ER-derived #Caveolin-coated vesicles transport newly synthesized #cholesterol to the plasma membrane. New study from Ming Sun, Yuan-Bin Liu, Pu-Yu Sun, Bao-Liang Song and colleagues (Wuhan University): rupress.org/jcb/article/...

#Biochemistry #Membrane #lipid #Trafficking #ER_literature

Expansion microscopy Nature Reviews Methods Primers - Expansion microscopy enhances conventional imaging by physically enlarging samples within a hydrogel matrix, surpassing diffraction limits. In this Primer, Vo et...

📷 Expansion microscopy turns 11 — and our comprehensive Primer is now live in
@natrevmethodsprimers.nature.com
! A decade of ExM, 1000+ papers, and still accelerating. Co-authored with the father of the field,
@eboyden3.bsky.social
, + Ha Vo & Rong Xu. 📷 Free to read: rdcu.be/fdQPH

1/ 🔬🧑‍💻 Tired of going back and forth between GUIs and code for your 3D image analysis needs? Would you like to keep your focus in one place (notebook), without sacrificing interactivity? Here’s a tool that can help!

I am so excited to share our new findings with you! We provide the structural evidence for a direct protein-to-DNA information pathway, showing how a bacterial enzyme 'reads' its own structure to 'write' DNA. www.science.org/doi/10.1126/...

omg mind blown. thanks for sharing!

these are my favorite kind of papers - so cool🤩

A nature-inspired nanoplatform for multi-protein targeted degradation via the autophagy-lysosome pathway By elucidating the lysosomal sorting mechanism of extracellular vesicles, Tong et al. developed a nature-inspired nanoplatform for targeted protein degradation. Featuring a modular design with multiple engineerable epitopes, the platform supports both plug-and-play antibody conjugation for individual targets and simultaneous degradation of multiple proteins via multiplexed antibody display.

A nature-inspired nanoplatform for multi-protein targeted degradation via the autophagy-lysosome pathway

Receptor-ubiquitination-targeting antibody-drug conjugates for enhanced endocytosis and lysosomal delivery Zhuang et al. address limited antibody-drug conjugates (ADCs) internalization by developing ubitaADCs that harness receptor ubiquitination. By engaging membrane E3 ligases, ubitaADCs promote receptor endocytosis and lysosomal trafficking, enhancing intracellular payload delivery and supporting a modular strategy applicable to multiple tumor-associated receptors.

Receptor-ubiquitination-targeting antibody-drug conjugates for enhanced endocytosis and lysosomal delivery

Lysosome-targeting chimeras enable targeted protein degradation Bozhao Li et al. review lysosome-targeting chimeras (LYTACs) as a transformative strategy for degrading extracellular and membrane proteins. They outline receptor-guided design principles, endocytic routing mechanisms, and translational challenges, highlighting how LYTACs expand targeted protein degradation beyond the proteasome.

Lysosome-targeting chimeras enable targeted protein degradation

Wang et al. show that migrasome formation requires tetraspanin 4 palmitoylation regulated by DHHC6 and PPT1. This modification drives tetraspanin 4 clustering with cholesterol to build migrasomes.
rupress.org/jcb/article/...

#Migration #motility #Development #Organelles #Biochemistry

A series of spontaneously blinking dyes for super-resolution microscopy - Nature Methods A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...

Out in @natmethods.nature.com: More dyes. They work. Quite well. And blink. Pick the one that fits your target, your technique, and your labeling density. With too many collaborators and institutes to list, but anchored at @hhmijanelia.bsky.social www.nature.com/articles/s41...

A surface rendered view of an endosome. The endosome surface is rendered in blue with dimples on it. In specific regions of the endosomal surface, clusters of proteins are seen as aggregates of blobs, coloured in yellow and red.

