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🫤 is the peak splitting effect reproducible with a different column?

Is the sample dissolved with higher organic content than your weak mobile phase?

Protein Contaminants Matter: Building Universal Protein Contaminant Libraries for DDA and DIA Proteomics Mass spectrometry-based proteomics is constantly challenged by the presence of contaminant background signals. In particular, protein contaminants from reagents and sample handling are almost impossi...

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pubs.acs.org/doi/10.1021/...

With acknowledging you mean making them co-authors, right?

Impact of Local Air Pressure on Ion Mobilities and Data Consistency in diaPASEF-Based High Throughput Proteomics pubs.acs.org/doi/10....

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#proteomics #prot-paper

I spend a bit of time talking to folk with plasma cohorts about MS vs affinity approaches for discovery. Thought it was worth a thread.

Most love the marketing. 5,000 proteins? 10,000 proteins? OLink was used in UK Biobank? Wow, mass spec is sooooo last year. Except life is not that simple.

I like the matix-matched calibration curve benchmarks from one of the first papers with the Orbitrap Astral: pubs.acs.org/doi/10.1021/...

It also becomes tricky when studying protein synthesis regulation. Many treatments or stresses in cells cause inhibition of translation and standard normalisation approaches do not work well in such cases 😵‍💫