This image shows details about the article linked in the post. It was published in the Nature Portfolio Journal Science of Food, and the title is, "Proactive surveillance of foodborne bioactives by integrated dietary exposure and urinary excretion assessment."

To help identify possible dietary exposure scenarios, authors developed & validated a high-throughput platform to simultaneously screen for 331 compounds & quantify 214 analytes. They detected banned substances in products & did exposure & excretion simulations. 🚫💊 🧪

www.nature.com/articles/s41...

172 - FIFA's Role in Supporting Player Health, Clean Competition, and Sport Integrity in Soccer - Carlos López Tiboni - PCC - Global Anti-Doping Research Grants for Scientists and Researchers 0.75x 1x 1.25x 1.5x 2x 0:0044:15 172 – FIFA’s Role in Supporting Player Health, Clean Competition, and Sport Integrity in Soccer – Carlos López Tiboni Apple PodcastsGoogle PodcastsPlayer EmbedShare Le...

He details the logistics and collaboration required to execute anti-doping education and testing for a major sporting event like the FIFA World Cup taking place in North America this summer.

Listen to the full episode here:
bit.ly/4ulEkAb

In our most recent podcast episode, we talk with Carlos López Tiboni, Senior Anti-Doping Manager at the Fédération Internationale de Football Association (FIFA). Carlos shares how FIFA interacts with different football leagues, clubs, and other sports organizations globally to support clean sport.

Their new protocol addresses issues with current gas chromatography-based methods and is better suited for polar substances like AICAR.

Both Dr. Michael Polet and Dr. Peter Van Eenoo have previously received PCC funding for their research.

#antidoping #cleansport #research #science #chemistry

Rapid Analysis of Urinary AICAR by Liquid Chromatography–Isotope Ratio Mass Spectrometry 5-Aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) is prohibited in sports according to the World Anti-Doping Agency (WADA). Currently, AICAR concentrations are measured during the initial testing procedure, and urine samples showing elevated levels are flagged as suspicious and forwarded for confirmation. Here, gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS) is employed to distinguish endogenous from exogenous AICAR, as synthetic AICAR exhibits a different carbon isotope ratio (CIR). However, a GC-C-IRMS method comes with multiple challenges as AICAR is very polar, making it particularly difficult to analyze using GC. It is clear that there are a number of issues related to the existing protocol, and efforts should be made to develop new, more effective, and less expensive approaches. For polar substances, liquid chromatography–isotope ratio mass spectrometry (LC-IRMS) provides a more straightforward approach. There is no need for derivatization, AICAR can be directly analyzed with LC-IRMS, and many GC-C-IRMS related issues are avoided. LC-IRMS also allows condensing of the workflow and lowering of the turnaround time. In this study, a rapid protocol for AICAR CIR determination was developed, employing solid-phase extraction and 2D-LC cleanup prior to LC-IRMS analysis. Etiocholanolone and androsterone were used as endogenous reference compounds, and the method was fully validated according to WADA regulations. Reference population (n = 50) thresholds, used to declare an adverse analytical finding (AAF, i.e., a positive AICAR sample), were determined (6.9‰ and 5.8‰ for AICAR–etiocholanolone and AICAR–androsterone, respectively) and found to be approximately 2‰ lower than those previously reported using GC-C-IRMS, facilitating AAF identification.

AICAR is a metabolic modulator banned in sport at all times. It is also produced endogenously, & carbon isotope ratios (CIR) can distinguish endogenous vs. administered AICAR. Researchers developed & validated a rapid protocol to determine the CIR of AICAR via LC-IRMS.
pubs.acs.org/doi/10.1021/...

In our recent podcast episode we talk with Holly Shick, Chief Ethics & Compliance Officer at the USOPC, about how she found her role, and how she and her team have transformed the relationships between the USOPC & its national governing bodies to advance clean sport & protect athletes
bit.ly/4mfbn5T

This image shares details about the article linked in the post it was published in the American Journal of Physical Medicine & Rehabilitation. The title of the article is "Performance Enhancement in Paralympic Athletes: Blood Doping, Boosting, and Beyond."

