Mesmerizing light sheet imaging of neutrophils swarming at a yeast target!
The neutrophils were labeled with SPY650-DNA (yellow) and a calcium dye (blue).

This movie is part of the recent manuscript by @strickland-evelyn.bsky.social that we highly recommend reading!

www.biorxiv.org/content/10.6...

CRISPR–Cas-based live cell imaging of genome dynamics - Nature Reviews Genetics In this Review, Zhu et al. outline the various CRISPR-based tools recently built for dynamic DNA and RNA imaging, which have provided insights into various molecular mechanisms. The authors also discu...

(1/6) Watch the genome "dance" in real time. Our review in @natrevgenet.nature.com explores the CRISPR tools making it happen. Grateful for the guidance by Prof. Lei Stanley Qi @stanleyqilab.bsky.social and Prof. W.E. Moerner @moernerlab.bsky.social! #CRISPR #GenomeDynamics #Stanford #Imaging

Our latest publication by @jessel-ap.bsky.social is out in @cp-cellreports.bsky.social. We show that DNA damage in late mitosis triggers an ATM-dependent increase in RNF126 and BRAP, which promote cell survival under these conditions.
With help from @rfreirelab.bsky.social
doi.org/10.1016/j.ce...

My choice this week in @science.org
RCC1 depletion drives protein transport defects and rupture in micronuclei
doi.org/10.1083/jcb....

MRE11 proximal polyadenylation site-mediated looping impacts transcription and genomic stability Alternative polyadenylation (APA) generates transcript isoforms with variable 3′ untranslated regions (UTR) lengths, yet its role in DNA damage respon…

Out today in @cp-molcell.bsky.social!

We show that proximal polyadenylation site (pPAS) within MRE11 sustains its transcription through promoter–PAS chromatin looping.

Congratulations to first author Kaimeng Huang, all co-authors and collaborators.

www.sciencedirect.com/science/arti...

Tumour promotion through the lens of evolution Nature - This Review revisits tumour initiation and promotion in light of clonal diversity and the presence of cancer driver mutations in normal tissues, aiming to understand mechanisms that enable...

Tumor promotion through the lens of evolution

This review grew out of many long conversations, shared ideas, and lively discussions

It has been a real pleasure to think together with Allan, Paul, Eve, and Abel about how tumours develop and how carcinogens shape cancer risk

rdcu.be/fdC2J

Impact of Chromosomal Instability and Aneuploidy in Cancer Development Somatic human cells contain a diploid genome consisting of 23 pairs of chromosomes. The maintenance of this diploid state is essential across all layers of biological organization, ranging from the ph...

Pleased to share our review on chromosomal instability (CIN) and aneuploidy in cancer, led by Amanda Mennie. Now out @annualreviews.bsky.social!

www.annualreviews.org/content/jour...

Composite cryo-E M density map of the yeast K M N junction complex, showing the structure and interactions of K M N junction complex.

Happy to see our paper describing cryo-EM structures of the budding yeast KMN kinetochore complex published in @jcb.org! Our structure reveals how an important hub mediates and regulates assembly of the machine that segregates chromosomes. #cryo-EM @mrclmb.ac.uk

🔗 rupress.org/jcb/article/...

Excited to share our structural insights into how microtubules differentially guide phosphorylation of kinetochore-microtubule regulators, Ndc80 and MCAK, for chromosome segregation. Heroic efforts by Yiming Niu with a fun collaboration with Jennifer DeLuca lab!
www.science.org/doi/10.1126/...

Our H1 paper is out #ScienceAdvances:
www.science.org/doi/10.1126/...
@masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in live cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

Very exciting that our new paper on the forces exerted by the Rad51-Rad54 homology search complex is now online. This was a collaborative effort with the Wang here at Cornell, and this work performed primarily by a graduate student, Mitch Woodhouse
genesdev.cshlp.org/content/earl...

Our new review on NHEJ just came out on G&D genesdev.cshlp.org/cgi/content/... Highlight the 5 phases, and importantly, the 5 protein bridges that bring the 2 DNA ends together for NHEJ. Thank Dr. Geunil Yi, for generating the illustration for all structural models.

Why do mammalian cells need PAR, PARP1/2, but yeast does NOT? While studying the impact of PARG inhibition, Isaac in the lab found that extra-PAR sequesters nuclear PAR-binding proteins (XRCC1 and its partners, etc.) into nuclear condensates. biorxiv.org/content/10.6...

Dear all, please see link below for Milano et al, describing a role for BRCA2 (& BRCA1) in the maturation of nascent DNA strand gaps during DNA replication in the presence of PARP inhibitor. Congratulations Larissa and all !

authors.elsevier.com/a/1mtQe3vVUP...

Tetraploids or polyploid giants: who is truly dangerous? Tetraploidy, resulting from a single whole-genome duplication (WGD) event, contributes to tumorigenesis by promoting genomic instability and functional diversity. In general, WGD beyond tetraploidy limits proliferative and tumorigenic potential, but an increasing number of studies suggest that polyploid giant cancer cells (PGCCs)—large, highly polyploid (≥8N) cells formed in response to chemotherapy—produce daughter cells with reduced DNA content that drive cancer progression. In this opinion article, we examine the literature on tetraploid cells and PGCCs from a cell biology perspective. It is our opinion that the role of tetraploidy in cancer is supported by findings from cell lines, animal models, and tumor sequencing data, while definitive evidence that viable progeny from PGCCs can promote cancer progression in human tumors is lacking.

Tetraploids or polyploid giants: who is truly dangerous?

Proud to share the yeast telomerase structure, led by the talented @hongmiaohu.bsky.social in collaboration with the Wellinger and Chartrand labs. Discovered 37 years ago and took us nearly 7 years but totally worth the wait 😍.
www.science.org/doi/10.1126/...
www.youtube.com/watch?v=gFE4...

Our lab at the UC Irvine is currently hiring a postdoctoral researcher. We have a thriving and growing biomedical research campus and are looking for candidates with a strong interest in DNA damage and repair, cancer etiology, and cancer health disparities. Apply at recruit.ap.uci.edu/JPF09440

Spindle pole proteins confine chromosomes to ensure their expulsion during female meiosis www.biorxiv.org/content/10.64898/2026.03...

Delighted to see our work now published at the EMBO Journal! Check also this concomitant paper by the Bai and Mirny labs with an orthogonal approach that aligns well with our measurements www.nature.com/articles/s41... Great system to study how SMCs facilitate/regulate target search in chromatin!

T-loop dynamics: telomere structure shapes cell fate decisions Telomeres are nucleoprotein elements bound by shelterin that protect chromosome ends from DNA damage signalling and inappropriate repair. A defining architectural feature is the telomere loop (t-loop)...

“T-loop Dynamics: Telomere Structure Shapes Cell Fate Decisions” is out now in @cp-trendscellbio.bsky.social .

www.cell.com/trends/cell-...

It was a pleasure to work on this with collaborator and friend @makotothayashi.bsky.social, whose insight was central to shaping the ideas in this review.

I’m thrilled to share our new review in @cp-trendscellbio.bsky.social! This was a wonderful collaboration with my friend ‪@thecesarelab.bsky.social‬, and I truly enjoyed our writing process. We explore "T-loop Dynamics: Telomere Structure Shapes Cell Fate Decisions." x.gd/h0UGl 🧵

We are hiring POSTDOC now!🚨please repost! Fully-funded 5-year position 👩🏻‍🔬👨‍🔬🧬Looking forward to your application!✉️

Replicative gaps in DNA damage tolerance, genome instability, and cancer therapy Replicative single-stranded DNA gaps arise when DNA synthesis proceeds past lesions or difficult-to-replicate regions. This review delineates the pathways that generate, stabilize, and process these i...

Excited to share our new review in Molecular Cell on replicative single-stranded DNA gaps and their role in genome instability and cancer therapy.

#DNARepair #ReplicationStress #GenomeStability #CancerResearch #MolecularBiology #PARPinhibitors​​​​​​​​​​​​​​​​

www.cell.com/molecular-ce...

Microtubule end stabilisation by cooperative oligomers of Ska and Ndc80 complexes - The EMBO Journal During mitosis, properly aligned chromosomes stabilise microtubule ends with the help of kinetochores to ensure timely segregation of chromosomes. Microtubule-binding components of the human outer kin...

Happy to see the first paper from the lab published in the EMBO J today: link.springer.com/article/10.1...

In it, we reconstitute self-assembling cooperative oligomers of human Ndc80 and Ska kinetochore complexes that stabilise microtubule ends, and study these samples using cryoET and TIRF

Somatic genomics as a discovery engine for biomedicine Somatic genetics uses tissue mutations to understand aging, reveal disease mechanisms, and guide new therapies.

ONLINE now in @cellpress.bsky.social: A review by CRI's @haozhulab.bsky.social about their #relentlessdiscovery and how somatic mutations are becoming a key discovery engine for disease genetics. 🧪 Read more ⬇️
www.cell.com/cell/fulltex...

Happy to share our new work on the nuclease EXO1, published in Nature Communications. We show that EXO1 is overexpressed in cancers. This promotes nascent DNA degradation, even in BRCA-proficient cells, resulting in genomic instability and chemotherapy sensitization. www.nature.com/articles/s41...

I had fun writing this N&Vs on two fascinating new papers on centromere evolution in yeast. If you’re interested in how centromeres adapt yet stay functional, here’s my take. rdcu.be/e6DD0 @nature.com @gautamdey.bsky.social @gsherloc.bsky.social @helsenjana.bsky.social @maxhaase.bsky.social

The Sfeir Lab at MSKCC is hiring a Research Assistant!
We are looking for a motivated scientist with at least 2 years of research experience and a two-year commitment. Learn more about our work here www.sfeirlab.com, and message me your CV and cover letter if interested

Paper alert from @codyz.bsky.social y.social and Yang Liu. New mechanism for chromatin remodeling by ATRX. Super cool story. Congrats to #lugerlab alumni.
www.biorxiv.org/content/10.6...

Concurrent L1 retrotransposition events promote reciprocal translocations in human tumorigenesis LINE-1 (L1) retrotransposition generates somatic genomic variation in human cancer, but short-read sequencing has limited our understanding of its structural consequences and dynamics. Using long-read...

Today in
@science.org:
We are pleased to present our last work entitled:
"Concurrent L1 retrotransposition events promote reciprocal translocations in human tumorigenesis"
by Zumalave et al.
www.science.org/doi/10.1126/...