Spike-in enhanced phosphoproteomics uncovers synergistic signaling responses to MEK inhibition in colon cancer cells Nature Communications - Kinase inhibitors are key in cancer therapy, but resistance limits their efficacy. Here, the authors develop SPIED-DIA, a phosphoproteomics method enhancing detection of key...

Read our new paper in Nature Comms:
SPIED-DIA = spike-in enhanced DIA phosphoproteomics
-> Boosts detection of key phosphosites
-> Reveals JNK activation upon MEK inhibition in CRC cells
-> Dual MEK/JNK targeting impairs growth
🔗 rdcu.be/enPF2
#proteomics #DIA #cancer

Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets Pancreatic islets are highly specialized tissue compartments that regulate metabolism, and their dysfunction contributes to diseases such as prediabetes and diabetes. Characterizing islet morphology a...

Excited to share our preprint on multiplexed DVP! mxDVP extends CODEX to DVP family, revealing spatial heterogeneity in human pancreatic islets with 12 endocrine sub-types including rare polyhormonal cells. Fantastic team effort #LundbergLab biorxiv.org/content/10.1101/2025.04.27.650857v1

A tissue-specific atlas of protein–protein associations enables prioritization of candidate disease genes - Nature Biotechnology Protein–protein interactions specific to 11 individual tissues are presented in a comprehensive resource.

Our work on deriving protein-protein (functional) associations across different human tissues from proteomics data is now published. There is quite a lot in this paper, so I will just touch on a few highlights:
www.nature.com/articles/s41...

📢Register for the Berlin symposium #Biology_of_Health Towards a Molecular Approach to Preventive Medicine!
🗓️ 19-20 May 2025
🎤 Inspiring line-up of speakers! Keynotes by Amos Tanay, Elvira Mass, Aleksejs Sazonovs, and Nikolaus Rajewsky
✏️Register & full program: rb.gy/kos81
📌 Registration: 12 May 2025

Alternative splicing (AS) is common, but its impact on the proteome remains debated, especially in primary cells.

What determines which AS isoforms result in abundant proteins ?

We find that proteoform-specific ...

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www.biorxiv.org/content/10.1...

What happens to all those alternative transcripts?
👉 Many are translated.
👉 Proteolytic processing adds even more diversity.
Integrated mRNA & protein isoform landscape now out on bioRxiv!
🔗 doi.org/10.1101/2025...
#proteomics #transcriptomics #systemsbiology