Extrinsic factors can create a local tissue environment that encourages restoration @science.org
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
@canaztekin.bsky.social
www.science.org/doi/10.1126/...
@storerlab.bsky.social @scicambridge.bsky.social @kevinchalut.bsky.social
It was really fun to work on this collaboration with @storerlab.bsky.social and Byron - I think we are really starting to see that ECM is more than just a scaffolding. It's really driving tissue function in important ways, like in this application of tissue repair.
🚨 New Preprint from the Paluch & Chalut Labs 🚨
Ana Raffaelli et al show that the stiffness of substrate (in vitro) / basal membrane (in vivo) determine how cells interpret biochemical signals (BMP4) and which fates they acquire
#mechanobiology #hESCs #substratemechanics
doi.org/10.64898/202...
I think this work demonstrates some broad principles about the coupling between ECM, epithelial polarity, developmental signalling and mechanotransduction. This work represents a really fun collaboration with Ewa’s lab, and is a testament to the determination, rigour and brilliance of Ana.
Finally, what I think is possibly the most interesting result in the paper, we could phenocopy the soft basement membrane phenotype completely (loss of patterning) using soluble laminin, which disrupts epithelial polarity independently of the basement membrane.
Second, softening of the basement membrane in the mouse embryo using a LOX inhibitor leads to ectopic, premature differentiation of mesendoderm lineage;
This is due to a disruption in the FAK-PI3K regulation of epithelial polarity, which leads to a loss of the apical-basal polarization of BMP receptors (in other words, the cells don’t know up from down and the BMP receptors go everywhere);
A few highlights from this work: first, in a human pluripotent stem cell model of gastrulation, we showed that by simply softening the basement membrane, the spatially patterned response to BMP signalling is lost.
Excited to share this new work from Ana Raffaelli, in collaboration with Ewa Paluch's lab and involving several of my colleagues at Cyclana Bio. In this paper, we show that basement membrane mechanics instructs developmental signalling and fate patterning.
www.biorxiv.org/content/10.6...
Excited to share our work on epithelial multilayering - identifying why stem cell stay in the basal layer and how and why differentiating cells move up. Great collab with @manningresearch.bsky.social and Niessen labs! Check out preprint and great summary below www.biorxiv.org/content/10.6...
Congratulations Agathe and team!
Very excited that the lab's first paper is out! We showed that in stem cells, Aurora B controls abscission dynamics by regulating microtubules stability and MCAK recruitment. Very happy with this streamlined version of our 2024 preprint.
www.cell.com/cell-reports...
We are really proud to share the first 'Pre-Print' from the Storer lab driven by the fantastic Byron Mui, and co-lead by the innovative Kevin Chalut, on tissue mechanics, ECM and regeneration. A big thanks also to our collaborators in the Franze Lab.
www.biorxiv.org/content/10.1...