cryoAgent: An agentic workflow for robust and adaptive end-to-end cryo-EM image processing www.biorxiv.org/content/10.64898/2026.04...

The peer reviewed version is out. Our study suggests this process is not limited to a single dimer architecture: we identified an additional dimeric arrangement, pointing to greater structural diversity in stress-induced ribosome hibernation-like states. doi.org/10.1093/nar/...

VitriFlex: An Open-Source, Modular, and Customizable Robotic Platform for Cryo-EM Grid Preparation pubmed.ncbi.nlm.nih.gov/41993429/ #cryoEM

WAT
www.science.org/doi/10.1126/...

Visualization of per-particle defocus changes; top panel shows a micrograph of the vault particle, with circular markers showing the change in defocus vs Patch CTF, with both size & color indicating the magnitude & direction of the defocus change. Outliers (>3sdev in the measured set) are indicated with *s. In the lower panel, a view looking along the y-axis of the upper panel, with computed projections of the refinement mask, colored by defocus change; the patch CTF defocus for each particle is represented with a green square (in this case all the same as we used one patch).

Friday scripting - made a little CS-tools script to visualize the results of per-particle defocus refinement (here with the vault particle).

The bottom panel is supposed to be a pseudo-tomo view looking in the plane of the ice layer; min_ice is the inferred ice thickness based on the mask extent

Best 3D reconstruction so far from this Tundra dataset. Right shape with some hints of secondary structure. Data collected at a higher magnification will hopefully improve this. @nusbf.bsky.social #cryoEM

Postdoctoral Position in Membrane Protein Biology & Ion Channel Physiology Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

We’re hiring! Our group at CSHL has openings for postdoctoral fellows in structural biology with a focus on ion channels and receptors in neurotransmission and cancer immunology. Join and pursue unique, cross-disciplinary science to boost your career. jobrxiv.org/job/cold-spr...

2D classes of a 58 kDa particle collected on a 100 kev Tundra electron microscope

2D classes of the 58 kDa sample collected on the 100 keV Tundra TEM with a Falcon C detector at @nusbf.bsky.social @newcastleuni.bsky.social. Still much work to do to optimise the sample & data collection but its cool to see what a 100 keV TEM is capable of. #cryoEM @recon4imd.bsky.social

Made a little prototype extension so these notes can be read/written navigated in ChimeraX too - the idea is to have a kind of "track changes" for building/analyzing/discussing structures that is intrinsically linked to the relevant sites

PARP16 is a Druggable Regulator of Ribosome MARylation and Protein Homeostasis in Ovarian Cancer Cells www.biorxiv.org/content/10.64898/2026.04...

AlphaFold as a prior: experimental structure determination conditioned on a pretrained neural network Nature Methods - ROCKET improves experimental structure elucidation by integrating implicit structural knowledge from OpenFold, a trainable reimplementation of AlphaFold2, with X-ray...

ROCKET 🚀 inference-time optimization of AlphaFold to fit structural data is published! rdcu.be/fa9YH
Since our preprint, we’ve pushed it to regimes where other methods break: low resolution, weak signal, real experimental edge cases. Here’s what we learned: 1/15

Host cell remodeling via cyclin dependent kinases drives Ebola virus replication and transcription.

www.biorxiv.org/content/10.6...

Check out our latest preprint.

Great work by @tshamorkina.bsky.social. Thanks to co-authors Danni Snikkers and @hecklab.bsky.social

Cryo-electron microscopy scientist Join our team to contribute to the operation and innovation of state-of-the-art cryo-electron microscopes and empower groundbreaking research.

The newly established NeCEN Utrecht is looking for a #cryoEM scientist!

www.uu.nl/en/organisat...

Work with a new Krios 5 + pFIB and support cutting-edge life science research.

📍 Utrecht | 🗓 Apply by 30 April

Please share with your network!

During #RHIC's latest STAR collaboration meeting, members gathered by the STAR detector one final time before it is disassembled and the collider is transformed into the #ElectronIonCollider.

