ChimeraX-CliX now saves up to 10,000 lines of command history, and you can quickly reverse-search commands with Ctrl+R!
cxtoolshed.rbvi.ucsf.edu/apps/chimera...

Excited to share our paper about copick, a dataset API and toolkit for collaborative annotation and analysis of #cryoET data! Whether you're picking particles or curating segmentations, copick reduces friction and brings #OME-Zarr to cryoET without breaking pipelines.
🧵👇

doi.org/10.1002/pro.70578

cryoAgent: An agentic workflow for robust and adaptive end-to-end cryo-EM image processing www.biorxiv.org/content/10.64898/2026.04.16.718662v1 #cryoEM

copick: An open dataset interface and toolkit for collaborative annotation and analysis of cryo-electron tomography data pubmed.ncbi.nlm.nih.gov/41999094/ #cryoEM

VitriFlex: An Open-Source, Modular, and Customizable Robotic Platform for Cryo-EM Grid Preparation pubmed.ncbi.nlm.nih.gov/41993429/ #cryoEM

Save the date: NYC-ISB26 Integrative Structural Biology symposium - Oct 8-9, 2026

Registration coming soon.

Integrative structural biology is a powerful approach toward understanding biological macromolecular systems by bridging structural biology and biophysical methods.

nysbc.org/nyc-isb26/

A robust workflow for 3D imaging of human mitochondria using cryo-electron tomography www.biorxiv.org/content/10.64898/2026.04...

On-lamella super-resolution cryo-CLEM for cryo-ET enabled by vacuum-free ultra-stable cryogenic fluorescence microscopy www.biorxiv.org/content/10.64898/2026.04...

The next #CryoEM Current Practices Webinar speaker will be by Miao Zhang from University of Arizona speaking on "Structural basis for the pharmacological modulation of KCa2.2 channels" 4/30/2026 at 12 PM ET / 9AM PT
Registration is at no-cost, but sign-up is required: us02web.zoom.us/webinar/regi...

Thermo Fisher Scientific Cryo-EM SPA introductory mini-course (14 April, 21 April & 5 May) Learn the fundamentals of cryo-EM Single Particle Analysis (SPA) in this Cryo-EM Essentials introductory mini-course.

Part 1 was just the beginning 🚀

After the Cryo-EM SPA mini-course kickoff with @royalmicrosoc.bsky.social & ThermoFisher Scientific, don’t miss:

🗓️ Apr 21: Data Collection
🗓️ May 5: Data Processing (with Structura Biotechnology)

Register 👉 bit.ly/4cdEnrr

#cryoEM #CryoSPARC

Figure 4 from the preprint

We @embl.org wrote a short preprint about optimizing for maximum completeness in #cryoet analysis. We looked at template matching, VPP imaging, and tilt-series refinement parameters, and why averaging ribosomes helps even if you aren’t interested in their biology 🏭

www.biorxiv.org/content/10.6...

Next month, Jarrett Johnson of @schrodingerinc.bsky.social will be giving an @sbgrid.bsky.social webinar on "Upcoming improvements to visualization and CryoEM support in PyMOL”.

For more info and to sign up, see here:
instruct-eric.org/events/sbgri...

High-resolution reconstructions don’t come from “more data” – they come from better data.

Join us for Webinar 2 (Data Collection) in our Cryo-EM SPA introductory mini-course, with the Royal Microscopical Society.
🗓️ April 21, 2026 | 6 pm BST
✅ Register once for access to all sessions: ter.li/pksdqb

Now available - The Nanosoft 8-Pocket Autogrid Box - A #cryoem grid box with 8 pockets designed, shaped & sized for autogrids - Pockets are offset to make autogrid removal easier - Available in 6 colors & with a random, unique 5-digit serial number -> zurl.co/89dEH

#cryosky crowd: for a project we are looking for Quantifoil R0.6/1 copper grids that are currently unavailable from the manufacturer. If anybody has any stock to spare, please let me know! #cryoEM

Cryo-Electron Tomography Workflow for Thick Tissues Demonstrated in Mouse Hippocampus pubmed.ncbi.nlm.nih.gov/41973628/ #cryoEM

The small protein cryo-EM paper I helped start has been accepted in Nature Communications - what a relief. Thanks to @landerlab.bsky.social and @olibclarke.bsky.social for their constructive review. Congratulations to all co-authors, and wishing everyone good luck.

www.nature.com/articles/s41...

