We introduce mirror-enhanced 4Pi-SMLM (me4Pi-SMLM), a streamlined single-objective configuration that leverages mirror-based retroreflection of the illumination beam to achieve near-isotropic localization precision of 2–3 nm, enabling unambiguous resolution of diverse ultrastructural features.
🚨 PhD position 🚨
Fully funded PhD position at the intersection of super-resolution microscopy, glycobiology, and biomedicine!
The position is part of a Doctoral Network. All information: www.glycocalyx.org
Apply here: tinyurl.com/yc35dajz
Spread the word!
Now out in @natcomms.nature.com: www.nature.com/articles/s41...! The latest GitHub commits/Colab notebooks enable simulation with 3D sequences.
Just channeling Stephen J Gould: "I am, somehow, less interested in the weight and convolutions of Einstein's brain than in the near certainty that people of equal talent have lived and died in cotton fields and sweatshops"
We've now discovered some neat cell culture dishes that feature a 50 micron thick film of a proprietary polymer with refractive index even closer to water than FEP. They work just as well as our homemade FEP assembly and are available in multiwell plate format too!
www.sarstedt.com/en/products/...
We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues.
www.biorxiv.org/content/10.1...
🚨 2 × PhD positions @EPFL! 🚨
Help us push the boundaries of fluorescence microscopy - DNA nanotech, custom optics & spatial omics in Lausanne 🇨🇭. Start Jan 2026. Send CV + motivation + 2 refs → fschueder@ethz.ch
#PhD #Hiring #microscopy #SuperResolution #SpatialOmics #DNAPAINT #FLASHPAINT
Francesca Bottanelli
Our ‘Cell Scientist to Watch’ is Francesca Bottanelli @franbottanelli.bsky.social. We discover more about Fran’s career path, her approach to scientific mentorship and her dream to start a band!
journals.biologists.com/jcs/article/...
a cycling computer displays a text message from a friend “We’re breaking into your house”.
a cycling computer displays a text message from a friend “I have catnip”
I love the weekend
Two women sit in large wooden arm chairs worn leather cushions in a high ceiling room. To the right a projector screen shows multicolor images of cells.
The AQLM Queens of Super-res lead evening discussion. Dr. Gustavsson and Dr. Lidke help us figure out how to look REALLY close. #AQLM2025
@gustavssonlab.bsky.social
@aqlm.bsky.social
Well, maybe the children will have to live with two cures instead of 30 cures.
Everyone please read and act! 👇
Whew! Hakeem had time today, and you should read what he had to say. This piece is excellent!
Nice work @zacsimile.bsky.social - interesting results!
I was invited, for July, to a scienctific meeting in Telluride (Colorado). I was told it is a wonderful place for a conference, and I was very much looking forward to an exciting meeting in a fantastic environment. I have just canceled my participation, despite having booked already everything.
Your weekly reminder that it is not ok to out or misgender trans or nonbinary individuals whether it is in a public forum, workplace, or anywhere else. It’s harassment, and a real distraction from everything else.
By putting a donut between our faces and each article we read, we were able to extract more information than ever before about MINFLUX. You can read the new review article from @maxperutzlabs.bsky.social by Lukas Scheiderer, myself, and @jonasries.bsky.social at www.nature.com/articles/s41...
Folks stand around a plaza after the Stand up for Science rally in Palo Alto on a beautifully sunny day
It’s a beautiful day to #StandUpForScience
We all owe @jeremymberg.bsky.social a debt of gratitude for his work in clarifying, connecting, and advocating for science in this chaotic time.
🙏🏼
nothing wakes you up like getting your representative’s office on the phone over your morning coffee
Our new OPM system, in which the camera is placed directly after the secondary objective to directly detect the oblique plane.
In our work, we just put the camera directly into the remote refocus volume to detect the oblique plane directly, without the need for a tertiary microscope system!
The self-driving multiscale microscope from @daetwylerstephan.bsky.social is out.
It can image an entire zebrafish embryo longitudinally and follow a volume of interest over time with high resolution.
We used it to study cancer-immune cell interactions.
www.nature.com/articles/s41...
Ardem, I am sorry. In this middle of this chaos, HHMI will always hold the dubious distinction of being the first organization, federal or private, to cut an ongoing and funded DEI program. In the darkest hour, it ran for the hills. As a member of those groups, I can’t tell you how painful this is.
I urge everyone to read this concise but very complete history of what's been happening at our nation's science agencies the past few years, I mean days, by @dereklowe.bsky.social www.science.org/content/blog... TY Derek
Incredible new work from Yongdeng Zhang demonstrating live 4Pi SIM--and in two colors! www.nature.com/articles/s41...
Reading the paper in Cell about how to close the scissor plots in gender representation at senior academic positions, I feel just a touch of pessimism. Having reached a level of seniority (middle management) I didn’t think I ever would, I read these great ideas wanting more, much more /1.
Oh wild - thanks for sharing, David! Hadn’t thought to check that. Unfortunately it is either the table, or I have precious few (non-)bridging components which are reasonably toleranced. Can see posts tilt when tightening them in some places.
Anyone have experience with this #MicroscopistNightmares? This table is warped in several (many?) places. Is it silly to worry about further warping and alignment instability?
Hey #microscopy Bsky… what is that xz wormy PSF 😭😭😭😭
Thanks, Izzy!
