Make your own PDMS+micromirror device for single-objective light-sheet SMLM: www.biorxiv.org/content/10.6...

"Why is publishing so expensive?" It's a question I hear all the time, so we at @biologists.bsky.social thought we'd try to dispel some of the myths around publishing economics & provide transparency around our costs. Today, we publish editorials in all 5 of our journals - links in the thread below.

The Idea: a searchable online database for core characteristics of molecular motor (in vivo & in vitro data) + link to the actual publication.

The Problem: while I know a bit about molecular motors, I know much less about setting up such databases. Anyone with such experience interested in joining?

Registration deadline for our motors and filaments meeting is tomorrow - not a 1st April joke! Thanks to our generous sponsors supporting sustainable scientific conference organisation @embo.org @biologists.bsky.social we also offer registration for a free livestream, supported by bsky discussions!

Chief Executive Officer | Inspired Selection

A unique opportunity to lead the most wonderful company in scientific publishing (I might be slightly biased here). The Company of Biologists are searching for a CEO.

www.inspiredselection.com/jobs/jo00000...

#Neurons rely on molecular motors to deliver proteins to precise locations. Jiang, Ichinose, Hirokawa et al. show that #kinesin-2 motors form heterogeneous assemblies with distinct cargo preferences: A KIF3B-enriched assembly preferentially associates with TRIM46 rupress.org/jcb/article/...

Join us for the next DGZ Focus Workshop, next week Tuesday, organized by Franziska Lautenschläger & Anne Straube, featuring topics around #cytoskeleton and #molecularmotors.
Talks by @lilianpaty.bsky.social , Rhoda Hawkins, Roman Podhajecky & Kanako Iwasaki

Proud to share the yeast telomerase structure, led by the talented @hongmiaohu.bsky.social in collaboration with the Wellinger and Chartrand labs. Discovered 37 years ago and took us nearly 7 years but totally worth the wait 😍.
www.science.org/doi/10.1126/...
www.youtube.com/watch?v=gFE4...

Logo Weiß auf Blau mit Schriftzug: open research europe

Deutschland beteiligt sich an "Open Research Europe" #ORE. Über die Plattform können Wissenschaftler*innen aller deutschen Forschungseinrichtungen ihre Ergebnisse künftig kostenfrei veröffentlichen u. im offenen Verfahren begutachten lassen. Die DFG ist mit der Durchführung beauftragt:
sohub.io/rqll

#ServiceNotification SiR-DNA completely blocks Anaphase of hTERT-RPE1 cells during time lapse recordings (every 3min) for mitotic progression analyses at concentrations >/= 5nM (@1h preincubation). No DNA staining with 1nM. Visible staining AND progression beyond Anaphase with 3nM SiR-DNA only.

"Dear Company, I work at a university (with ~10k employees and an annual budget of 800 Mio € that has been around for more than 500 years) and would like to order something from you"

"Sorry we only sell to businesses - show us proof of your business licence"

#GermanyInANutshell 🤷🏼‍♂️

Microtubule end stabilisation by cooperative oligomers of Ska and Ndc80 complexes - The EMBO Journal During mitosis, properly aligned chromosomes stabilise microtubule ends with the help of kinetochores to ensure timely segregation of chromosomes. Microtubule-binding components of the human outer kin...

Happy to see the first paper from the lab published in the EMBO J today: link.springer.com/article/10.1...

In it, we reconstitute self-assembling cooperative oligomers of human Ndc80 and Ska kinetochore complexes that stabilise microtubule ends, and study these samples using cryoET and TIRF

Hi all fans of #smart #microscopy,

we from @zeiss-microscopy.bsky.social have just published a new application note: "Study Mitotic Progression with Guided Acquisition – Rare Event Detection"

go2.zeiss.com/ODk2LVhNUy03...

For more details: forum.image.sc/t/applicatio...

Cordula Reuther, Paula Santos-Otte, Stefan Diez and colleagues find that microtubule lattice defects facilitate spastin-mediated severing.
journals.biologists.com/jcs/article/...
#OpenAccess #ReadandPublish

The handle you are looking for is @stefandiezlab.bsky.social 😉

So what are your experiences using fluorescent life cell DNA stains to follow mitotic events? Is it just picking the lesser evil between burning the cells (Hoechst33342), sticking all chromosomes together (SiR-DNA) or messing the cells up with a virus (CellLight) or is there really a GOOD solution?

Optimizing spatial organization of FtsZ rings for large-scale constriction in synthetic cells - Nature Communications Spatially regulated membrane constriction is an important milestone in reconstituting minimal cell division. Here the authors engineer a truncated system where the cytosolic domain of FtsN forms large...