Performance-enhancing substances & methods may have different effects & risks for Para athletes with spinal cord injury or other conditions. Authors reviewed the evidence on the effects & risks of blood doping & other methods in Para athletes & shared research gaps.
pubmed.ncbi.nlm.nih.gov/41534108/

This image shows details about the paper linked in the post. It is published in the journal Drug Testing And Analysis. The title of the article is, "Manhole Sampling at Gyms and Sports Centers in Denmark: A Pilot Study Using Wastewater Analysis as a Tool for Monitoring the Use of Performance-Enhancing Drugs".

Authors studied use of wastewater sampling near gyms & sports centers to investigate use of performance & image-enhancing drugs in recreational athletes. Illegal anabolic agents were detected. This sampling method could complement traditional anti-doping approaches.
pubmed.ncbi.nlm.nih.gov/41386665/

This image provides details about the article linked in the post. It was published in the journal BMJ Open Sport & Exercise Medicine. The title of the article is, "International elite Para athletes' perspectives on anti-doping: what works, what doesn't and what's next?"

In a study assessing how international elite Para athletes perceive #antidoping procedures, authors found 27% of respondents never received anti-doping education and >50% were never tested. Further work is essential to meet the needs of elite Para athletes.

bmjopensem.bmj.com/content/11/4...

171 - Strengthening Ethics and Compliance Accountability in U.S. Olympic and Paralympic Communities - Holly Shick, JD - PCC - Global Anti-Doping Research Grants for Scientists and Researchers 0.75x 1x 1.25x 1.5x 2x 0:0033:23 171 – Strengthening Ethics and Compliance Accountability in U.S. Olympic and Paralympic Communities – Holly Shick, JD Apple PodcastsGoogle PodcastsPlayer EmbedShare Le...

Check out our latest podcast episode featuring our newest member of the Board of Governors, Holly Shick. Holly represents the USOPC, where she is the Chief Ethics & Compliance Officer. We talk about Holly's career, her role in the Milan-Cortina Games, and preparations for LA28.

bit.ly/4mfbn5T

Senior author Dr. Peter Van Eenoo has previously received PCC funding to support his research.

#cleansport #antidoping #drugtesting #steroids #chemistry #analyticalchemistry #science #research

Simple and sensitive cellulose paper-based microextraction of 25 steroid esters from human serum/plasma for doping control purposes - Analytical and Bioanalytical Chemistry Blood is increasingly used as a biological matrix in the doping control field; however, the limited volume typically available for analysis poses a significant challenge, particularly when multiple te...

To help address the issue of limited blood sample volumes for multiple anti-doping tests, researchers developed and validated an easy and efficient microextraction protocol that can be used to extract 25 steroid esters from only 20 µL of serum or plasma.

link.springer.com/article/10.1... #science

Don’t miss our latest podcast episode with Dr. Neil Robinson, Head of the Science & Medical Department at the International Testing Agency. He shares the process of sample storage and reanalysis, what prompts samples to be reanalyzed, and the impacts of these efforts on clean sport
bit.ly/4lQ470e

The image provides details about the paper linked in the post. The title of the article is, "Performance-Enhancing Effects of Inhaled Medications: Implications for Heart, Muscle Function, and Doping Detection in Athletes". It was published in J Funct Morphol Kinesiol.

This recent review examines how inhaled medications, like bronchodilators, can impact the cardiovascular and muscular systems, discusses relevant doping cases, describes current detection methods, and presents ongoing challenges and knowledge gaps.

pmc.ncbi.nlm.nih.gov/articles/PMC...

As we close out Women’s History Month, we’d like to recognize the wonderful women in leadership roles at the PCC. We would not be a premier funder of world-class anti-doping research without their continued guidance and expertise. Thank you for leading by example and for supporting our mission!

Evaluation of Different Detection and Identification Methods of Intact Tetrahydro‐Methyltestosterone Sulfate Metabolites in Doping Control The micro-scale synthesis of the six out of eight possible tetrahydro-methyltestosterone sulfate metabolites was performed, and the synthesized materials were used as analytical standards. The analys...

