2D classes of a 58 kDa particle collected on a 100 kev Tundra electron microscope
2D classes of the 58 kDa sample collected on the 100 keV Tundra TEM with a Falcon C detector at @nusbf.bsky.social @newcastleuni.bsky.social. Still much work to do to optimise the sample & data collection but its cool to see what a 100 keV TEM is capable of. #cryoEM @recon4imd.bsky.social
Blender Rendering of vitrified HA trimer with DM at the air-water interface
We identified the maltoside detergent DM as an effective and easy solution to address preferred orientation in cryo-EM. Helped me switch from graphene grids. Special congrats to Maria Rafiq! @landerlab.bsky.social @nanigrotjahn.bsky.social @wisemanlab.bsky.social #cryoEM doi.org/10.64898/202...
For a plain-language overview of our work, see this lay summary courtesy of Kudos:
www.growkudos.com/publications...
Our paper describing the #cryoEM structures of unusual spike proteins from marine mammal–infecting gammacoronaviruses is now published in PLOS Pathogens! 🐬
journals.plos.org/plospathogen...
Depictions of the structure of CDK11-cyclin L-SAP30BP. Four cryo-EM densities are shown at the top, two molecules models at the bottom. CDK11 is green, cyclin L is purple, and SAP30BP is yellow.
Have a look at our latest results on #CDK regulation, just posted as a pre-print! This study was spearheaded by PhD student Amy McGeoch. Amy has determined the #cryoEM structure of the CDK11-cyclin L-SAP30B complex, an important regulator of #spliceosome activation.
www.biorxiv.org/content/10.6...
@embo.org Practical Course: Advances in cryo-electron microscopy and 3D image processing
📅 17-25 August 2026
📍 @embl.org Hamburg
📝 Registration Open| Deadline: 11 May 2026
🔗 instruct-eric.org/events/embo-...
#cryoEM #Training
The newly established NeCEN Utrecht is looking for a #cryoEM scientist!
www.uu.nl/en/organisat...
Work with a new Krios 5 + pFIB and support cutting-edge life science research.
📍 Utrecht | 🗓 Apply by 30 April
Please share with your network!
The E3 ubiquitin ligase mechanism specifying target-directed microRNA degradation (TDMD) is now published! 🎉🍾 We, @bartellab.bsky.social and Schulman lab, describe how 2-RNA factors control protein degradation by recruiting an E3 ligase. @mpibiochem.bsky.social
www.nature.com/articles/s41...
Cryo-EM success starts before the microscope.
Join us @royalmicrosoc.bsky.social for a free Cryo-EM SPA mini-course (1 of 3) on sample prep.
Learn purification, grid prep & screening tips + live Q&A.
🗓️ Apr 14
Register once to attend all 3 - Sample Prep, Collection, Processing
👉 ter.li/pksdqb
Excited to welcome @tejashyamk.bsky.social to the ERC starting grant team, as he starts his PhD!
Teja joins us from @sc-lab.bsky.social, where he built a solid foundation in biochemistry & #cryoEM 🔬
He’ll be uncovering new insights into norovirus replication and assembly. Exciting science ahead!
February Map of the Month: Aca2–RNA
We reprocessed EMPIAR-11918 and achieved 2.76 Å in 50.5 compute hours.
Sub-3 Å at ~40 kDa is a strong example of what modern cryo-EM workflows (and RELION with Blush) can deliver for small targets.
📧 hi@cryocloud.io
#CryoEM #CryoCloud
Structural insights into Cas9-mediated prespacer selection in CRISPR-Cas adaptation pubmed.ncbi.nlm.nih.gov/41702403/ #cryoEM
Not every day your work helps protect baby elephants 🐘💙
Super proud to have contributed to the cryo-EM part of this project - it also happens to be my 20th publication, which makes it feel extra special ☺️
Honoured to be selected as one of this year’s EMBO Young Investigators!
Grateful to my team and colleagues for all their support, and excited about the opportunities this will create for my group and for our work in structural virology.
www.embo.org/press-releas...
WeINSPIREBio’s mission is to empower, connect, and elevate women scientists working in structural biology and structure-based drug design.
If you’d like to follow updates from the community, here’s the link you the WeINSPIREBio group on LinkedIn: www.linkedin.com/groups/13099...
🌟 WeINSPIREBio is hosting its first event!
On Dec 4, we’re running a webinar:
Structures of Success: Career Journeys of Women in Structural Biology
If you work in SB, drug discovery, or just like hearing career stories from brilliant people, join us! And please share 🙏🏼
lnkd.in/ewjD5WPt
#cryoEM
Heading to #PSDI2025 in Basel! Excited for good science, great discussions and familiar faces. Come say hi or book a chat with us!
Thrilled, and admittedly relieved, to see this major investment in Dutch #cryoEM become reality! 🇳🇱🔬
Honoured to be a co-applicant on this national initiative and very excited for the opportunity to advance our work in cellular structural virology! 🦠
www.uu.nl/en/news/33-m...
😬
Yikes! In this paper the AlphaFold model of human plasminogen (PDB-8uq6) has been refined into noise (EMD-42462) using Phenix & ISOLDE. For some reason the authors even interpret side-chain interactions:
#cryoEM
✨Excited that the main project of my PhD is now available as a pre-print on #bioRxiv
Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo
🔗 www.biorxiv.org/content/10.1...
Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup).
A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
📣 Live from the Dutch Structural Biology Meeting in Delft! Robert (@riborob.bsky.social) is presenting our CryoCloud poster on accelerating #cryoEM workflows. We’re also sponsoring the Poster Prize 🧦🎉 Big thanks to Arjen Jakobi (@cryotud.bsky.social) for organising. Excited for today’s discussions!
September Map of the Month: Small Data, Big Insights ⚡️
From just 233 movies, CryoCloud delivered a 3.2 Å SARS-CoV-2 spike map in 3.5 h.
Fast and efficient #cryoEM, exactly what vaccine design teams need when screening dozens of antigen variants per day.
📬 DM or hi@cryocloud.io for more info!
Coming up next week: the third Dutch Structural Biology meeting! Now in Delft, on Sept 26. See program here: nvbmb.kncv.nl/biology-2025 with #teamtomo #cryoEM #nmrchat simulations and more (looks like)
Organized by the #NVBMB (associated with KNCV)
So happy to have you on board, @jbrwhite.bsky.social!
A few snaps from @cryocloudio.bsky.social retreat in the Belgian Ardennes 📸🌿
We mixed focused work sessions with walks and shared meals, late-night Jenga battles, and plenty of time socialising. We even spotted deer in the garden 🦌
And yes, we also launched our own #cryoEM socks!
