Wow, the immune system is merciless with everything that is not self!

Mesmerizing light sheet imaging of neutrophils swarming at a yeast target!
The neutrophils were labeled with SPY650-DNA (yellow) and a calcium dye (blue).

This movie is part of the recent manuscript by @strickland-evelyn.bsky.social that we highly recommend reading!

www.biorxiv.org/content/10.6...

Development of the fluorescent probe CenSpark for labeling centrioles and cilia - Nature Chemical Biology CenSpark is a dual-ligand fluorescent probe binding simultaneously inner and outer microtubule sites, a configuration unique to doublet and triplet microtubules, enabling selective live imaging of cen...

Very cool probe to label doublet MTs and therefore centrioles across different species and set ups (from live imaging, STED and expansion)!😍

www.nature.com/articles/s41...

CenSpark is out !

Congrats Gönczy team et al.

What a probe! Congratulations @cepourroy.bsky.social!
We're happy to share that we re working hard on making CenSpark probes available to the centriole aficionados in the coming weeks!

Development of the fluorescent probe CenSpark for labeling centrioles and cilia - Nature Chemical Biology CenSpark is a dual-ligand fluorescent probe binding simultaneously inner and outer microtubule sites, a configuration unique to doublet and triplet microtubules, enabling selective live imaging of cen...

CenSpark story is now published in @natchembio.nature.com. We engineered a dual-ligand fluorescent probe to selectively "light up" centrioles & cilia. Massive thank to Pierre Gönczy, @lreymond.bsky.social and @ghatzopoulos.bsky.social for the guidance. Check it out 👉: doi.org/10.1038/s415...

Death penalty on the "uracil". wrong in so many ways!

Ressemble a une larve de punaise de type punaise diabolique ou punaise a pattes rousses.

Organoids stained with Spirochrome probes for actin and DNA! 😍

Painted 100+ lung organoids for Easter, stained with live-cell Cell Tracker/ @spirochrome.com dyes, imaged at our @leicamicrosystems.bsky.social Viventis Deep light-sheet 🔬 at Charité (soon @Si-M - Der Simulierte Mensch) and visualized using inverted lookup tables designed by @christlet.bsky.social

Mr Blackcap (Sylvia atricapilla) singing and dancing for Mrs Blackcap just under my window!
Turn the sound on, this little dude is a talented singer.

Mirror mirror on the wall who is the brightest of them all?

Brightness is a key parameter for choosing fluorescent reporters in bioimaging. As tricky to assess, we present a pipeline to compare chemigenetic labels and FPs in tissue culture and #zebrafish embryos.

www.biorxiv.org/content/10.6...

Beware the SPY488-FastAct rollercoaster!

We teamed up with CSR Biotech and their incredible MI-SIM microscope to drive you through the intricate actin network of live COS7 cells stained with SPY488-FastAct.

Find out more about SPY488-FastAct: spirochrome.com/product/spy4...

📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy!

There is now a very detailed #JOVE video that explains it all step by step.

Video link: dx.doi.org/10.3791/6940...

Peroxi_SPY link: spirochrome.com/products/?_c...

🧪

🤩 Another amazing example of U-ExM actin staining with HAK-actin by @dudinlab.bsky.social et. al.

How could I miss that one? Congrats Omaya!

At this high resolution, we also caught something new: Using the new #HAK-Actin #UExM probe, we saw that
#Actin accumulates at the furrow base before compartments fully seal ➡️ they're still open at the bottom.

➡️ The #multicellular epithelium-like stage is shorter than we previously proposed.

A surface rendered view of an endosome. The endosome surface is rendered in blue with dimples on it. In specific regions of the endosomal surface, clusters of proteins are seen as aggregates of blobs, coloured in yellow and red.

🚨New paper out today in @acs.org ACS Nano, showing how expansion microscopy can visually quantify the cell surface receptor turnover mediated by signalling & receptor organising machineries on the surfaces of endosomes, some of the smallest organelles in cells. 🧪 🔬 1/ pubs.acs.org/doi/10.1021/...

An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™.

These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).

Movie from preprint www.biorxiv.org/content/10.6...
🧪

5/5 The Takeaway: Flipper-TR didn't just report CMT; it identified CMT as a master switch linking physics to genetics.

From development to disease, this probe proved that how "tight" a cell is matters just as much as its DNA.

Read the full study: www.biorxiv.org/content/10.6...

🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁

Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT)

Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology

Widefield microscope image of HeLa cells stained with SPY488-FastAct

Widefield microscope image of U2OS cells stained with SPY488-FastAct (green), PKmito Orange (white), SPY650-DNA (cyan)

Widefield microscope image of U2OS cells stained with SPY488-Fastact (green) and SPY650-DNA (red)

🟢FastAct™ goes green! 🟢🔬

We launch SPY488-FastAct™ a green channel live cell actin probe

SPY488-FastAct allows imaging F-actin in live and fixed cells

Compatible with SPY555 and SPY650 for easy 3 color imaging.

spirochrome.com/product/spy4...

available at our distributors in a couple of days. 🧪

An almond tree branch tip with flower buds nearly open and one pink and delicate flower fully opened at the tip of the branch. the background is blurry with green notes on the lower half and cyan on the upper half

Almond blossom in the garden, spring upload in full swing!

Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday

Easy to use: just dilute the probe in cell culture medium and image 30 min later!

🔗 spirochrome.com/product/spy6...

🙏 Big thanks to @lazaresaladin.bsky.social for the images.
🧪

An amazing preprint by @gerlichlab.bsky.social on the fate of DNA during apoptosis. Definitely to add on your reading list!

Our SPY650-DNA probe was heavily used to visualize DNA throughout the manuscript.

Congratulations to the team!

Our article on how to use micropatterned presynapses-on-glass is out in its final form in the latest issue of the Journal of Neuroscience @sfnjournals.bsky.social: www.jneurosci.org/content/46/8...
The cover went to the special section on social neuroscience, so here's our #notthecover attempt

There's actually also one in your first SiR-tubulin movie!

seems quite common. I actually found some identical structure in one image that was sent to me.

interesting!

Another fiery 💥 probes combination by @j-today.bsky.social, this time with SPY555-Tubulin and SPY650-FastAct_X.

🤩

do you know what's that MT ring structure in the lower right quadrant that seems moving?

You are a neuron whisperer, it's really looking amazing.