This study found that certain sulfate metabolites increased the window of detection for methyltestosterone use. More work is needed to enhance methods for unambiguous identification before the metabolites can be routinely included in initial testing procedures. 🚫💊 🧪
pubmed.ncbi.nlm.nih.gov/41834284/

She found that several indirect biomarkers are linked to GH administration, which calls for further validation & the development of a general analytical protocol that could be implemented in anti-doping labs.

Read more about Dr. Buisson’s findings here:
bit.ly/4v1f1oa

Up next in our Women’s History Month research spotlight is Dr. Corinne Buisson. Dr. Buisson conducted a PCC-funded pilot study focused on discovering new biomarkers related to growth hormone (GH) to create an innovative screening strategy to indirectly detect GH micro-doses.

Info about the paper linked in the post

Authors showed capillary blood analysis can help interpret #antidoping results for 2 types of drugs with complex pharmacokinetics. Complementary blood analysis can help determine in-competition vs out-of-competition use & provide more consistent detection. 🚫💊 🧪

pmc.ncbi.nlm.nih.gov/articles/PMC...

Her research provided greater understanding of the dissociation pathways of Beta2 agonists and other drugs of that class. Dr. Patrick’s findings may aid in the development of MS/MS methods and efforts toward predicting MS/MS patterns.

Read more about Dr. Patrick’s research here:
bit.ly/4t194We

Our next feature for Women’s History Month is Dr. Amanda Patrick. Dr. Patrick received funding for her project which developed quick and sensitive methods to differentiate between therapeutic use and performance-enhancing use of Beta2 agonists.

170 - Enhancing Anti-Doping Through Sample Storage and Reanalysis - Neil Robinson, PhD - PCC - Global Anti-Doping Research Grants for Scientists and Researchers 0.75x 1x 1.25x 1.5x 2x 0:0048:40 170 – Enhancing Anti-Doping Through Sample Storage and Reanalysis – Neil Robinson, PhD Apple PodcastsGoogle PodcastsPlayer EmbedShare Leave a ReviewListen in a New Win...

In our recent podcast episode, we spoke with Dr. Neil Robinson, Head of the Science & Medical Department at the ITA. He discussed anti-doping innovations, including the logistics of long-term sample storage & reanalysis & what can be gained from storing samples as the field advances.
bit.ly/4lQ470e

Co-authors Drs. Lena Ekström, Olivier Salamin, and Raul Nicoli have previously received PCC funding for their research.

#biomarkers #steroids #cleansport #antidoping #research #science @ki.se

This image shows details about the paper linked in the post. The title of the article is "Variation of the steroid profile in relation to training in women and its importance for anti-doping testing," and it was published in the journal Frontiers in Physiology.

Interpreting the steroid modules of the ABP can be complex in female athletes due to hormonal fluctuations. This study examined urinary & circulatory steroids in female athletes and controls to determine how training & sweat production impact steroid biomarkers.

www.frontiersin.org/journals/phy...

Co-authors Dr. Mario Thevis and Dr. Thomas Piper have previously received PCC funding to support their research.

#antidoping #cleansport #research #science #AI #machinelearning

Detection of sample swapping in anti-doping investigations using machine learning - Scientific Reports Scientific Reports - Detection of sample swapping in anti-doping investigations using machine learning

It's hard to detect when athletes swap a urine sample taken post-doping w/ their own pre-doping urine. Authors developed a similarity detection framework using machine learning to automatically identify reused/identical urine samples in the Athlete Biological Passport.
www.nature.com/articles/s41...

They have published studies on topics such as the athlete biological passport, advances in sample preparation, masking agents, and more. Visit our website to learn more about each of these researchers and the work they've contributed to the field of anti-doping.
cleancompetition.org/funded-proje...

For our next Women’s History Month feature, we want to spotlight our outstanding female fellows. The PCC’s Fellowship Program supports qualified scientists who demonstrate a strong interest in & potential for contribution to anti-doping science. These researchers represent nations across the globe.

Her project contributes to the integrity of clean sport by proposing the inclusion of these biomarkers for the detection of testosterone abuse.

Our next Women’s History Month feature is Dr. Yvette Dehnes, Director of the Norwegian Doping Control Laboratory at Oslo University Hospital. Dr. Dehnes received PCC funding for a research project which found that testosterone administration lowers plasma hepcidin & increases reticulocyte ALAS2 mRNA