The structure of the complete extracellular bacterial flagellum reveals the mechanism of flagellin incorporation - PubMed The bacterial flagellum is essential for motility, adhesion and colonization in pathogens such as Salmonella enterica and Campylobacter jejuni. Its extracellular structure comprises the hook, hook-fil...

pubmed.ncbi.nlm.nih.gov/40595287/

Krios is loading (precession is fun)

According to TFS this happened on several instruments after heavy/normal use

Every Krios stage is also a CNC milling machine if you operate it wrong enough #NoCryoWithoutCry

Request for tender, budget up to 75.000 €: Nextcloud server improvements Details and bid through German public tender portal: https://www.subreport-elvis.de/browseVerdingungsunterlagen.html#ELVISID:E33873881 This is just a non-binding overview. Binding are the documents i...

Call for proposals: Ernst Ruska Centre is looking for software fixes within Nextcloud. Budget is up to 75.000€
Infos in the nextcloud forum help.nextcloud.com/t/request-fo... and the german Vergabesystem: www.subreport-elvis.de/browseVerdin...

CryoSTEM workshop coming up!

Several labs use 100% ethane and 100% propane bottles and mix it during condensing stage. I tried that only once but that worked well.

If it’s vitrobot there might be a crack in the styrofoam holding the cup, allowing nitrogen to directly touch the brass.

I really like this stem stuff on a krios 🙃

Great success 🙉👍

Em101

anybody else struggling with #EMPIAR depositions today? Lots of connection and quota errors and the speed is ... german...

CryoEDS time with the material science colleagues :)

#cryoem skiing

Excited to test it against the grace hopper abomination they are currently building in Jülich

Tensorflow and relion really use the TDP limit. Most of our csparc jobs have more spikey behaviour.
It’s actually 7 gpus- not super difficult with amd epyc and server boards tbh. The 8th had problems after the blower fan customization but works now

feels absurd to use almost 4000W with one keystroke. Should we return to server GPUs in the next build? #CryoEM

Atg18 oligomer organization in assembled tubes and on lipid membrane scaffolds - Nature Communicatio... In this work, the authors determined the cryo-EM structures of autophagy-related protein 18 organized in helical tubes and provide insights into the molecular framework for the positioning of downstre...

New research from our lab doi.org/10.1038/s414...