Chromatic Correction for observing biological dynamics - RFI JEOL’s pioneering chromatic aberration corrected electron microscope has at the Franklin. This is the culmination of a five-year collaboration which was started at the outset of the Franklin and has already delivered two other unique instruments. The development of the latest instrument represents the first of its type globally and the first chromatically corrected instrument in the UK. Commissioning will take place over the next year by a team at the Franklin that includes a JEOL embedded research scientist for 3 years. Initial applications of the instrument will be to use sophisticated liquid cells to study biological dynamics and to study thick samples.

Read more about this microscope here: www.rfi.ac.uk/collaborate/...

JEOL’s pioneering chromatic aberration corrected electron microscope arrived a year ago at the Franklin. This instrument represents the first of its type globally, initial applications of the instrument will be to use liquid cells to study biological dynamics and to study thick samples.

Yay proportional editing is now available in the Coot 1 API (in the daily)!

This alters the sphere of influence of dragged refinement, which helps a lot when making large adjustments - register shifts of helices, domain movements, etc.

I've added in a little widget to set it in coot1-trimmings.

Evaluating the Volta phase plate for improved tomogram alignment in cryo-electron tomography pubmed.ncbi.nlm.nih.gov/41954506/ #cryoEM

In a paper recently published in @actacrystd.iucr.org, MB&B's Jimin Wang presents two methods for detecting hydrogen atoms in high-resolution cryo-EM — visualizing what was once invisible. 🔬

Check out the article on our website: bit.ly/47Rra57

#CryoEM #StructuralBiology

It’s Not the Structure, It’s the Context It’s Not the Structure, It’s the Context on Simons Foundation

On 4/29, Alex de Marco will give a Presidential Lecture: "It’s Not the Structure, It’s the Context." He'll explore how technological advances in cryo-EM are expanding the reach of in situ structural #biology. Get tickets: www.simonsfoundation.org/event/its-not-the-struct...

In this episode we talked with @nogaleslab.bsky.social from UC Berkeley about her work on Cryo-EM and the Dynamics of TFIID and PRC2. #podcast #epigenetics #chromatin

Listen here: www.activemotif.com/podcasts-eva...

Microsoft Virtual Events Powered by Teams Microsoft Virtual Events Powered by Teams

IN JUST TWO WEEKS! April 22nd Direct Electron will be presenting a webinar: New Camera, Old Microscope - how to bring in new capabilities at a lower cost in TEM

Dr. Carter Francis and Dr. Paul Voyles will show you how you can get great results!

https://ow.ly/WBra50YCFar

Blender Rendering of vitrified HA trimer with DM at the air-water interface

We identified the maltoside detergent DM as an effective and easy solution to address preferred orientation in cryo-EM. Helped me switch from graphene grids. Special congrats to Maria Rafiq! @landerlab.bsky.social @nanigrotjahn.bsky.social @wisemanlab.bsky.social #cryoEM doi.org/10.64898/202...

The Cryo-Electron Tomography Workshop at the University of Michigan is June 7-11 - Participants will learn about standard image processing packages used to analyze cryoET data - Learn more -> zurl.co/PTRd9 Register deadline is April 10th - We are a sponsor of this event

DM: a simple solution to suppress air-water interface interactions in cryo-EM www.biorxiv.org/content/10.64898/2026.04...

2D classes of a 58 kDa particle collected on a 100 kev Tundra electron microscope

2D classes of the 58 kDa sample collected on the 100 keV Tundra TEM with a Falcon C detector at @nusbf.bsky.social @newcastleuni.bsky.social. Still much work to do to optimise the sample & data collection but its cool to see what a 100 keV TEM is capable of. #cryoEM @recon4imd.bsky.social

Developing an “ion knife” for improving electron microscopy sample preparation Instruct-ERIC is a pan-European research infrastructure in structural biology, making high-end technologies and methods available to all European researchers.

Last month, we caught up with @svenklumpe.bsky.social about research conducted by his group into “Ion Knives” for use in electron microscopy sample preparation. The work was supported by an Instruct-ERIC pilot scheme grant, and you can read more about it here: