📣 Physicists and engineers: apply your experimental skills to biology! Join the group of Katerina Naydenova @imprs-lm.bsky.social for a #PhD to develop new technologies that enable answering fundamental biological questions. Apply NOW!

GitHub - Lucaslab-Berkeley/Leopard-EM: Two-Dimensional Template Matching implemented in Python Two-Dimensional Template Matching implemented in Python - Lucaslab-Berkeley/Leopard-EM

Since submission, we have a new version of the program with some improvements, and we have many developments in the pipeline for faster, more sensitive template matching. Keep an eye on our GitHub for these.
github.com/Lucaslab-Ber...

Leopard-EM: an extensible 2D template-matching package to accelerate in situ structural biology In situ cryo-EM coupled with 2D template matching (2DTM) holds the potential to visualize the cellular structureome in context, but further developments are required to make this a reality. We describ...

Now out in full form. Again, a big thanks to #teamtomo and @alisterburt.bsky.social for developing the infrastructure to build this on. journals.iucr.org/d/issues/202...

Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing

Read more: www2.mrc-lmb.cam.ac.uk/a-new-method...

#LMBResearch

We are happy to announce that we will have two new scientific groups led by Andrija Sente and Katerina Naydenova soon! If you are interested to join their lab as a PhD Student apply at our @imprs-lm.bsky.social

www.imprs-lm.mpg.de/index.php/jo...

Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching www.biorxiv.org/content/10.1101/2025.09.11.675606v1 #cryoEM

Happy for this to be out! We have documentation and tutorials here. Please give it a try and let us know what you think. lucaslab-berkeley.github.io/Leopard-EM/

Pulsed-electron illumination does not reduce beam damage for imaging biological macromolecules www.biorxiv.org/content/10.1101/2025.07.29.667395v1 #cryoEM

Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS The physical limit in determining the atomic structure of biological molecules is radiation damage. In electron cryomicroscopy, there have been num...

@joshdcryoem.bsky.social will also be giving a talk about his prior work as a PhD student with Chris Russo developing strategies to take advantage of the reduced radiation damage at liquid Helium temperatures for cryo-EM. You can find their paper here: www.pnas.org/doi/abs/10.1...

To tilt or not to tilt? Strategies for in situ cryo-EM data collection Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol…

If you are going to M&M, also see postdoc @joshdcryoem.bsky.social's poster about tilted vs untilted data collection for in situ structural biology www.sciencedirect.com/science/arti....

To tilt or not to tilt? Strategies for in situ cryo-EM data collection Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol…

Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...

At the 2024 IMC meeting, Richard Henderson laid down a challenge. Design a #TEM single-particle #cryoEM lens with 1mm Cc at 100kV. I'm thrilled to reveal our new Arxiv preprint delivering a fully retrofittable pole-piece swap out for the @jeoleurope.bsky.social 1400 TEM.
arxiv.org/abs/2505.11458

In the Bay Area? Love computational cryo-EM/ET?

Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!

Details and registration at frostbyte-cryoem.github.io

Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!

For a shorter read, @mrclmb.bsky.social and @science.org also have articles about the helium story. www.science.org/content/arti...

Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS The physical limit in determining the atomic structure of biological molecules is radiation damage. In electron cryomicroscopy, there have been num...

After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...

Exploring shaped focused ion beams for lamella preparation www.biorxiv.org/content/10.1101/2025.04....

In-Situ High-Resolution Cryo-EM Reconstructions from CEMOVIS www.biorxiv.org/content/10.1101/2025.03....

Foam film vitrification for cryo-EM www.biorxiv.org/content/10.1101/2025.03....

I've been wanting to see a paper using off-axis electron holography for low-dose biological imaging since the start of my PhD. Spatially resolved charge measurements👌

A new statistical metric for robust target detection in cryo-EM using 2D template matching A novel statistical metric for 2D template matching (2DTM), the 2DTM p-value, has been developed to improve the detection of targets in cryo-EM images under various imaging and sample conditions, particularly for smaller and aspherical targets.

Kexin Zhang et al.: A new statistical metric for robust target detection in cryo-EM using 2D template matching #3DReconstructionAndImageProcessing #SingleParticleCryoEM #Imaging @umassboston...#IUCr https://journals.iucr.org/paper?S2052252524011771

Laser Flash Melting Cryo-EM Samples to Overcome Preferred Orientation biorxiv.org/cgi/content/short/2024.11.21.624652v1

It should be relatively simple with the Bio.PDB package in Biopython. It has both pdb and mmtf IO modules.