Exactly 👍

Evolution of the ribosomal exit tunnel through the eyes of the nascent chain The ribosomal exit tunnel is a universally conserved feature of the large subunit that directs the nascent polypeptide chain into the cellular environment and is involved in co-translational folding, ...

Just in time for 2026! 🎆 Presenting a nascent-centric view on the shape of the ribosomal exit tunnel topology, based on MD-derived occupancy maps for 55 distinct ribosomes. 🧶🧬🖥️
📄 Read the preprint: biorxiv.org/content/10.6...

#StructuralBiology #Ribosome #CryoEM #Evolution #Biophysics

This work was also our first time doing membrane localization (way to go Cassidy)! And so proud of Bella’s gorgeous Paenibacillus tree showing the distribution of the transmembrane containing Spo0B! Also it’s the lab’s first paper without ribosomes 🫨, but don’t worry - the ribosomes made the Spo0B!

Nice, Thanks

Thank you, I really appreciate it. It appears to work partially in yeast, so it would be great to test it in plants.

This sequence optimization technique is also included in the overall sequence optimization tool available on our server: opt.williams.edu
(See our publication: www.sciencedirect.com/science/arti...)

In this study, we highlight the importance of the base composition of the first codons for protein expression. An increased abundance of adenine (A) and a decrease in guanine (G) enhance expression and improve mRNA stability. The mediator of this effect is the ribosome.

Base composition at the start of the coding sequence controls the balance between translation initiation and mRNA degradation in E. coli Abstract. Protein synthesis efficiency is highly dependent on the messenger RNA (mRNA) coding sequence. Furthermore, there is extensive evidence of a corre

Our latest paper on protein expression and mRNA stability is out!

academic.oup.com/nar/article/...

Check out how to increase your protein output with a simple trick.

Congratulations to first author @Anna Lipońska, it was a great collaboration with the Aalberts group.

#RNAsky #ribosome #EGM

Thanks Zeynep

Uridine as a potentiator of aminoglycosides through activation of carbohydrate transporters Uridine boosts aminoglycoside treatment efficiency against antibiotic-susceptible as well as antibiotic-resistant E. coli strains.

So excited our antibiotic potentiation story is out 🤩 Led by the extraordinary @manonlang.bsky.social with @fox-science.bsky.social & @amazeld.bsky.social +amazing collaborators @immunobladder.bsky.social @imaneelmeouche.bsky.social 🦠 We believe it can make a difference in #AMR infections!

Congratulations great story

A useful toolkit 🧰⚙️

Thanks for the great meeting.
It was so nice to see everyone and enjoy great science. 🔤❤️

Title, authors and abstract from link

In a remarkable new paper, Qureshi and Duss use multi-colour single-molecule fluorescence microscopy to directly and simultaneously track transcription, translation & coupling 3/n
www.nature.com/articles/s41...

Codon influence on protein expression in E. coli correlates with mRNA levels - Nature In-depth analyses of protein expression studies are used to derive a new codon-influence metric that correlates with global protein levels, mRNA levels and mRNA lifetimes in vivo, indicating tight cou...

The second method does the same but also
optimizes all other codons using the 44 best codons identified in our
previously published efficiency codon metric (doi.org/10.1038/natu...). The last method (AG29G) follows a similar
approach but utilizes only the 29 best codons.

Codon influence on protein expression in E. coli correlates with mRNA levels - Nature In-depth analyses of protein expression studies are used to derive a new codon-influence metric that correlates with global protein levels, mRNA levels and mRNA lifetimes in vivo, indicating tight cou...

The first
optimization method (AGhead) focuses solely on the first codons to increase the
ratio of adenosine to guanosine.

The manuscript comes with a web server, opt.williams.edu, that allows for the prediction of expression for
a given DNA sequence and proposes three types of optimization.

I am very happy to present our new manuscript in collaboration with the Aalbet and Hunt Labs on DNA sequence optimization for high expression in Escherichia coli as well as in in vitro translation assays (doi.org/10.1016/j.jm...)

Super happy to be with the ABC family

It's ABC protein, NOT transporter! We are an inclusive bunch! ❤️
Gregory Boel introducing the morning speakers! #ABC2025

We are all the same super family 🥰

Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome ... It can be difficult to find rare transcripts when sequencing a transcriptome. Here the authors show adaptive sampling on direct RNA runs to increase the likelihood of finding less frequent ones while ...

Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome www.nature.com/articles/s41... #jcampubs

RiboGraph: An interactive visualization system for ribosome profiling data at read length resolution bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

check it out!
RiboGraph: An interactive visualization system for ribosome profiling data at read length resolution

🔎 microprotein
🧬 + 🖥️

www.biorxiv.org/content/10.1...

Thanks to @onamy.bsky.social for the code 🙏

www.biorxiv.org/content/10.1...
Time resolution in cryo-EM using a novel PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling.
Just accepted by CELL as a resource article.

Hi everyone,
We are new on Bluesky 😀. We are a group of microbiologists 🧫🔬 interested in mRNA translation mechanisms and regulation.
We wish you a wonderful year in 2024 🥳🍾 and will be back soon with more news.

Global regulation via modulation of ribosome pausing by EttA https://www.biorxiv.org/content/10.1101/2023.10.17.562674v1 Having multiple rounds of translation of the same mRNA is a key feature of the Central Dogma that cr

Global regulation via modulation of ribosome pausing by EttA www.biorxiv.org/content/10.1101/2023.10....