Neat paper from Schwille lab. They demonstrate constriction of synthetic vesicles using important components of the bacterial cell division machinery, FtsZ and FtsN. They induce strong constriction of Z-rings in vesicles just by adding the cytoplasmic domain of FtsN www.nature.com/articles/s41...

Grafik mit goldenem, geschwungenem Weg und Bäumen, Text: „Excellent – today and in the future. University of Excellence 2027–2033“.

Grafik mit Text vor grünem Hintergrund: Fünf Ziele in der neuen Förderphase Die in der vergangenen Förderphase begonnenen Projekte werden fortgeführt beziehungsweise weiterentwickelt. Dabei wird die Universität Tübingen mit den Mitteln aus der künftigen Exzellenzförderung fünf Ziele verfolgen: Exzellenz in Forschung und Lehre Aufbau eines integrativen und kooperativen Universitätsumfelds Flexibilität und Anpassungsfähigkeit in einer sich schnell verändernden Welt Globales Bewusstsein und internationale Vernetzung Gesellschaftliches Engagement, Innovation und Ko-Kreation

Logowand: Exzellenzstrategie, Wissenschaftsrat, DFG sowie Förderhinweis mit Logos von Bundesministerium für Forschung, Technologie und Raumfahrt und Baden-Württembergischem Wissenschaftsministerium.

Die Universität Tübingen bleibt Exzellenzuniversität! 🎉 Dies hat eine Kommission aus Wissenschaftlerinnen und Wissenschaftlern sowie Vertreterinnen und Vertretern von Bund und Ländern am Abend bekannt gegeben: 👉 uni-tuebingen.de/universitaet/… #ExStra #Forschung #UniTübingen #Exzellenzstrategie

Well then, now you have got the opportunity to tag other people, making them aware of the issue and inviting them to join the conversation 🙂 maybe we could use #KinesinRevision_2.0 ?

... and then of course all together also stick to that agreement 😉

... 2+ different classifications of kinesin subfamilies side by side: could we please all sit together and agree on one nomenclature and one classification (which of course might be subjected to future changes) to go on from here?! Please (with a huuuge cherry on top)!!! It's a mess! 2/2

Given that the last proper position paper on the classification and nomenclature of kinesins is more than 20 years old and our knowledge of kinesins has vastly expanded during this time, but people in the field are still using 3+ different names for the same kinesin and ... 1/2

Commercial CO2-cylinder of a water carbonator connected to a scientific technical device that produces a mixed atmosphere for cells during live cell microscopy.

What's good for us can't be bad for our cell lines... eh?!🤷🏼‍♂️

To enable reuse by the research community we're making the two parental FPBase libraries available on @addgene.bsky.social as pooled plasmid libraries (Addgene #245482 #245483). Use them. Shuffle them. Train on them! addgene.org/Calin_Plesa/ 12/n

We are very excited to share a new resource from our team: spatial subcellular proteome maps in plants! We developed an MS-based method that registers localizations of about 8000 proteins in Arabidopsis roots in a single experiment.

(1/9)
www.biorxiv.org/cgi/content/...

ATTENTION #tubulin fans! We have another #openaccess #Techniques and Procedures paper in #CYTOSKELETON! Expression, Purification, and #Microscopy-Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 #Human Tubulin. Read it here: onlinelibrary.wiley.com/doi/10.1002/...

Microscopy images showing the heterogeneity of cultured astrocytes.

Chieko Ikoma, Kodai Inoue, Asako Terasaki and colleagues use their improved culture and transfection methods to visualise cytoskeleton structures in primary cultured astrocytes.
Highlight: journals.biologists.com/jcs/article/...
Article: journals.biologists.com/jcs/article/...

ATTENTION #KINESIN FANS!!! We have an #openaccess Techniques and Procedures paper for you in #CYTOSKELETON! Protocol for Reconstituting Adaptor-Mediated Activation of Full-Length Kinesin-1. Read it here: onlinelibrary.wiley.com/doi/10.1002/...

The peer reviewed and updated version of this work is now online (although not quite the fully formatted version). We had an existential crisis about kinesin-1 regulation while developing this work, but we are super proud of everything we put into this project. www.cell.com/iscience/ful...

image of the OpenFlexure Microscope on the left. On the right, our adapted live-cell imaging openflexure microscope with cradle to negate vibrations inside an existing incubator

Unleashing live-cell imaging for the masses!
Adapting the OpenFlexure Microscope for Affordable Live-Cell Imaging www.biorxiv.org/content/10.6...
Fits into existing incubators for time-lapse experiments & feeds into existing bioimaging analysis pipelines.
Thanks @wellcometrust.bsky.social funding