Hello #Spectronaut users and manufacturers.. Is there any option to export both the raw and normalized PG.Quantity values?? Is it not possible at all or am I missing something here?? Thanks very much..
Computational proteomics is cool.. Even if it is not solely proteomics.. All the very best with the new role..
Link is not working for registration.. Please share a working link.. thanks..
A nature-inspired ion trap for parallel manipulation of ions on a massive scale www.science.org/doi/...
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#proteomics #prot-paper
There are a bunch of carboxylases which are naturally biotinylated and get copurified in streptavidin affinity pulldowns..
Would this protocol require removal of copurified naturally occurring biotinylated proteins as well??
I second this suggestion.. The quick and easy way to remove biotin from streptavidin is boiling in laemmli buffer.. and find a way to get rid of SDS..
Have you done an on-bead digestion?? If yes, the biotin remains attached to the bead.. The streptavidin-biotin binding is very strong.. One could use desthiobiotin which has low binding affinity and hence can be eluted out of streptavidin..
Wow!
www.nature.com/articles/s41... Here we report all five canonical nucleobases—purines (adenine and guanine) and pyrimidines (cytosine, thymine and uracil)—in samples returned from the C-type asteroid (162173) Ryugu
Can casanovo build a spectral library from de Novo search results??
Thanks.. yeah, sometimes old and tried-tested workflows are still the best bet for challenging projects..
Hybrid DIA works for making spectral library for a nontrypic peptide datasets as well??
If there is a demand for mass specs that can be operated by just clicking some buttons for predefined workflows, more models that comply with such demand will flood the market, unfortunately.. It is definitely going to improve the adoption of technology though.. Both a win and a loss for the field..
Its kind of funny that some of those very basic mass spec'ing things become challenging with these advanced models.. 😂
Setting up a decent DDA method on Astral is so mentally taxing.. It isn't meant to follow any of the data dependency criteria.. Enforcing it to follow the criteria is where it gets taxing.. Wonder how it is working for those MS2-TMT workflows.. 🤔🤔
It is an incredible honour to be featured in the Faces of Mass Spectrometry series of JASMS and share the story of a nerdy mass spectrometrist.. who despite being an introvert, always love to talk about how wonderful mass spectrometry is.. ☺️☺️
Proud to be a part of #TeamMassSpec and #FeMS.. Thanks..
Today at 13:50, on the dot, the sequences running on both Ascend and Eclipse came to an abrupt stop.. Just at random.. In the middle of a run.. Neo kept running but no MS acquisition.. Had to restart Xcalibur and reconnect LC to resume the sequence.. Very spooky!!! #MassSpecMusings
That sounds like an idea.. Thanks..
Anybody doing proteomics on FACS sorted cells? People are using BSA coated tubes to avoid cell adhesion onto the walls. BSA is all over the protein extract. How to avoid this BSA? Saw Polyvinyl alcohol (PVA) can be used in place of BSA. Any experience with its compatibility for downstream MS? Thanks
"This work provides insight to help future proteomics study designers make more informed decisions on whether physical normalization is necessary for their experiments"
Guess I am that old rigid mass spectrometrist now who finds it hard to keep an open mind to new concepts.. 😉😉
I have all these glycan specific fragments from a glycopeptide analysis.. Any software suggestions for identifying both the peptide and the attached glycan composition from such spectra?? Thanks very much.. #glycotime #TeamMassSpec
Thanks.. Have seen a few studies reporting proof of principle with MS3 strategies and MS2-MS2 ETD strategies with cleavable crosslinkers.. Any comments on that??
The interest here is exploratory where the subunits in complex are uncharacterized..
I am actually looking for the latter option.. The crosslinked peptides need to be identified by MS for confirmation.. Any suggestions?? Thanks..
#TeamMassSpec Question for XL-MS practitioners.. Looking for a suitable crosslinker to stabilise a protein complex in vitro.. What are the pros and cons for MS-cleavable vs non-cleavable cross-linkers?? Is PhoX a good choice?? Any recommendations for MS-cleavable cross-linkers?? Thanks very much..
The Spatial Omics Summer School offers a structured, hands-on introduction to spatially resolved targeted transcriptomics, proteomics, and metabolomics.
If you want to join, submit your application by 2 February 2026.
www.vibconferences.be/events/spatial-omics-sum...
King's college London
Apparently this is exactly what happened with the Lumos in my facility a few years back.. Yup, had to get it replaced..
It's almost a futile effort for people who have to go through a visa stamping process aiming to attend ASMS now.. Hopefully, the conference will be hosted in Canada again at some point..🤞🏾🤞🏾🤞🏾
(J Proteom) Two-dimensional electrophoresis-based proteomics reveals soybean seed hypocotyl proteoforms: Publication date: Available online 17 January 2026
Source: Journal of Proteomics
Author(s): Jian-Zhong Tan, Hiroyuki Kagawa, Keiko Kizawa, Hisashi Hirano #MassSpecRSS
Peptide:TMT ratio and pH are major factors influencing labeling efficiency.. Ensure both variables are controlled.. Could inject a labeled sample before pooling to quantity labeling efficiency.. Use TMT as a dynamic mod for this.. ideally up to 98% labeling on K and ~95% on N-term is anticipated..