Tumour promotion through the lens of evolution Nature - This Review revisits tumour initiation and promotion in light of clonal diversity and the presence of cancer driver mutations in normal tissues, aiming to understand mechanisms that enable...

Tumor promotion through the lens of evolution

This review grew out of many long conversations, shared ideas, and lively discussions

It has been a real pleasure to think together with Allan, Paul, Eve, and Abel about how tumours develop and how carcinogens shape cancer risk

rdcu.be/fdC2J

How does a cell learn? In our new perspective in @nature.com we propose a model where the properties of the AP-1 family of transcription factors – stress-induced feedback, regulatory combinatorics and cellular memory – encode a mechanism for cellular learning. www.nature.com/articles/s41...

Impact of Chromosomal Instability and Aneuploidy in Cancer Development Somatic human cells contain a diploid genome consisting of 23 pairs of chromosomes. The maintenance of this diploid state is essential across all layers of biological organization, ranging from the ph...

Pleased to share our review on chromosomal instability (CIN) and aneuploidy in cancer, led by Amanda Mennie. Now out @annualreviews.bsky.social!

www.annualreviews.org/content/jour...

Our H1 paper is out #ScienceAdvances:
www.science.org/doi/10.1126/...
@masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in live cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

🚨 Why can’t mammals regenerate limbs like frog tadpoles or salamanders?
In our new paper in @science.org , we show that species-specific oxygen sensing acts as a gatekeeper for initiating limb regeneration 🐭🐸
🔗 www.science.org/doi/10.1126/... #EvoDevo

Why do mammalian cells need PAR, PARP1/2, but yeast does NOT? While studying the impact of PARG inhibition, Isaac in the lab found that extra-PAR sequesters nuclear PAR-binding proteins (XRCC1 and its partners, etc.) into nuclear condensates. biorxiv.org/content/10.6...

Dear all, please see link below for Milano et al, describing a role for BRCA2 (& BRCA1) in the maturation of nascent DNA strand gaps during DNA replication in the presence of PARP inhibitor. Congratulations Larissa and all !

authors.elsevier.com/a/1mtQe3vVUP...

Are you a #Masters student interested in translational
#cancer research, tumor immunology and #CRISPR screens? Then apply to our lab at the @impvienna.bsky.social in Vienna:

www.imp.ac.at/career/open-...

#oncology, #cancerresearch, #CRISPR

Ki-67 shapes the nucleolus by anchoring chromatin via its amphiphilic properties - The EMBO Journal The nucleolus, a membrane-less organelle essential for ribosome biogenesis, adopts variable shapes across cell types and in response to environmental conditions, yet the mechanisms regulating its morp...

Extremely happy to see this wonderful story out! 🔬

Here, we describe how Ki-67 anchors chromatin to nucleoli, leading them to acquire irregular shapes!!! 🫧🧬

This great story was led by Daja Schichler & Yuki Hayashi from the Cuylen Lab @embl.org

link.springer.com/article/10.1...

ChromSMF preprint is out!🚀
tinyurl.com/ChromSMF

We often piece together chromatin regulation layer by layer from separate assays. But this can be limiting!

In @arnaudkr.bsky.social's lab, we developed a method to directly study multiple layers on the same DNA molecule! 🧬

What does this unlock? ⬇️

Many genome-editing tools work by cutting DNA - but the shape of the cut matters. Using genome-wide screening, we show ERCC6L2 is specifically required to maintain repair fidelity at staggered DNA breaks, but not at blunt ends.
www.nature.com/articles/s41...

The Sfeir Lab at MSKCC is hiring a Research Assistant!
We are looking for a motivated scientist with at least 2 years of research experience and a two-year commitment. Learn more about our work here www.sfeirlab.com, and message me your CV and cover letter if interested

Our Lab is searching a postdoc for an exciting ERC-funded project that will start this summer. Apply if you’re passionate about multi-omics, cutting-edge computational approaches, and exploring biological questions across the animal 🪼 🪱 🐞 🦎 🦋 🕷️ tree of life www.biozentrum.unibas.ch/open-positio...

1/New preprint just dropped! 🔥🔥 We investigate how the genome is destroyed in apoptotic cells in a way that prevents DNA fragments from spreading beyond the dying cell 🧬⚰

Done here at @imbavienna.bsky.social & in the super @rcollepardo.bsky.social & Rosen labs:

www.biorxiv.org/content/10.6...

1/n Have you ever wondered whether an amplified oncogene in a patient’s tumor (HER2/EGFR/CCND1/…) is carried on extrachromosomal DNA (ecDNA) vs embedded within a chromosome? Bulk WGS + genome graph can hint, but in practice it’s often hard to resolve. Here is our new preprint.