RHIC's operations have ended, but its science lives on — with years of data analysis and discoveries ahead.

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank pubmed.ncbi.nlm.nih.gov/41868252/ #cryoEM

Excited to share our work on the structure and function of cytoplasmic lattices within mouse embryos. A collaborative effort with @niakanlab.bsky.social and work led by @kashishsingh.bsky.social and @inaharasimov.bsky.social . It is now out on BioRxiv: www.biorxiv.org/content/10.6...

Added some of these features (notably copy-paste ligand) to the old coot-trimmings.

Also added a menu item to set the proportional editing radius, which changes the "sphere of influence" of refinement to allow easy moving of larger parts of the structure (e.g. domains).

github.com/olibclarke/c...

Finally got around to porting my coot-trimmings scripts over to Coot 1... only the hotkeys are working (nothing requiring creating a custom menu or GUI dialog), but I've added a couple of new ones, e.g. copy/pasting ligands.

Check it out & let me know what you think! 😊

github.com/olibclarke/c...

Paclitaxel compromises nuclear integrity in interphase through SUN2-mediated cytoskeletal coupling Summary: Paclitaxel induces nuclear aberrations in interphase through SUN2-dependent lamin A/C disruption, revealing mechanisms of action beyond mitotic arrest.

‼️Paclitaxel, the best-selling chemotherapy drug in history, may not act exactly as previously thought. It can profoundly alter nuclear morphogenesis during interphase, thereby disrupting critical cellular processes through perturbation of the nuclear domain. doi.org/10.1242/jcs....

We are recruiting! If you are passionate about technology development, protein engineering, computational design, directed evolution, chemical biology - please reach out!
(The setting is pretty nice too…)

My new spiritual eukaryote is ciliate in Si helmet swimming towards North pole ⚔️. Definitely an amazing reading from Bolzoni et al. "Magnetoreception in a freshwater ciliate arises from endosymbiosis." 10.1038/s41467-026-70462-8 for #protistsonsky

BRD4-mediated ER membrane contact creates functionally distinct mitochondrial subtypes Chen et al. used a reporter of endoplasmic reticulum-mitochondria contact sites (ERMCSs) to identify clinically relevant inducers of ERMCSs. They identified that fedratinib induces ERMCSs via inhibiti...

Distinct subtype of mito !! >> ETC complex III function is necessary for the formation of endoplasmic reticulum and mitochondria contact sites (ERMCSs). Congrats to whole team of Costas and Yatrik !!
www.cell.com/molecular-ce...

bedder-manuscript.v10 Bioinformatics, 2026, 0–0 doi: 10.1093/bioinformatics/xxxxx Advance Access Publication Date: DD Month YYYY Genome Analysis Genome Analysis bedder: flexible genome interval intersection with user-defi...

Brent Pedersen, Mitchell Vollger and I have "posted" our preprint of the manuscript describing bedder, a complement to the functionality of bedtools. The "preprint server" we have chosen is google docs because it was rejected by biorxiv.

docs.google.com/document/d/1...

New postdoc position at Cornell! This is a bit of a unique one: It's a teaching postdoc focused on integrating active learning strategies into psychology courses (including one of my own). Should be a lot of fun!

Apply here: academicjobsonline.org/ajo/jobs/31738

High-frequency biparental inheritance of plant mitochondria upon chilling stress and loss of a genome-degrading nuclease - Nature Plants Maternal inheritance of mitochondria breaks down in the cold when a mitochondrial DNA degrading nuclease is defective, resulting in biparental inheritance that can rescue mitochondrial mutations and g...

Mitochondria can indeed be inherited from the father! 🍀
In this new study, we uncovered the molecular mechanisms underlying mitochondrial inheritance in Nicotiana tabacum. @natplants.nature.com

doi.org/10.1038/s414...

We talk a lot about mitochondrial stress, but how about how do they keep themselves balanced? Good thing our speaker
@nieminm.bsky.social knows everything about that, and will tell all on the next MITOtalk. Usual time and place. See you there?