This changes how we think about metabolism-epigenetics coupling.

Congrats to first author Toni Gañez 🍾
@crg.eu @erc.europa.eu @cienciagob.bsky.social

🧬 Phosphatidylinositol signaling (PIP2) dynamically rewires in the nucleus
🧪 This directly controls histone methylation (H4K20me1)
🔁 Linking metabolism → chromatin → cell cycle progression
👉 A new layer of regulation: nuclear lipid metabolism as a driver of chromatin state

Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation Nuclear metabolism oscillates during cell cycle progression. Quantitative chromatome proteomics and imaging reveal phase-specific dynamics of PIP5K1A and nuclear PIP2, linking phosphatidylinositol me...

🚨 New paper out!
We show that nuclear metabolism is NOT static, it oscillates with the cell cycle.
🔗 advanced.onlinelibrary.wiley.com/doi/10.1002/...
#epigenetics #metabolism #cellcycle #chromatin

Thanks a lot! We would be happy to explore it together 🤩

Huge work led by Savvas Kourtis, supported by the @erc.europa.eu and @crg.eu

Why are they there?
Do they locally generate metabolites to regulate chromatin?
Could they become new drug targets or disease biomarkers?

These findings highlight how subcellular localization and moonlighting functions can reshape how we think about metabolism.

Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues - Nature Communications Here proteomic chromatome analysis shows metabolic enzymes widely localize to chromatin in cancer in a tissue-specific manner. Nuclear enzymes affect DNA damage/repair and transcription, revealing non...

New paper out in Nature Communications:
www.nature.com/articles/s41...
We found >250 metabolic enzymes on chromatin.
Only ~20 had been reported before.
This means hundreds of metabolic enzymes may have unexplored nuclear roles.

Thanks a lot for the invitation! It was great fun to be there 😊🤩

And then our first PhD student wins the award for Best Thesis of the Year… a wonderful Christmas present 🎄✨

It really makes us feel we’re doing things the right way.

Congratulations, Dr. Natalia Pardo Lorente, so well deserved 👏🎓

@crg.eu @upf.edu @prbb.org

Big congrats to Sara Sdelci @sdelcilab.bsky.social for being as one of the 2025 @embo.org Young Investigators (YIPs), joining 28 outstanding early-career scientists recognised for excellence and originality in the life sciences. She is the eighth CRG scientist to join the prestigious programme!

I am (Sara Sdelci, @crg.eu) very happy to have been selected as an @embo.org YIP! Thank you for selecting our project on how nuclear metabolism shapes cellular proliferation and aging; we are truly excited for the beautiful science ahead!

We did it in cancer cell lines and validated the phenotype in cancer tissues

Not IN, they are compressing the nucleus from the outside ;)

Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects - Nature Communications The authors uncover a mechano-metabolic adaptation where confinement induces rapid mitochondrial relocalization to the nuclear periphery, generating localized nuclear ATP surges that support chromatin remodeling, DNA repair, and cell cycle progression.

Earlier this summer we have published this. Looks like a similar localization of mitochondria in the centre of the nucleus: www.nature.com/articles/s41...

Dr. Jane Goodall has passed away. Her groundbreaking work with chimpanzees and her lifelong dedication to conservation changed how we see and care for our planet.

Happy to share the Biodiversity Cell Atlas white paper, out today in @nature.com. We look at the possibilities, challenges, and potential impacts of molecularly mapping cells across the tree of life.
www.nature.com/articles/s41...

Science: only when you write up the manuscript, you realize what you should have done.

Being open to new ideas: Metabolic Enzymes in the Nucleus as Chromatin Instructors?

Merging
#chromatin
#epigenetics
#metabolism
To understand new cellular rules.

Uncovering how mechanical forces can influence nuclear ATP levels, with downstream effects on DNA damage response and cell cycle progression.

#Mechanobiology #NuclearMetabolism #CellFate #DNArepair #CellCycle

Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects - Nature Communications The authors uncover a mechano-metabolic adaptation where confinement induces rapid mitochondrial relocalization to the nuclear periphery, generating localized nuclear ATP surges that support chromatin...

🚀 We’re excited to share our latest publication in collaboration with Verena Ruprecht's group at @crg.eu

Mechanobiology meets Nuclear Metabolism

www.nature.com/articles/s41...

Fantastic to see this wonderful piece out! Happy to have contributed to this effort 💪

🔬 ¡Buscamos Técnico/a de laboratorio!
📋 Mantenimiento de colonia, procesamiento de muestras y más.
📍 Barcelona
📩+info: hospitalsantjoandedeu.talentclue.com/es/node/1129...

Please RT!

BIG THANKS to everyone involved, especially Toni for his amazing work!

Thanks to our sponsors:
@ageinves.bsky.social
@ERC_Research #ERCStG EPICAMENTE
@crg.eu

Open questions:
Is nuclear metabolism linked to #aging?
Can we reverse the aging process by restoring nuclear metabolism?
What else besides PIP2?

If you are interested in answering these questions, please contact us!

Our study links nuclear PIP2 metabolism to nucleoli structure, histone modification, and cell cycle progression. This provides evidence that nuclear metabolism oscillations can control cell cycle progression, which, when altered, can induce cell cycle arrest and cell senescence.

4) The aberrant localization of PIP2 in the nucleus/nucleoli and the alteration of the nucleoli structure lead to changes in the accumulation of histone marks. For example, H4K20me1 decreases, which subsequently affects proper cell cycle progression.

3) What happens to cells when we interfere with PIP2 metabolism in the nucleus? In addition to preventing cell cycle progression, the structure of the nucleolus is affected and the localization of PIP2 to the Fibrillarin-labeled nucleoli region is altered.

2) We used our lab's gold standard chromatome protocol to study the oscillation of metabolic enzymes on chromatin during the cell cycle. We identified a correlation between cell cycle progression and PIP2 metabolism on chromatin.

1) By integrating FUCCI reporters and classical proliferation/senescent markers, we identified a population of cells, which we termed G0-senescent, that had never before been identified using FUCCI.

Our initial question was: does the cell cycle control the association of metabolic enzymes with chromatin? The short answer is YES!
We adapted the FUCCI system and extensively characterized U2OS FUCCI using HT-microscopy and FACS.