#ServiceNotification SiR-DNA completely blocks Anaphase of hTERT-RPE1 cells during time lapse recordings (every 3min) for mitotic progression analyses at concentrations >/= 5nM (@1h preincubation). No DNA staining with 1nM. Visible staining AND progression beyond Anaphase with 3nM SiR-DNA only.

Panel of immunofluorescence images showing DAPI (upper right, purple), anti-KIF15 (mid-left, orange) and anti-FLAG (mid-right, cyan) of metaphase RPE1 KIF15 KO cells that have been rescued with a eGFP-FLAG-KIF15-WT construct. Panel on the lower left shows a stained interphase cell for comparison.

#ServiceNotification for the few that work with #KIF15 : please note that the polyclonal KIF15 AB #55407-1-AP by #proteintech creates lots of specific-looking background in IF (especially in interphase cells) that IS NOT KIF15! As tested in a KIF15 KO cell line rescued with a FLAG-tagged WT KIF15.