🚨New paper out today in @acs.org ACS Nano, showing how expansion microscopy can visually quantify the cell surface receptor turnover mediated by signalling & receptor organising machineries on the surfaces of endosomes, some of the smallest organelles in cells. 🧪 🔬 1/ pubs.acs.org/doi/10.1021/...

Visualizing suborganellar lipid distribution using correlative light and electron microscopy - Nature Cell Biology Lennartz et al. introduce a correlative light and electron microscopy workflow, Lipid-CLEM, combining near-native lipid probes and on-section labelling via click chemistry. Lipid-CLEM quantitatively a...

☕ @mathilda95.bsky.social @nadlerlab.bsky.social & co introduce a correlative light and electron #microscopy workflow, Lipid-CLEM, combining near-native #lipid probes and on-section labelling via click chemistry. Lipid-CLEM quantitatively analyses lipids in membrane nanodomains.
bit.ly/4moYLJH

We're turning 29 episodes old! Learn from @erinweisbart.bsky.social and @bethcimini.bsky.social about Pooled Cell Painting, Cell Painting's version of Optical Pooled Screening. It's high-throughput heaven!

Image of a calcium calibration curve, calcium concentration traces of individual cells and lifetime images of cells

📣 New preprint alert! Co-authored by @spalaciosm.bsky.social and @andreacaldarola.bsky.social :
Quantitative imaging of calcium dynamics with a green fluorescent biosensor and fluorescence lifetime imaging
www.biorxiv.org/content/10.6...

Ming Sun, Yuan-Bin Liu, Pu-Yu Sun, Bao-Liang Song et al. show that newly synthesized #cholesterol is transported from the endoplasmic reticulum to the plasma #membrane via specialized COPII-independent vesicles. rupress.org/jcb/article/...

#ER_literature #lipids #trafficking

Now out in its final form in @jacs.acspublications.org: Reaction pathways & photoreaction yields of bifunctional lipid probes. The numbers that explain why we spend so much time optimizing protocols for cellular assays. 😉 pubs.acs.org/doi/10.1021/...

csmb 2026: grateful for all the cool science, great people, and amazing scenery

it was great meeting you and learning about your really cool work with cytonemes!

Great read!

26-year-old now 'cancer-free' thanks to new colorectal cancer treatment A 26-year-old is now "cancer-free" after receiving a new treatment for colorectal cancer.

Good news story: a 26yo with stage 3 colorectal cancer received immunotherapy & avoided months of chemo & radiation - she even ran a 5K during treatment

Cancer care is evolving toward safer, personalized treatments driven by immune innovations & precision medicine 🧬

abcnews.com/wellness/sto...

im so lucky to be able to surround myself with cell biology and scientists, seeing the result of people's hard work and rigorous questioning is seriously inspiring

Cool!!

Thank you!

Limitations of serial cloning in mammals - Nature Communications Here they show that extended serial somatic cell cloning imposes a threefold increase in de novo mutations compared to natural reproduction, progressively reducing birth rates and ultimately limiting ...

Well here's an answer to a question I hadn't thought of asking www.nature.com/articles/s41...

A high-affinity split-HaloTag for live-cell protein labeling Nature Communications - Lin and colleagues present high-affinity split-HaloTag pairs for protein tagging and multiplexed labelling. This versatile system allows protein visualisation with diverse...

A high-affinity split-HaloTag for live-cell protein labeling. And much more.
www.nature.com/articles/s41...

Omg !!

CD47 inhibits phagocytosis through Vav dephosphorylation Miller et al. demonstrate that the CD47 signaling pathway prevents phagocytosis through dephosphorylation of the Rac GEF Vav. Cancer cells and synthetic ta

Second, Wyatt Miller clarifies the downstream signaling pathway that the "Don't Eat Me" signal CD47 uses to stop phagocytosis. A very satisfying answer to a major question we've had since I started my postdoc, with lots of interesting implications doi.org/10.1083/jcb....

🚨📰Now online in its full form www.nature.com/articles/s41...

I think im never going to get over super resolution microscopy