Together, these findings uncover a novel facet of phage–host interactions by showing that a regulated 3D orchestration of phage genome organization takes place during infection. Our study provides the first evidence that such a level of structural dynamics in a phage-bacteria system exist.

Analyses of interactions patterns between the two genomes demonstrate that phage tends to interact with low active region of host genome suggesting a localization of the phage in the middle of the nucleoid; probably to better hijack host cellular machinery.

We also characterise phage genomes dynamics that undergo an important decondensation while entering into the cell and a rapid structuration of its early genes cluster potentially involving a NAP-like factor.

We applied time series HiC and combined it with transcription data to study the dynamic of phage genome from inside its capsid till the end of its infection cycle.
Infection leads to a local destructuration of host genome in link with transcription arrest while its global architecture remains.

💥 alert article 💥
New preprint from the lab !!! 🥳
In this work, we explore the 3D genome architecture of the virulent phage PAK_P3 during its infection cycle in P. Aeruginosa. We unveil a highly dynamic structuration as well as specific interactions patterns.

www.biorxiv.org/content/bior...

Les phages à large spectre, omniprésents dans les écosystèmes Les phages, les virus des bactéries, représentent les entités génétiques les plus abondantes de notre environnement.

#ResultatScientifique 🔎| Et si les phages, ces virus des bactéries, pouvaient infecter plusieurs espèces à la fois et remodeler l’écologie microbienne ? 🦠
✍️ Martial Marbouty
📕 @natmicrobiol.nature.com | buff.ly/1R9kqjv

Thanks Meren !! Coming from you … I am honoured !!

New paper!
We generated and/or (re)analyzed ~100 #metagenomics Hi-C datasets to provide a comprehensive analysis of bacterial and viral metagenomes across VERY different environments.
Congratulations to all authors especially Amaury Bignaud and @mmarbout.bsky.social for the huge work!
#metaHiC

Our work show that phages with a broad host ranges are common across ecosystems. It also highlights the interest of using HiC to study single metagenomic samples.
Congrats to all authors !! 🎉

We then show that among the phages with an assigned host, a substantial proportion of them interact with several, and sometimes distantly related, hosts.

We have been able to reconstruct numerous good quality viral genomes up to 1Mb from diverse families such as Megaviricetes or Caudoviricetes.

We then apply our approach on public and newly generated metaHiC datasets (111 in total) from different ecosystems to explore the virosphere of these communities.

GitHub - koszullab/metaTOR: Metagenomic binning based on Hi-C data Metagenomic binning based on Hi-C data. Contribute to koszullab/metaTOR development by creating an account on GitHub.

We first used a synthetic community composed of various microorganisms and mobile genetic elements to develop a new version of our MetaTOR pipeline allowing to reconstruct virus genomes and identify their hosts in microbial communities.

github.com/koszullab/me...

Phages with a broad host range are common across ecosystems - Nature Microbiology Proximity-ligation-based sequencing from 111 samples and 5 environments reveals that a substantial proportion of phages infect multiple species.

You like phages ? In this publication, we use metaHiC and our new version of the MetaTOR pipeline to challenge the traditional view of phages with a narrow host range.
@rkoszul.bsky.social @natmicrobiol.nature.com @cnrs.fr @institutpasteur.bsky.social

www.nature.com/articles/s41...

Comment détourner les bactériophages à des fins thérapeutiques. Dans nos organismes, les relations entre bactéries et phages tissent un réseau complexe dont l'analyse ouvre des thérapies innovantes, notamment pour réguler le microbiote intestinal.

Reportage de @celineloozen.bsky.social > Itw de Martial Marbouty, chercheur CNRS au laboratoire Régulation Spatiale des Génomes de l'Institut Pasteur sur l’étude des interactions phages-bactéries tinyurl.com/m9uxwjct #ScienceCQFD

Bactériophages, une épopée thérapeutique Découverts au début du 20e siècle, les virus bactériophages ont été rapidement délaissés dans la lutte antibactérienne au profit des antibiotiques. Mais face à la crise de l’antibiorésistance, ces "ma...

#Podcast du jour. Virus bactériophages : découverts début XXe s, ils ont été délaissés dans la lutte antibactérienne au profit des antibiotiques. Face à l’antibiorésistance, les bactériophages pourraient-ils faire leur grand retour ? tinyurl.com/yoat4jcu avec Anne Chevallereau et Jérôme Gabard

Great science, great discussions, amazing people… a real pleasure to be here !!