Most genetic interaction studies measure gene relationships in one condition, but environment can reshape which genes depend on each other.

Today in Molecular Cell, Luke Gilbert & team deliver the first systematic, multi-condition map of genetic rewiring in human cells.

To kickstart this new Bluesky account: We're hiring!
The Bertolin Lab @dundee.ac.uk is looking for a postdoc to work on DNA-end homeostasis & genome stability, funded by Wellcome Trust Award. Exciting science, fantastic environment, founding role in the lab.
Details in the flyer — get in touch! 👇

🧵 Thread: What happens when transcription isn’t properly shut off during mitosis? Our new preprint explores just that 👇
www.biorxiv.org/content/10.6...

Excited to share our latest study in Nature Communications ✨

We map how cells clear DNA damage–stalled RNA polymerase II using a stepwise TFIIH → ubiquitin/VCP mechanism, keeping transcription and genome stability on track.

Paper: www.nature.com/articles/s41...

#Genomics #DNADamage #CellBiology

Our paper on visualising influenza A virus in live cells with single viral RNA resolution is now out in Cell Systems 🥳

For more details about what we did, see the thread about the preprint below ⬇️

Pre-incision structures reveal principles of DNA nucleotide excision repair - Nature Cryo-electron microscopy structures of nucleotide excision repair complexes reveal the roles of the constituent proteins XPA, XPB, XPC, XPD, XPF, XPG and RPA in DNA bubble formation and excision ...

Our great collaboration with the lab of Wei Yang and Kaoru Sugasawa is now online in Nature. We’re glad we could contribute some cell biology to complement this beautiful structural and biochemical work.

www.nature.com/articles/s41...

Transient histone deacetylase inhibition induces cellular memory of gene expression and 3D genome folding - Nature Genetics Acute perturbation of histone acetylation induces changes in chromatin organization that are only partially reversed once the perturbation is removed and are associated with transcriptional memory eff...

TSA treatment may induce cellular and structural memory !! Happy to have been part of this beautiful story, lead by @fpaldi.bsky.social in Giacomo Cavalli's lab. Now published in @natgenet.nature.com 👏.
www.nature.com/articles/s41...

Last days to apply this position in our group! Do join us in this great Institute @crg.eu, with great colleagues, and help shaping the science and culture of our lab!

🧪🧬New preprint We present cryo-EM structures of reconstituted CTCF–nucleosome complexes, showing CTCF dimerization drives nucleosome oligomerization into defined higher-order assemblies. Disrupting CTCF–CTCF interfaces in mESCs reduces looping and impairs differentiation. tinyurl.com/CTCF-nucleos...

Beautiful work, Nibal!!! Congratulations 🥳🥳👏👏

Happy to share the results from my PhD thesis, in which we uncovered a function for FANCD2 on maintaining fork stability upon re-replication, now out in Nat. Commun!

📖 Read the full story here: 

www.nature.com/articles/s41...

Thanks to everyone who supported me along the way!

Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stability Marmolejo et al. uncover transcription condensate dynamics at histone locus bodies across the cell cycle. These dynamics are governed by cell cycle (DDK and CDK1/2) and checkpoint (ATR-CHK1) kinases t...

I am excited to share the first paper from the Saldivar lab! A special thank you to all who contributed.

Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stability: Molecular Cell www.cell.com/molecular-ce...

Don't miss the opportunity to attend exciting virtual talks from leading scientists in genome integrity, provided by the @genomeiinetwork.bsky.social! Next one is in a few days 👇

PAF15–PCNA exhaustion governs the strand-specific control of DNA replication Nature - PCNA–PAF15 has a key role in determining replisome dynamics during genome replication and protecting against genome instability.

Dear Colleagues,

Delighted to share that our first lab paper is now published in Nature.!!! 😊😊😊

We identify a previously concealed “safety limit” in DNA replication program: cells intrinsically rate-limit PCNA via PAF15, shaping strand-specific and global replication dynamics.

👉 rdcu.be/e1bBt

Site-saturation functional screens identify PALB2 missense variants associated with increased breast cancer risk - Nature Communications The impact of variants of uncertain significance (VUS) on protein function and cancer risk remain unclear. Here, the authors focus on the functional impact of VUS of the PALB2 gene and identify defect...

We present a site‑saturation functional screens of missense variants in the high-risk breast cancer susceptibility gene PALB2. Damaging missense variants confer a high risk of breast cancer! Thanks to all co-authors and to KWF for financially supporting our work!

www.nature.com/articles/s41...