fusion (e.g., GFP) or organic fluorophores linked to ligands targeting the protein of interest. However, these approaches often introduce functional disruptions or ligand-associated biological effects. Herein, we address these challenges by developing covalent fluorescent probes for endogenous tubulin, a critical cytoskeletal protein involved in processes such as cell movement, division, and biomolecule trafficking. Using well-known tubulin binder cabazitaxel and cell permeable fluorophore silicon-rhodamine—as a basis, we introduce a novel biocompatible cleavable linker containing a sulfonium center. This allowed the construction of the optimized probe , 6-SiR- o -C9-CTX , demonstrating excellent cell permeability, fluorogenic properties, and the ability to covalently label tubulin across various human cell lines. Importantly, the targeting moiety could be washed out while preserving tubulin staining, ensuring minimal disruption of tubulin function. This labeling technique is compatible with STED nanoscopy in both live and fixed cells, offering a powerful high-resolution imaging tool. ### Competing Interest Statement G.L. is a co-inventor on the patent (EP2748173B1 and US9346957B2, applicant EPFL) describing SiR and its derivatives.

We have introduced a new biocompatible sulfonium-based building block for generation of non-cytotoxic covalent fluorescent probes. The proof-of-concept paper is on BioRxiv: https://doi.org/10.1101/2025.01.27.635008 . Great job done by @MarieAuvray4 at @mpi_nat ! Enjoy reading!

Wish all of You Merry Christmas and a Happy New Year!!! Enjoy DALL-E AI-generated card for Chromatin labeling and imaging @AndImaging

Finally, we have the 1st PhD student Shalini Pradhan @ShaliniPradha19 from Chromatin labeling and imaging group from @IMPRS_GS program at @mpi_nat . It was challenging to make the full program in a single day, but we succeed.

Need to make perfect STED image? Check the primer below.

Little secret: it has useful stuff in the Supplementary Information 😉 https://x.com/MethodsPrimers/status/1821500388614254743

The updated Team of @AndImaging . We will keep you posted with our newest developments. Follow us:
@MarieAuvray4
@J_Bucevicius
@GerasRuta
@ShaliniPradha19
@GeorgijKostiuk
@Eyeshield_VN_21
@D_Bubnyte

Happy #DNADay! See how approx. two metres of DNA looks under #STORM and #STED microscopes.

The updated Team of @AndImaging . We will keep you posted with our newest developments. Follow us:
@MarieAuvray4
@J_Bucevicius
@GerasRuta
@ShaliniPradha19
@GeorgijKostiuk
@Eyeshield_VN_21
@D_Bubnyte
@Marce_Claudia_

Merry Christmas and Happy New Year!!!

KTU alumnės chemikės kelias: nuo Marijampolės iki laboratorijos Vokietijoje, kurios departamento vadovas – Nobelio premijos laureatas KTU alumnė dabar studijuoja Makso Planko institute Vokietijoje, o jos departamento vadovas – Nobelio premijos laureatas.

KTU alumnės chemikės kelias: nuo Marijampolės iki laboratorijos Vokietijoje, kurios departamento vadovas – Nobelio premijos laureatas ctf.ktu.edu/news/ktu-alumnes-chemike... #ktu

Excellent collaboration with Laura Manelytė from @uni_regensburg :

Biochemical and cellular insights into the Baz2B protein, a non-catalytic subunit of the chromatin remodeling complex academic.oup.com/nar/article/doi/10.1093/...

The updated Team of @AndImaging . We will keep you posted with our newest developments. Follow us:
@MarieAuvray4
@J_Bucevicius
@GerasRuta
@SaladinLazare
@ShaliniPradha19
@GeorgijKostiuk
@Eyeshield_VN_21 @D_Bubnyte

Rhodamine–Hoechst positional isomers for highly efficient staining of heterochromatin Hoechst conjugates to fluorescent dyes are popular DNA stains for live-cell imaging, but the relationship between their structure and performance remains elusive. This study of carboxyrhodamine–Hoechst 33258 conjugates reveals that a minimal change in the attachment point of the dye has dramatic effects on t

All probes have to be used with caution. The improved design stains DNA brighter and is less toxic: https://doi.org/10.1039/C8SC05082A https://x.com/LSAjournal/status/1704457687100981675

We are developing fluorescent probes for live-cell far-field nanoscopy imaging. Here is our team:

Perfect application of our DNA probe: https://x.com/guillermo_orsi/status/1679854382592974851

Hot question in Germany discussed by @BMBF_Bund :

How long should be the postdoctoral phase of researchers?

Have a nice weekend, and thanks for your opinion!

#WissZeitVG #AcademicTwitter #Germany #research #postdocs

Our newest review paper about sequence-specific DNA labelling for fluorescence microscopy is out in Biosensors and Bioelectronics journal: https://t.co/6fZfHtFWKi . Enjoy reading! #Biology #Bioimaging #DNA #CRISPR #TALE #labeling #Fluorescence #Microscopy #Review

A general highly efficient synthesis of biocompatible rhodamine dyes and probes for live-cell multicolor nanoscopy - Nature Communications Rhodamines are privileged fluorescent dyes for labelling intracellular structures in living cells. Here, the authors present a facile protecting-group-free synthesis permitting generation of a wide range of symmetrical and unsymmetrical 4-carboxyrhodamines covering the whole visible spectrum.

We enjoy microscopy of living cells stained with rhodamine's 4-isomers. Follow the dynamics of mitochondria (magenta), vimentin (green), tubulin (gray) and nucleus (blue). Need more information, read: https://doi.org/10.1038/s41467-023-36913-2 Happy #FluorescenceFriday and #StPatrickDay !

Finally, our paper describing more rhodamine 4-isomers and >80 probes is out in @NatureComms : https://doi.org/10.1038/s41467-023-36913-2 Enjoy a four color live-cell dynamics of nucleus (magenta), mitochondria (cyan), lysosomes (yellow) and microtubules (gray). All stained with 4-isomers.