Electrostatic properties of disordered regions control transcription factor search and pioneer activity - Nature Communications This work describes how positive charge in the peptide chain flanking the DNA-binding domain of transcription factors speeds up their search on free and chromatinized DNA, helping to explain why some ...

How do electrostatic forces impact TF search ? In joint effort with @beatfierz.bsky.social , tour-de-force from @simsakong.bsky.social who single-handedly combined in vitro SPT on DNA & chromatin, in cellulo SPT, ChIP-seq and ATAC-seq www.nature.com/articles/s41...

I’m excited to share that my PhD work is now published in @natcomms.nature.com! Big thanks to @davidsuter.bsky.social & @beatfierz.bsky.social for your guidance throughout this work. We show that electrostatic differences in disordered regions control TF search and chromatin invasion.

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Electrostatic properties of disordered regions control transcription factor search and pioneer activity - Nature Communications This work describes how positive charge in the peptide chain flanking the DNA-binding domain of transcription factors speeds up their search on free and chromatinized DNA, helping to explain why some ...

TFs search are governed by many parameters, what about electrostatic forces? @simsakong.bsky.social led a multi-scale study on Sox2 revealing how electrostatic interactions influence its search. In close collaboration with @davidsuter.bsky.social. Congratulations Sim 🎉
www.nature.com/articles/s41...

Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexes Single-molecule experiments show that active conformation formation controls chromatin ubiquitylation kinetics by variant PRC1.

Our new study of chromatin ubiquitylation by variant PRC1 on the single-molecule scale: We visualize directly how vPRC1 ubiquitylates neighboring nucleosomes during a single binding event, showing a potential mechanism how H2Aub domains are established.
www.science.org/doi/10.1126/...

Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexes Single-molecule experiments show that active conformation formation controls chromatin ubiquitylation kinetics by variant PRC1.

If you always wanted to know the kinetics of how the Polycomb enzymes modify chromatin, we are thrilled to share our work!!! 🎉📄🧬🔬

Thank you, @beatfierz.bsky.social for all the guidance as well as the @lcbm-epfl.bsky.social group members for all the support!
doi.org/10.1126/scia...

Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexes Single-molecule experiments show that active conformation formation controls chromatin ubiquitylation kinetics by variant PRC1.

Check out our new study of chromatin ubiquitylation. @alexateslenko.bsky.social visualized directly at single molecule scale how vPRC1 ubiquitylates neighboring nucleosomes. These results suggest potential mechanism on how H2Aub domains are established. 👇👇👇
www.science.org/doi/10.1126/...

Congratulations @matteodoudin.bsky.social for this PhD fellowship 🥳🎉. Stay tuned for his next achievements about nucleosome depletion.

#SIRT7 is a histone deacetylase with highly specific activity on #chromatin substrates.
We just published mechanism-based #cryoEM structures of #SIRT7 on nucleosomes to understand its activity 👇
www.nature.com/articles/s41...
(1/8) #ChemBio #ChemSky