Auszeichnungen für Forschende der TU Wien Ob Industrieabgase, Energietechnologien oder KI in der Medizin – Forschende der TU Wien liefern Antworten auf zentrale Fragen unserer Zeit. Drei aktuelle Auszeichnungen für Rémi Hocq, Hermann Hofbauer...

🎉 Drei Auszeichnungen für TU Wien-Forschung!
Rémi Hocq, Hermann Hofbauer und Djordje Slijepčević wurden für ihre Forschung zu Industrieabgas-Nutzung, Energietechnologien und KI in der Medizin geehrt. Wissenschaft mit Wirkung – made at TU Wien.
🔗 www.tuwien.at/tu-wien/aktu...

Revisiting carbon monoxide metabolism in the Moorella genus and isolation of a novel Moorella species Thermophilic acetogens hold great potential for syngas fermentation, as they efficiently convert CO and H2/CO2 into added-value chemical building bloc…

We cannot have enough hot acetogens. : Revisiting carbon monoxide metabolism in the Moorella genus and isolation of a novel Moorella species,
Systematic and Applied Microbiology www.sciencedirect.com/science/arti...

A megatransposon drives the adaptation of Thermoanaerobacter kivui to carbon monoxide - Nature Communications Carbon monoxide (CO) present in syngas severely inhibits growth of many acetogens. Here, the authors adapt, characterize, and engineer utilization of CO as sole carbon and energy source by the thermop...

We evolved T. kivui to grow fast on CO (doubling time ~2.8 h). The trick? A megatransposon rewired its redox and metabolic circuits.
#Transposons: not just genetic noise, but engines of innovation!
Full story: doi.org/10.1038/s414...

#Biotechnology #Extremophiles #MetabolicEngineering

Our work opens the door to real #MetabolicEngineering in T. kivui.

It grows fast on CO₂ (∼2 h doubling time) with high carbon efficiency—ideal for sustainable fuel and chemical production.

With rapid, iterative genome editing now possible, these applications are within reach!

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Hi-TARGET: a fast, efficient and versatile CRISPR type I-B genome editing tool for the thermophilic acetogen Thermoanaerobacter kivui - Biotechnology for Biofuels and Bioproducts Background Due to its ability to grow fast on CO2, CO and H2 at high temperatures and with high energy efficiency, the thermophilic acetogen Thermoanaerobacter kivui could become an attractive host fo...

✨ New publication! ✨

We made genome editing in Thermoanaerobacter kivui fast, reliable—and even cloning-free—by harnessing its own CRISPR system.

A clean edited strain can now be obtained in just 12 days!

Full story: doi.org/10.1186/s130...

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#CRISPR #SyntheticBiology #Extremophiles

Not sure you need any, PCR without the Ori with or without recircularization, and voilà ! But sacB is for sure a great approach ;)

Looks cool! Was actually wondering: if you had a non-replicative plasmid, you could perhaps save tons of time?